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Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter

Nature volume 352, pages 729–731 (1991)Cite this article

Abstract

THE complementary DNA sequence encoding the cell-surface receptor for ecotropic host-range murine retroviruses (ecoR) shows that it contains 622 amino acids and 14 hydrophobic potentially membrane-spanning sequences1. Because this receptor occurs on many or all murine cells2 and is probably essential for viability of cultured fibroblasts3, its normal function might be to transport an essential metabolite. We expressed ecoR in Xenopus laevis oocytes by injecting RNA transcribed from the cloned cDNA. These oocytes specifically bound the gp70 envelope glycoprotein from an ecotropic murine leukaemia virus. An inward current was recorded electrophysiologically when a mixture of amino-acids was applied: this resulted from a stereoselective, saturable uptake of lysine, arginine and ornithine; it was independent of sodium and not substantially altered by gp70. Cysteine and homoserine were also taken up, but sodium was necessary for their transport. These properties of ecoR correspond to those of the y+ amino-acid transporter4–6. Our results demonstrate the subversion of a ubiquitous cell membrane protein, in this case a basic amino acid transporter, for use as a retroviral receptor.

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Authors and Affiliations

  1. Department of Biochemistry, Oregon Health Sciences University, Portland, Oregon, 97201, USA

    Hao Wang & David Kabat

  2. Vollum Institute, Oregon Health Sciences University, Portland, Oregon, 97201, USA

    Michael P. Kavanaugh & R. Alan North

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  1. Hao Wang
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  2. Michael P. Kavanaugh
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  3. R. Alan North
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  4. David Kabat
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Wang, H., Kavanaugh, M., North, R. et al. Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter. Nature 352, 729–731 (1991). https://doi.org/10.1038/352729a0

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