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The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro

Nature volume 326, pages 471–475 (1987)Cite this article

Abstract

Cell-free extracts prepared from human 293 cells, supplemented with purified SV40 large-T antigen, support replication of plasmids containing the SV40 origin of DNA replication. A cellular protein (Mr ∼36,000) that is required for efficient SV40 DNA synthesis in vitro has been purified from these extracts. This protein is recognized by human autoantibodies and is identified as the cell-cycle regulated protein known as proliferating cell nuclear antigen (PCNA) or cyclin.

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Author notes

  1. Bruce Stillman: To whom correspondence should be addressed.

Authors and Affiliations

  1. Cold Spring Harbor Laboratory, PO Box 100, Cold Spring Harbor, New York, 11724, USA

    Gregory Prelich, Matthew Kostura, Daniel R. Marshak, Michael B. Mathews & Bruce Stillman

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  1. Gregory Prelich
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  2. Matthew Kostura
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  3. Daniel R. Marshak
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  4. Michael B. Mathews
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  5. Bruce Stillman
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Prelich, G., Kostura, M., Marshak, D. et al. The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro. Nature 326, 471–475 (1987). https://doi.org/10.1038/326471a0

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