Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate

Nature volume 336, pages 738–744 (1988)Cite this article

Abstract

cdc2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells, cdc2 is shown to be the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell-cycle regulation. One site ofcdc2 tyrosine phosphorylation in vivo W selectively phosphorylated by pp60c–src in vitro.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Greenberg, M. & Ziff, E. Nature 311, 433–438 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Bravo, R. & McDonald-Bravo, H. EMBO J. 4, 655–661 (1985).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Bishop, J. M. Cell 42, 23–38 (1985).

    Article  CAS  PubMed  Google Scholar 

  4. Harwell, L. H. Bact. Rev. 38, 164–198 (1974).

    Google Scholar 

  5. Reed, S. I. Genetics 95, 561–577 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Lorincz, A. T. & Reed, S. I. Nature 307, 183–185 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Beach, D. H., Durkacz, B. & Nurse, P. Nature 300, 706–709 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Simanis, V. & Nurse, P. Cell 45, 261–268 (1986).

    Article  CAS  PubMed  Google Scholar 

  9. Brizuela, L., Draetta, G. & Beach, D. EMBO J. 6, 3507–3514 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Draetta, G., Brizuela, L., Potashkin, J. & Beach, D. Cell 50, 319–325 (1987).

    Article  CAS  PubMed  Google Scholar 

  11. Lee, M. & Nurse, P. Nature 327, 31–35 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Hindley, J., Phear, G. A., Stein, M. & Beach, D. Molec. cell. Biol. 7, 504–511 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Draetta, G. & Beach, D. Cell 54, 17–26 (1988).

    Article  CAS  PubMed  Google Scholar 

  14. Nurse, P. & Bissett, Y. Nature 292, 558–560 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  15. Dunphy, W., Brizuela, L., Beach, D. & Newport, J. Cell 54, 423–431 (1988).

    Article  CAS  PubMed  Google Scholar 

  16. Gautier, J., Norbury, C., Lohka, M., Nurse, P. & Maller, J. Cell 54, 433–439 (1988).

    Article  CAS  PubMed  Google Scholar 

  17. Smith, L. D. & Ecker, R. E. Devl Biol. 25, 232–247 (1971).

    Article  CAS  Google Scholar 

  18. Masui, Y. & Markert, C. L. J. exp. Zool. 177, 129–146 (1971).

    Article  CAS  PubMed  Google Scholar 

  19. Labbe, J., Lee, M., Nurse, P., Picard, A. & Doree, M. Nature 335, 251–254 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Arion, D., Meijer, L., Brizuela, L. & Beach, D. Cell 55, 371–378 (1988).

    Article  CAS  PubMed  Google Scholar 

  21. Draetta, G. (in preparation).

  22. Evans, T. Rosenthal, E. T., Youngman, J., Distel, D. & Hunt, T. Cell 33, 389–396 (1983).

    Article  CAS  PubMed  Google Scholar 

  23. Morrison, D. K., Browning, P. J., White, M. F. & Roberts, T. M. Molec. cell. Biol. 8, 176–185 (1988).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Chackalaparampil, I. & Shalloway, D. Cell 52, 801–810 (1988).

    Article  CAS  PubMed  Google Scholar 

  25. Jore, R., Kornbluth, S. & Hanafusa, H. Cell 50, 937–943 (1987).

    Article  Google Scholar 

  26. Lischwe, M. A. & Ochs, D. Analyt. Biochem. 127, 453–457 (1982).

    Article  CAS  PubMed  Google Scholar 

  27. Hindley, J. & Phear, G. A. Gene 31, 129–134 (1984).

    Article  CAS  PubMed  Google Scholar 

  28. Miake-Lye, R. & Kirschner, M. Cell 1, 165–175 (1985).

    Article  Google Scholar 

  29. Sunkara, P. S., Wright, D. A. & Rao, P. N. Proc. natn. Acad. Sci. U.S.A. 76, 2799–2802 (1979).

    Article  ADS  CAS  Google Scholar 

  30. Coussens, P. M., Cooper, J. A., Hunter, T. & Shalloway, D. Molec. cell. Biol. 5, 2753–2763 (1985).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Spivak, J., Erikson, R. & Maller L. J. Molec. cell. Biol. 4, 1631–1634 (1984).

    Article  Google Scholar 

  32. Melchers, F. & Andersson, J. Cell 37, 715–720 (1984).

    Article  CAS  Google Scholar 

  33. Melchers, F. & Lernhardt, W. Proc. natn. Acad. Sci. U.S.A. 82, 7681–7685 (1985).

    Article  ADS  CAS  Google Scholar 

  34. Gelfant, S. Symp. Int. Soc. cell. Biol. 2, 229–259 (1963).

    Google Scholar 

  35. Costello, G., Rodgers, L. & Beach, D. Curr. Genet. 11, 119–125 (1986).

    Article  Google Scholar 

  36. Prescott, D. M. Reproduction of Eukaryotic Cells 177 (Academic, New York, 1976).

    Google Scholar 

  37. Drewinko, B., Yang, L. Y., Barlogie, B. & Trujillo, J. M. Cell Tissue Kinet. 17, 453–463.

  38. Booher, R. & Beach, D. EMBO J. 6, 3441–3447 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Beemon, K. & Hunter, T. J. Virol. 28, 551–566 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  40. Piwnica-Worms, H., Kaplan, D. R., Whitman, M. & Roberts, T. M. Molec. cell. Biol. 6, 2033–2040 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Summers, M. D. & Smith, G. E. in A Manual of Methods for Baculovirus Vectors and Cloned Insect Cell Culture Procedures (Texas Agricultural Station, College Station, Texas, 1987).

    Google Scholar 

  42. Hunter, T. & Sefton, B. Proc. natn. Acad. Sci. U.S.A., 77, 1311–1315 (1980).

    Article  ADS  CAS  Google Scholar 

  43. Pallas, D. P. & Solomon, F. Cell 30, 407–414 (1982).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cold Spring Harbor Laboratory, PO Box 100, Cold Spring Harbor, New York, 11724, USA

    Giulio Draetta & David Beach

  2. Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Helen Piwnica-Worms, Deborah Morrison, Brian Druker & Thomas Roberts

Authors
  1. Giulio Draetta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Helen Piwnica-Worms
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Deborah Morrison
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Brian Druker
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Thomas Roberts
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. David Beach
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Draetta, G., Piwnica-Worms, H., Morrison, D. et al. Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate. Nature 336, 738–744 (1988). https://doi.org/10.1038/336738a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/336738a0

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing