Abstract
RECENT experiments have implicated intracellular events in the formation of the MHC class II-peptide complexes recognized by CD4-positive T cells1–6. These data raise the possibility that the intracellular association of class II with the non-polymorphic glycoprotein, invariant chain (Ii)7,8, may regulate the interaction between processed antigen and MHC class II molecules. To address this possibility, we have generated a series of transfected fibroblast cell lines that express class II with and without Ii. Although the presence of Ii does not seem to affect the ability of the cells to process and present intact antigen, Ii-negative cells express an altered form of class II at the cell surface. This modified conformation of class II in Ii-negative cells is detectable by an increase in the ability to present antigenic peptides to T cells and a decrease in the binding of several class II-specific monoclonal antibodies.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Unanue, E. A. Rev. Immun. 2, 395–428 (1984).
Buus, S., Sette, A., Colon, S., Jenis, D. & Grey, H. Cell 47, 1071–1077 (1986).
Morrison, L., Lukacher, A., Brachiale, V., Fan, D. & Brachiale, T. J. exp. Med. 163, 903–921 (1986).
Cresswell, P. Proc. natn. Acad. Sci. U.S.A. 82, 8188–8192 (1985).
Harding, C. & Unanue, E. J. Immun. 142, 12–19 (1989).
Jensen, P. J. immun. 141, 2545–2550 (1988).
Kvist, S., Wiman, K., Claesson, L., Peterson, P. & Dobberstein, B. Cell 29, 61–69 (1982).
Holt, G., Swiedler, S., Freed, J. & Hart, G. J. Immun. 135, 399–407 (1985).
Miller, J. & Germain, R. J. exp. Med 164, 1478–1489 (1986).
Sekaly, R., Tonnelle, C., Strubin, M., Mach, B. & Long, E. J. exp. Med. 164, 1490–1504 (1986).
Simonis, S., Miller, J. & Cullen, S. J. Immun. 143, 3619–3625 (1989).
Shimonkevitz, R., Kappler, J., Marrack, P. & Grey, H. J. exp. Med. 158, 303–316 (1983).
Townsend, A. et al. Nature 340, 443–448 (1989).
Mellins, E. et al. Nature 343, 71–74 (1990).
Germain, R. & Malissen, B. A. Rev. Immun. 4, 281–315 (1986).
Stockinger, B. et al. Cell 56, 683–689 (1989).
Miller, J., Hatch, J., Simonis, S. & Cullen, S. Proc. natn. Acad. Sci. U.S.A. 85, 1359–1363 (1988).
Glimcher, L., Sohroer, J., Chan, C. & Shevach, E. J. Immun. 131, 2868–2874 (1983).
Kappler, J., Scidmore, B., White, J. & Marrack, P. J. exp. Med. 153, 1198–1214 (1981).
Ozato, K., Mayer, N. & Sachs, D. Transplantation 34, 113–120 (1982).
Bhattacharya, A., Dorf, M. & Springer, T. J. Immun. 127, 2488–2495 (1981).
Ozato, K. & Sachs, D. J. Immun. 126, 317–321 (1981).
Koch, N., Hammerling, G., Tada, N., Kimura, S. & Hammerling, U. Eur. J. Immun. 12, 909–914 (1982).
Pierres, M., Devaux, C., Dosseto, M. & Marchetto, S. Immunogenetics 14, 481–495 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Peterson, M., Miller, J. Invariant chain influences the immunological recognition of MHC class II molecules. Nature 345, 172–174 (1990). https://doi.org/10.1038/345172a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/345172a0
This article is cited by
-
pRB is required for interferon-γ-induction of the MHC class II Aβ gene
Oncogene (1999)
-
Protein sorting within the mhc class II antigen-processing pathway
Immunologic Research (1998)
-
Antimalarial Drugs
Clinical Immunotherapeutics (1995)
-
Molecular chaperones in the processing and presentation of antigen to helper T cells
Experientia (1994)
-
Endosomal localization of MHC class II-invariant chain complexes
Immunologic Research (1994)