Abstract
The genomic structure of the joining (J) and constant (C) regions of the locus encoding the β-chain of the murine T-cell receptor has been analysed. The gene segments are arranged tandemly (J–C/J–C) within a 15-kilobase region. The two constant-region genes are almost identical, differing by only four amino acids, all in carboxy-terminal portions. Each C region comprises four exons encoding an external globular domain, a small hinge-like region, a transmembrane region and a cytoplasmic tail plus 3′-untranslated region. The two clusters of J regions each contain 7 distinct elements, 12 of which may be functional.
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References
Zinkernagel, R. M. & Doherty, P.C. Adv. Immun. 27, 52–177 (1979).
Sprent, J., Korngold, R. & Molnar-Kimber, K. Springer Semin. Immunopath. 3, 213–245 (1980).
Kappler, J. W., Skidmore, B., White, J. & Marrack, P. J. exp. Med. 153, 1198–1214 (1981).
Hunig, T. R. & Bevan, M. J. J. exp. Med. 155, 111–125 (1982).
Heber-Katz, E. et al. J. exp. Med. 155, 1086–1099 (1982).
Haskins, K. et al. J. exp. Med. 157, 1149–1169 (1983).
Samelson, L. E., Germain, R. N. & Schwartz, R. H. Proc. natn. Acad. Sci. U.S.A. 80, 6972–6976 (1983).
Allison, J. P., MacIntyre, B. W. & Block, D. J. Immun. 129, 2293–2300 (1982).
Meuer, S. et al. J. exp. Med. 157, 705–719 (1983).
McIntyre, B. W. & Allison, J. P. Cell 34, 739–746 (1983).
Kappler, J. W. et al. Cell 35, 295–302 (1983).
Hedrick, S. M., Cohen, D. I., Nielsen, E. A. & Davis, M. M. Nature 308, 149–153 (1984).
Hedrick, S. M., Nielsen, E. A., Kavaler, J., Cohen, D. I. & Davis, M. M. Nature 308, 153–158 (1984).
Honjo, T. A. Rev. Immun. 1, 499–528 (1983).
Chien, Y., Gascoigne, N. R. J., Kavaler, J., Lee, N. E. & Davis, M. M. Nature 309, 322–326 (1984).
Lee, N. E. et al. J. exp. Med. (in the press).
Sakano, H., Huppi, K., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).
Tonegawa, S. et al. Cold Spring Harb. Symp. quant. Biol. 45, 839–858 (1980).
Kabat, E. A., Wu, T. T., Bilofsky, H., Reid-Miller, M. & Perry, H. Sequences of Proteins of Immunological Interest (U.S. Dept of Health and Human Services, Washington DC, 1983).
Zimmer, E. A. thesis, Univ. California, Berkeley (1981).
Ollo, R. & Rougeon, F. Cell 32, 515–523 (1983).
Yanagi, Y. et al. Nature 308, 145–149 (1984).
Honjo, T. et al. Cell 18, 559–568 (1979).
Oi, V.T. et al. Nature 307, 136–140 (1984).
Komaromy, M. et al. Nucleic Acids Res. 11, 6775–6785 (1983).
Manning, M. J. & Turner, R. J. Comparative Immunobiology (Wiley, New York,1976).
Viera, J. & Messing, J. Gene 19, 259–268 (1982).
Maxam, A. & Messing, J. Meth. Enzym. 65, 499–560 (1980).
Acuto, O. et al. Proc. natn. Acad. Sci. U.S.A. 81, 3851–3855 (1984).
Kavater, J., Davis, M. M. & Chien, Yueh-hsiu Nature 310, 421–423 (1984).
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Gascoigne, N., Chien, Yh., Becker, D. et al. Genomic organization and sequence of T-cell receptor β-chain constant- and joining-region genes. Nature 310, 387–391 (1984). https://doi.org/10.1038/310387a0
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DOI: https://doi.org/10.1038/310387a0
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