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Antibodies against platelet-derived growth factor inhibit acute transformation by simian sarcoma virus

Nature volume 317, pages 438–440 (1985)Cite this article

Abstract

A clue to the molecular mechanism of neoplastic transformation was provided by the finding of a near identity in amino-acid sequence between the platelet-derived growth factor (PDGF) B-chain and a region in the transforming protein, p28sis, of simian sarcoma virus (SSV)1–4, an agent that causes sarcomas and gliomas in experimental animals5,6. This finding infers a direct link between the molecular biology of normal mitogenesis and oncogenesis since it suggests that the transforming activity of SSV is caused by a growth factor. Although PDGF agonist activity has been isolated from conditioned medium of SSV-transformed cells7–10, it is not clear whether infection of responsive cells by SSV leads solely to autocrine stimulation of growth by a secreted PDGF-like factor or whether other, possibly intracellular, activities of p28sis or its processed products contribute to the transformation9,11,12. To distinguish between these possibilities, we have studied the effect of anti-PDGF antibodies on acute SSV-transformation, and report here that these antibodies inhibit both proliferation and SSV-induced morphological changes in human diploid fibroblasts.

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Authors and Affiliations

  1. Department of Medical and Physiological Chemistry, University of Uppsala, S-751 23, Uppsala, Sweden

    Ann Johnsson & Carl-Henrik Heldin

  2. Department of Pathology, University of Uppsala, S-751 23, Uppsala, Sweden

    Christer Betsholtz & Bengt Westermark

Authors
  1. Ann Johnsson
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  2. Christer Betsholtz
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  3. Carl-Henrik Heldin
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  4. Bengt Westermark
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Johnsson, A., Betsholtz, C., Heldin, CH. et al. Antibodies against platelet-derived growth factor inhibit acute transformation by simian sarcoma virus. Nature 317, 438–440 (1985). https://doi.org/10.1038/317438a0

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