Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Major determinants of the specificity of interaction between small nuclear ribonucleoproteins U1A and U2B" and their cognate RNAs

Nature volume 345, pages 502–506 (1990)Cite this article

Abstract

The basis of the specificity of interaction of Ul and U2 small nuclear (sn)RNAs and their cognate binding proteins, U1A and U2B", has been examined. The U1A protein recognizes Ul snRNA on its own, whereas U2B" binds specifically to U2 snRNA only in the presence of a second protein, U2A'. Exchange of two nucleotides between the two RNAs or of eight amino acids between the two proteins reverses binding specificity.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Bandziulis, R. J., Swanson, M. S. & Dreyfuss, G. Genes Dev. 3, 431–437 (1989).

    Article  CAS  Google Scholar 

  2. Mattaj, I. W. Cell 57, 1–3 (1989).

    Article  CAS  Google Scholar 

  3. Theissen, H. et al. EMBO J. 5, 3209–3217 (1986).

    Article  CAS  Google Scholar 

  4. Adam, S. A. et al. Molec. cell. Biol. 6, 2932–2943 (1986).

    Article  CAS  Google Scholar 

  5. Dreyfuss, G., Swanson, M. S. & Pinol-Roma, S. Trends biochem. Sci. 13, 86–91 (1988).

    Article  CAS  Google Scholar 

  6. Query, C. C., Bentley, R. C. & Keene, J. D. Cell 57, 89–101 (1989).

    Article  CAS  Google Scholar 

  7. Query, C. C. & Keene, J. D. Cell 51, 211–220 (1987).

    Article  CAS  Google Scholar 

  8. Spritz, R. A. et al. Nucleic Acids Res. 15, 10373–10391 (1987).

    Article  CAS  Google Scholar 

  9. Sillekens, P. T. et al. EMBO J. 6, 3841–3848.

  10. Habets, W. J. et al. Proc. natn. Acad. Sci. U.S.A. 84, 2421–2425 (1987).

    Article  ADS  CAS  Google Scholar 

  11. Scher ly, D. et al. EMBO J. 8, 4163–4170 (1989).

    Article  Google Scholar 

  12. Lutz-Freyermuth, C. & Keene, J. D. Molec. Cell Biol. 9, 2975–2982.

  13. Mattaj, I. W. & De Robertis, E. M. Cell 40, 111–118 (1985).

    Article  CAS  Google Scholar 

  14. Mattaj, I. W., Habets, W. J. & van Venrooij, W. J. EMBO J. 5, 997–1002 (1986).

    Article  CAS  Google Scholar 

  15. Hamm, J., Dathan, N. A. & Mattaj, I. W. Cell 59, 159–169 (1989).

    Article  CAS  Google Scholar 

  16. Sillekens, P. T. G. et al. Nucleic Acids Res. 17, 1983–1906 (1989).

    Article  Google Scholar 

  17. Keller, E. B. & Noon, W. A. Nucleic Acids Res. 13, 4971–4981 (1985).

    Article  CAS  Google Scholar 

  18. Igel, A. H. & Ares, M. Jr Nature 334, 450–453 (1988).

    Article  ADS  CAS  Google Scholar 

  19. Guthrie, C. & Patterson, B. A. Rev. Genet. 22, 387–419 (1988).

    Article  CAS  Google Scholar 

  20. Mattaj, I. W. & Zeller, R. EMBO J. 2, 1883–1891 (1983).

    Article  CAS  Google Scholar 

  21. Zeller, R. et al. EMBO J. 3, 1075–1081 (1984).

    Article  CAS  Google Scholar 

  22. Shuster, E. O. & Guthrie, C. Cell 55, 41–48 (1988).

    Article  CAS  Google Scholar 

  23. Chan, E. K. L., Sullivan, K. F. & Tan, E. M. Nucleic Acids Res. 17, 2233–2244 (1989).

    Article  CAS  Google Scholar 

  24. Wharton, R. P. & Ptashne, M. Nature 316, 601–605 (1985).

    Article  ADS  CAS  Google Scholar 

  25. Hanes, S. D. & Brent, R. Cell 57, 1275–1283 (1989).

    Article  CAS  Google Scholar 

  26. Danielsen, M., Hinck, L. & Ringold, G. M. Cell 57, 1131–1138 (1989).

    Article  CAS  Google Scholar 

  27. Marder, S. et al. Nature 338, 271–274 (1989).

    Article  ADS  Google Scholar 

  28. Umesono, K. & Evans, R. M. Cell 57, 1139–1146 (1989).

    Article  CAS  Google Scholar 

  29. Brennan, R. G. & Matthews, B. W. J. biol. Chem. 264, 1903–1906 (1989).

    CAS  PubMed  Google Scholar 

  30. Hamm, J., Kazmaier, M. & Mattaj, I. W. EMBO J. 6, 3479–3485 (1987).

    Article  CAS  Google Scholar 

  31. Dignam, J. D., Lebowitz, R. M. & Roeder, R. G. Nucleic Acids Res. 11, 1475–1489 (1983).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. W. Boelens: University of Nijmegen, Department of Biochemistry, PO Box 9101, 6500 HB Nijmegen, The Netherlands

Authors and Affiliations

  1. European Molecular Biology Laboratory, Meyerhofstrasse 1, 6900, Heidelberg, FRG

    D. Scherly, W. Boelens, N. A. Dathan, W. J. van Venrooij & I. W. Mattaj

Authors
  1. D. Scherly
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Boelens
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. A. Dathan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. W. J. van Venrooij
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. W. Mattaj
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scherly, D., Boelens, W., Dathan, N. et al. Major determinants of the specificity of interaction between small nuclear ribonucleoproteins U1A and U2B" and their cognate RNAs. Nature 345, 502–506 (1990). https://doi.org/10.1038/345502a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/345502a0

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing