Abstract
Cell-free extracts prepared from human 293 cells, supplemented with purified SV40 large-T antigen, support replication of plasmids containing the SV40 origin of DNA replication. A cellular protein (Mr ∼36,000) that is required for efficient SV40 DNA synthesis in vitro has been purified from these extracts. This protein is recognized by human autoantibodies and is identified as the cell-cycle regulated protein known as proliferating cell nuclear antigen (PCNA) or cyclin.
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References
Kornberg, A. DNA Replication (Freeman, San Francisco, 1980).
Kornberg, A. DNA Replication: 1982 Supplement (Freeman, San Francisco, 1982).
Marians, K. J. CRC crit. Rev. Biochem. 17, 153–215 (1984).
Challberg, M. D. & Kelly, T. J. Jr Proc. natn. Acad. Sci. U.S.A. 76, 655–659 (1979).
Li, J. J. & Kelly, T. J. Proc. natn. Acad. Sci. U.S.A. 81, 6973–6977 (1984).
Li, J. J. & Kelly, T. J. Molec. cell. Biol. 5, 1238–1246 (1985).
Stillman, B. W. & Gluzman, Y. Molec. cell. Biol. 5, 2051–2060 (1985).
Wobbe, C. R., Dean, F., Weissbach, L. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 82, 5710–5714 (1985).
Murakami, Y., Eki, T., Yamada, M., Prives, C. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 83, 6347–6351 (1986).
DePamphilis, M. L. & Wassarman, P. M. in Organization and Replication of Viral DNA (ed. Kaplan, A. S.) 37–114 (CRC Press, Florida, 1982).
Sundin, O. & Varshavsky, A. Cell 25, 659–669 (1981).
Weaver, D. T., Fields-Berry, S. C. & DePamphilis, M. L. Cell 41, 565–575 (1985).
Tegtmeyer, P. J. Virol. 10, 591–598 (1972).
Tegtmeyer, P., Schwartz, M., Collins, J. K. & Rundell, K. J. Virol. 16, 168–178 (1975).
Myers, R. M., Rio, D. C., Robbins, A. K. & Tjian, R. Cell 25, 373–384 (1981).
Hansen, U., Tenen, D. G., Livingston, D. M. & Sharp, P. A. Cell 27, 603–612 (1981).
Tooze, J. Molecular Biology of Tumour Viruses 2nd edn, part 2 (Cold Spring Harbor Laboratory, New York, 1980).
Rigby, P. W. J. & Lane, D. P. Adv. viral Oncol. 3, 31–57 (1983).
Tjian, R. Cell 26, 1–2 (1981).
Stahl, H., Droge, P. & Knippers, R. EMBO J. 5, 1939–1944 (1986).
Clark, R., Peden, K., Pipas, J., Nathans, D. & Tjian, R. Molec. cell Biol. 3, 220–228 (1983).
Kalderon, D. & Smith, A. E. Virology 139, 109–137 (1984).
Stillman, B., Gerard, R. D., Guggenheimer, R. A. & Gluzman, Y. EMBO J. 4, 2933–2939 (1985).
Stillman, B. Cell 45, 555–565 (1986).
Miyachi, K., Fritzler, M. J. & Tan, E. M. J. Immun. 121, 2228–2234 (1978).
Takasaki, Y., Fishwild, D. & Tan, E. M. J. exp. Med. 159, 981–992 (1984).
Tan, E. M. Adv. Immun. 33, 167–240 (1982).
Mathews, M. B., Bernstein, R. M., Franza, B. R. Jr & Garrels, J.I. Nature 309, 374–376 (1984).
Prelich, G. et al. Nature 326, 517–520 (1987).
Stillman, B. W., Diffley, J. F. X., Prelich, G. & Guggenheimer, R. A. in Cancer Cells 4 DNA Tumour Viruses: Control of Gene Expression and Replication (eds Botchan, M., Grodzicker, T. & Sharp, P.) 453–463 (Cold Spring Harbor Laboratory, New York, 1986).
Takasaki, Y., Robinson, W. A. & Tan, E. M. J. natn. Cancer Inst. 73, 655–661 (1984).
Smetana, K., Gyorkey, F., Chan, P. K., Tan, E. & Busch, H. Blut 46, 133–141 (1983).
Bravo, R. & Celis, J. E. J. Cell Biol. 84, 795–802 (1980).
Bravo, R. & McDonald-Bravo, H. EMBO J. 4, 655–661 (1985).
Bravo, R. Expl Cell Res. 163, 287–293 (1986).
Ogata, K., Ogata, Y., Nakamura, R. M. & Tan, E. M. J. Immun. 135, 2623–2627 (1985).
Celis, J. E., & Celis, A. Proc. natn. Acad. Sci. U.S.A. 82, 3262–3266 (1985).
Kurki, P., Vanderlaan, M., Dolbeare, F., Gray, J. & Tan, E. M. Expl Cell Res. 166, 209–219 (1986).
Madsen, P. & Celis, J. E. FEBS Lett. 193, 5–11 (1985).
Sadaie, M. R. & Mathews, M. B. Expl Cell Res. 163, 423–433 (1986).
Wobbe, C. R., Dean, F. B., Murakami, Y., Weissbach, L. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 83, 4612–4616 (1986).
Celis, J. E., Madsen, P., Nielsen, S. & Celis, A. Leukemia Res. 10, 237–249 (1986).
Zerler, B., Roberts, R. J., Mathews, M. B. & Moran, E. Molec. cell. Biol. 7, 821–829 (1987).
Tan, C., Castillo, C., So, A. G. & Downey, K. M. J. biol. Chem. 261, 12310–12316 (1986).
Bradford, M. M. Analyt. Biochem. 72, 248–254 (1976).
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Prelich, G., Kostura, M., Marshak, D. et al. The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro. Nature 326, 471–475 (1987). https://doi.org/10.1038/326471a0
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DOI: https://doi.org/10.1038/326471a0