Abstract
Three mutations that alter the DNA sequence specificity of the catabolite gene activator protein (CAP) from AA-TGTGA--T---TCA-ATW to AA-TGTAA--T---TCA-ATW have been isolated. All three mutations affect the same amino acid of CAP, glutamic acid 181. We propose that it is this amino acid of CAP that makes contacts with base pairs 7 and 16 of the symmetrical recognition site.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Pastan, I. & Adhya, S. Bact. Rev. 40, 527–551 (1976).
DeCrombrugghe, B., Busby, S. & Buc, H. Science 224, 831–838 (1984).
Majors, J. thesis, Harvard Univ. (1975).
Simpson, R. Nucleic Acids Res. 8, 759–766 (1980).
Beckwith, J., Grodzicker, T. & Arditti, R. J. molec. Biol. 69, 115–160 (1972).
Dickson, R. et al. J. molec. Biol. 111, 65–76 (1977).
Majors, J. Nature 256, 672–674 (1975).
Kolb, A. et al. EMBO J. 2, 217–222 (1983).
Ebright, R., Cossart, P., Gicquel-Sanzey, B. & Beckwith, J. Proc. natn. Acad. Sci. U.S.A. (in the press).
Alper, M. & Ames, B. J. Bact. 133, 149–157 (1978).
Kolb, A. et al. Nucleic Acids Res. 11, 7833–7852 (1983).
Kahan, F., Kahan, J., Cassidy, P. & Kropp, H. Ann. N. Y. Acad. Sci. 235, 364–386 (1974).
Lederberg, J. Genetics 32, 505–524 (1947).
Legerski, R., Hodnett, J. & Gray, H. Nucleic Acids Res. 5, 1445–1463 (1978).
Maxam, A. & Gilbert, W. Meth. Enzym. 68, 499–560 (1980).
McKay, D. & Steitz, T. Nature 290, 744–749 (1981).
McKay, D., Weber, I. & Steitz, T. J. biol. Chem. 257, 9518–9524 (1982).
Steitz, T., Weber, I. & Matthew, J. Cold Spring Harb. Symp. quant. Biol. 47, 419–426 (1983).
Steitz, T. et al. Proc. natn. Acad. Sci. U.S.A. 79, 3097–3100 (1982).
Ohlendorf, D. et al. J. molec. Biol. 169, 757–769 (1983).
Anderson, W., Ohlendorf, D., Takeda, Y. & Matthews, B. Nature 290, 754–758 (1981).
Pabo, C. & Lewis, M. Nature 298, 443–147 (1982).
Anderson, W., Takeda, Y., Ohlendorf, D. & Matthews, B. J. molec. Biol. 159, 745–751 (1982).
Sauer, R. et al. Nature 298, 447–451 (1982).
Weber, I., McKay, D. & Steitz, T. Nucleic Acids Res. 10, 5085–5102 (1982).
Eilen, E., Pampeno, C. & Krakow, J. Biochemistry 17, 2469–2474 (1978).
Pampeno, C. & Krakow, J. Fedn Proc. 37, 1830 (1978).
Perutz, M. & Lehmann, H. Nature 219, 902–909 (1968).
Hecht, M., Nelson, H. & Sauer, R. Proc. natn. Acad. Sci U.S.A. 80, 2676–2680 (1983).
Chou, P. & Fasman, G. Biochemistry 13, 222–245 (1974).
Youderian, P. et al. Cell 35, 777–783 (1983).
Miller, J. Experiments in Molecular Genetics (Cold Spring Harbor Laboratory, New York, 1972).
Signer, E., Beckwith, J. & Brenner, S. J. molec. Biol. 14, 153–166 (1965).
Kovacs, H. Z. Immun. 55, 311–315 (1928).
Brickman, E., Soll, L. & Beckwith, J. J. Bact. 116, 582–587 (1973).
Ebright, R. in Molecular Structure and Biological Activity (eds Griffen, J. & Duax, W.) 91–100 (Elsevier, New York, 1982).
Chapon, C. & Kolb, A. J. Bact. 156, 1135–1143 (1983).
Stoner, C. & Schleif, R. J. molec. Biol. 171, 369–381 (1983).
Deeley, M. & Yanofsky, C. J. Bact. 151, 942–951 (1982).
Friden, D., Newman, T. & Freundlich, M. Proc. natn. Acad. Sci. U.S.A. 79, 6156–6160 (1980).
Aiba, H. Cell 32, 141–149 (1983).
Hopkins, J. J. molec. Biol. 87, 715–724 (1974).
Aiba, H., Fujimoto, S. & Ozaki, N. Nucleic Acids Res. 10, 1345–1362 (1982).
Cossart, P. & Gicquel-Sanzey, B. Nucleic Acids Res. 10, 1363–1378 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ebright, R., Cossart, P., Gicquel-Sanzey, B. et al. Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli. Nature 311, 232–235 (1984). https://doi.org/10.1038/311232a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/311232a0
