Abstract
SEVERAL genes encoding putative myogenic regulatory factors have been isolated on the basis of their ability to convert non-muscle cells into myoblasts1–6. Four of these genes code for nuclear proteins that belong to a larger family characterized by a conserved helix–loop–helix motif required for DNA-binding and dimerization7. At least one protein, MyoD1, can function as a transcription factor and activate muscle-specific genes during differentiation8. But the promoter of the δ-subunit gene of the mouse acetylcholine receptor (AChR) was recently reported to be functional in the absence of MyoDl binding sites and it has been suggested that the genes coding for the AChR could be regulated independently of MyoDl protein9. Here, we identify two functional MyoDl-binding sites in the muscle-specific enhancer of the chicken AChR α-subunit gene that are essential for full activity in transfected myotubes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Davis, R. L., Weintraub, H. & Lassar, A. B. Cell 51, 987–1000 (1987).
Pinney, D. F., Pearson-White, S. M., Konieczny, S. F., Latham, K. E. & Emerson, C. P. Jr Cell 53, 781–793 (1988).
Wright, W. E., Sassoon, D. A. & Lin, V. K. Cell 56, 607–617 (1989).
Braun, T., Buschhausen-Denker, G., Bober, E., Tannich, E. & Arnold, H. H. EMBO J. 8, 701–709 (1989).
Rhodes, S. J. & Konieczny, S. F. Genes Dev. 3, 2050–2061 (1989).
Colmenares, C. & Stavnezer, E. Cell 59, 293–303 (1989).
Murre, C., McCaw, P. S. & Baltimore, D. Cell 56, 777–783 (1989).
Lassar, A. B. et al. Cell 58, 823–831 (1989).
Baldwin, T. J. & Burden, S. J. Nature 341, 716–720 (1989); correction Nature 345, 364 (1990).
Klarsfeld, A., Daubas, P., Bourachot, B. & Changeux, J.-P. Molec. cell. Biol. 7, 951–955 (1987).
Daubas, P. et al. Nucleic Acids Res. 16, 1251–1271 (1988).
Wang, Y., Xu, H. P., Wang, X. M., Ballivet, M. & Schmidt, J. Neuron 1, 527–534 (1988).
Piette, J., Klarsfeld, A. & Changeux, J.-P. EMBO J. 8, 687–694 (1989).
Tapscott, S. J. et al. Science 242, 405–441 (1988).
Montarras, D., Pinset, C. Chelly, J., Kahn, A. & Gros, F. EMBO J. 8, 2203–2207 (1989).
Duclert, A., Piette, J. & Changeux, J.-P. Proc. natn. Acad. Sci. U.S.A. 87, 1392–1395 (1990).
Changeux, J. P. et al. NATO ASI Series Vol. H32 (Springer, Berlin, 1989).
Graham, F. L. & Vander Eb, A. J. Virology 52, 456–467 (1973).
Seed, B. & Sheen, J.-Y. Gene 67, 271–277 (1988).
Tronche, F. et al. Molec. Biol. Med. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Piette, J., Bessereau, JL., Huchet, M. et al. Two adjacent MyoD1-binding sites regulate expression of the acetylcholine receptor α-subunit gene. Nature 345, 353–355 (1990). https://doi.org/10.1038/345353a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/345353a0
This article is cited by
-
Cloning and expression of MyoG gene from Hu sheep and identification of its myogenic specificity
Molecular Biology Reports (2014)
-
Postnatal muscle modification by myogenic factors modulates neuropathology and survival in an ALS mouse model
Nature Communications (2013)
-
miR-206 integrates multiple components of differentiation pathways to control the transition from growth to differentiation in rhabdomyosarcoma cells
Skeletal Muscle (2012)
-
Mechano growth factor (MGF) promotes proliferation and inhibits differentiation of porcine satellite cells (PSCs) by down-regulation of key myogenic transcriptional factors
Molecular and Cellular Biochemistry (2012)
-
Synergistic up-regulation of muscle LIM protein expression in C2C12 and NIH3T3 cells by myogenin and MEF2C
Molecular Genetics and Genomics (2009)
