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Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat

Nature volume 308, pages 467–470 (1984)Cite this article

Abstract

Although the murine retrovirus SL3-3 is highly leukaemo-genic1,2, in both the structure of its genome and in its properties of replication in tissue culture it closely resembles the non-leukaemogenic retrovirus Akv (refs 3, 4). An.earlier investigation of the properties of recombinant SL3-3-Akv viruses localized the major determinant of leukaemogenicity outside the env gene, in a region of the viral genome that includes the gag gene and the noncoding long terminal repeat (LTR)4. To localize the determinant of SL3-3's leukaemogenicity more precisely we have now construced a recombinant provirus containing the LTR of SL3-3 and the coding region of Akv. The leukaemogenicity of these recombinants demonstrates that the determinant of leukaemogenicity ties within the SL3-3 LTR. Nucleotide sequencing of the LTRs of SL3-3 and Akv shows that they differ by a set of changes in the region thought to contain a transcriptional enhancer element. We suggest that enhancer region sequences are the major determinants of leukaemogenicity in these viruses.

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Authors and Affiliations

  1. Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Jack Lenz, Daniel Celander, Robert L. Crowther, Roberto Patarca, Dennis W. Perkins & William A. Haseltine

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  1. Jack Lenz
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  2. Daniel Celander
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  6. William A. Haseltine
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Lenz, J., Celander, D., Crowther, R. et al. Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat. Nature 308, 467–470 (1984). https://doi.org/10.1038/308467a0

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