Abstract
In eukaryotes the entire genome is replicated precisely once in each cell cycle. No DNA is re-replicated until passage through mitosis into the next S-phase. We have used a cell-free DNA replication system from Xenopus eggs to determine which mitotic changes permit DNA to re-replicate. The system efficiently replicates sperm chromatin, but no DNA is re-replicated in a single incubation1. This letter shows that nuclei replicated in vitro are unable to re-replicate in fresh replication extract until they have passed through mitosis. However, the only mitotic change which is required to permit re-replication is nuclear envelope permeabiliz-ation. This suggests a simple model for the control of DNA replication in the cell cycle, whereby an essential replication factor is unable to cross the nuclear envelope but can only gain access to DNA when the nuclear envelope breaks down at mitosis.
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References
Blow, J. J. & Laskey, R. A. Cell 47, 577–587 (1986).
Lohka, M. J. & Masui, Y. Science 220, 719–721 (1983).
Lohka, M. J. & Masui, Y. J. Cell Biol. 98, 1222–1230 (1984).
Sheehan, M. A., Mills, A. D., Sleeman, A. M., Laskey, R. A. & Blow, J. J. J. Cell Biol. 106, 1–12 (1988).
Blow, J. J. & Watson, J. V. EMBO J. 6, 1997–2002 (1987).
Newport, J. Cell 48, 205–217 (1987).
Hutchison, C. J., Cox, R., Drepaul, R. S., Gomperts, M. & Ford, C. C. EMBO J. 6, 2003–2010 (1987).
Newport, J. & Kirschner, M. W. Cell 37, 731–741 (1984).
Miake-Lye, R. & Kirschner, M. Cell 41, 165–175 (1985).
Lohka, M. J. & Masui, Y. Devl Biol. 103, 434–442 (1984).
Newport, J. & Spann, T. Cell 48, 219–230 (1987).
Laskey, R. A., Harland, R. M., Ernshaw, W. C. & Dingwall, C. in International Cell Biology 1980–1981 (ed. Schwieger, H. G.) 161–167 (Springer, Berlin, 1981).
Blow, J. J., Dilworth, S. M., Dingwall, C., Mills, A. D. & Laskey, R. A. Phil. Trans. R. Soc. B317, 483–494 (1987).
Blow, J. J., Sheehan, M. A., Watson, J. V. & Laskey, R. A. Cancer Cells 6, (Cold Spring Harbor Laboratory, New York, in the press).
Rao, P. N. & Johnson, R. T. Nature 225, 159–164 (1970).
Harland, R. M. & Laskey, R. A. Cell 21, 761–771 (1980).
Mechali, M. & Kearsey, S. Cell 38, 55–64 (1984).
Forbes, D. J., Kirschner, M. W. & Newport, J. W. Cell 34, 13–23 (1983).
Newmeyer, D. D., Lucocq, J. J., Burglin, T. R. & De Robertis, E. M. EMBO J. 5, 501–510 (1986).
Mechali, M., Mechali, F. & Laskey, R. A. Cell 35, 63–69 (1983).
Roberts, J. M. & Weintraub, H. Cell 46, 741–752 (1986).
Berg, L., Lusky, M., Stenlund, A. & Botchan, M. R. Cell 46, 753–762 (1986).
De Roeper, A., Smith, J. A., Watt, R. A. & Barry, J. M. Nature 265, 469–470 (1977).
Byers, B. in The Molecular Biology of the Yeast Saccharomyces (eds Strathern, J. N., Jones, E. W. & Broach, J. R.) 59–96 (Cold Spring Harbor Laboratory, New York, 1981).
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Blow, J., Laskey, R. A role for the nuclear envelope in controlling DNA replication within the cell cycle. Nature 332, 546–548 (1988). https://doi.org/10.1038/332546a0
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DOI: https://doi.org/10.1038/332546a0
