Abstract
Cell strains derived from patients having a hereditary disorder associated with defects in repair of DNA damage such as xeroderma pigmentosum1,2 and mutants isolated from established rodent cell lines3,4 provide the tools for genetic and biochemical analysis of DNA repair pathways in mammalian cells. Complementation studies using these cells have illustrated the genetic and biochemical complexity of these pathways3,5,6. The precise nature of the genes and gene products involved in these mutants has not yet been resolved. Isolation of repair genes by recombinant DNA technology would open up new approaches to the elucidation of repair mechanisms in mammalian cells. Here we report the molecular cloning of a human repair gene (ERCC1) that complements the repair defect in a Chinese hamster ovary (CHO) mutant cell line.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Kraemer, K. H. in Update: Dermatology in General Medicine (eds Fitzpatrick, T. B., Eisen, A. Z., Wolff, K., Freedberg, I. M. & Austen, K. F.) 113–141 (McGraw-Hill, New York, 1983).
Lehmann, A. R. & Karran, P. Int. Rev. Cytol. 72, 101–146 (1981).
Thompson, L. H., Busch, D. B., Brookman, K., Mooney, C. L. & Glaser, D. A. Proc. natn. Acad. Sci. U.S.A. 78, 3734–3737 (1981).
Shiomi, T., Hieda-Shiomi, N. & Sato, K. Somat. Cell Genet. 8, 329–345 (1980).
de Weerd-Kastelein, E. A., Keijzer, W. & Bootsma, D. Nature new Biol. 238, 80–83 (1972).
Moshell, A. N. et al. in Cellular Responses to DNA Damage (eds Friedberg, E. C. & Bridges, B.) 209–213 (Liss, New York, 1983).
Wood, R. D. & Burki, H. J. Mutat. Res. 95, 505–514 (1982).
Rubin, J. S., Joyner, A. L., Bernstein, A. & Whitmore, G. F. Nature 306, 206–208 (1983).
Mulligan, R. C. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 78, 2072–2076 (1981).
Wigler, M. et al. Cell 16, 777–785 (1979).
Lowy, I. et al. Cell 22, 817–823 (1980).
Pellicer, A. et al. Science 209, 1414–1422 (1980).
Scangos, G. & Ruddle, F. H. Gene 14, 1–10 (1981).
Hori, T.-A., Shiomi, T. & Sato, K. Proc. natn. Acad. Sci U.S.A. 80, 5655–5659 (1983).
Shows, T. B., McAlpine, P. J. & Miller, R. J. in Proc. 7th Human Gene Mapping Conference (eds Sparkes, R. S., Berg, K., Evans, H. J. & Klinger, H. P.) 340–390 (Karger, Basel, 1984).
Takano, T., Noda, M. & Tamura, T. Nature 296, 269–270 (1982).
Jeffreys, A. J. & Flavell, R. A. Cell 12, 429–439 (1977).
Mulligan, R. E. & Berg, P. Molec. cell. Biol. 1, 449–459 (1981).
Graham, F. & Van der Eb, A. Virology 52, 456–467 (1973).
Grosveld, F. G., Dahl, H. H. M., de Boer, E. & Flavell, R. A. Gene 13, 227–237 (1981).
Grosveld, F. G. et al. Nucleic Acids Res. 10, 6715–6732 (1982).
Groffen, J. et al. Cell 36, 93–99 (1984).
Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Westerveld, A., Hoeijmakers, J., van Duin, M. et al. Molecular cloning of a human DNA repair gene. Nature 310, 425–429 (1984). https://doi.org/10.1038/310425a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/310425a0
