Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Isolation and structural organization of the human preproenkephalin B gene

Nature volume 306, pages 611–614 (1983)Cite this article

Abstract

The primary structure of porcine preproenkephalin B has been elucidated by cloning and sequencing cDNA1: it contains neoendorphin2,3, dynorphin4,5 and leumorphin1,6,7 (containing rimorphin as its ammo-terminus)8,9. These opioid peptides, each having a leucine-enkephalin structure10, act on the κ-receptor7,11–14. We have now cloned a human genomic DNA segment containing the preproenkephalin B gene. The structural organization of this gene resembles those of the genes encoding the other opioid peptide precursors, that is, preproenkephalin A15 and the corticotropin-β-lipotropin precursor16–21 (ACTH-β-LPH precursor). The primary structure of human preproenkephalin B has been deduced from the gene sequence. The amino acid sequence homology observed between preproenkephalin B and preproenkephalin A, together with the similarity between their gene organizations, suggests that the two genes have been generated from a common ancestor by gene duplication.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Kakidani, H. et al. Nature 298, 245–249 (1982).

    Article  ADS  CAS  Google Scholar 

  2. Minamino, N., Kangawa, K., Chino, N., Sakakibara, S. & Matsuo, H. Biochem. biophys. Res. Commun. 99, 864–870 (1981).

    Article  CAS  Google Scholar 

  3. Kangawa, K., Minamino, N., Chino, N., Sakakibara, S. & Matsuo, H. Biochem. biophys. Res. Commun. 99, 871–878 (1981).

    Article  CAS  Google Scholar 

  4. Goldstein, A., Fischli, W., Lowney, L. I., Hunkapiller, M. & Hood, L. Proc. natn. Acad Sci. U.S.A. 78, 7219–7223 (1981).

    Article  ADS  CAS  Google Scholar 

  5. Tachibana, S., Araki, K., Ohya, S. & Yoshida, S. Nature 295, 339–340 (1982).

    Article  ADS  CAS  Google Scholar 

  6. Yamamoto, Y. et al. Regul. Peptides 6, 163–168 (1983).

    Article  CAS  Google Scholar 

  7. Suda, M. et al. Life Sci. 32, 2769–2775 (1983).

    Article  CAS  Google Scholar 

  8. Kilpatrick, D. L., Wahlstrom, A., Lahm, H. W., Blacher, R. & Udenfriend, S. Proc. natn. Acad. Sci. U.S.A. 79, 6480–6483 (1982).

    Article  ADS  CAS  Google Scholar 

  9. Fischli, W., Goldstein, A., Hunkapiller, M. W. & Hood, L. E. Proc. natn. Acad. Sci. U.S.A 79, 5435–5437 (1982).

    Article  ADS  CAS  Google Scholar 

  10. Hughes, J. et al. Nature 258, 577–579 (1975).

    Article  ADS  CAS  Google Scholar 

  11. Chavkin, C., James, I. F. & Goldstein, A. Science 215, 413–415 (1982).

    Article  ADS  CAS  Google Scholar 

  12. Oka, T. et al. Eur. J. Pharmac. 79, 301–305 (1982).

    Article  CAS  Google Scholar 

  13. Corbett, A. D., Paterson, S. J., McKnight, A. T., Magnan, J. & Kosterlitz, H. W. Nature 299, 79–81 (1982).

    Article  ADS  CAS  Google Scholar 

  14. Paterson, S. J., Robson, L. E. & Kosterlitz, H. W. British med. Bull. 39, 31–36 (1983).

    Article  CAS  Google Scholar 

  15. Noda, M. et al. Nature 297, 431–434 (1982).

    Article  ADS  CAS  Google Scholar 

  16. Nakanishi, S. et al. Nature 287, 752–755 (1980).

    Article  ADS  CAS  Google Scholar 

  17. Nakanishi, S. et al. Eur. J. Biochem. 115, 429–438 (1981).

    Article  CAS  Google Scholar 

  18. Takahashi, H., Teranishi, Y., Nakanishi, S. & Numa, S. FEBS Lett. 135, 97–102 (1981).

    Article  CAS  Google Scholar 

  19. Cochet, M., Chang, A. C. Y. & Cohen, S. N. Nature 297, 335–339 (1982).

    Article  ADS  CAS  Google Scholar 

  20. Whitfeld, P. L., Seeburg, P. H. & Shine, J. DNA 1, 133–143 (1982).

    Article  CAS  Google Scholar 

  21. Notake, M. et al. FEBS Lett. 156, 67–71 (1983).

    Article  CAS  Google Scholar 

  22. Lawn, R. M., Fritsch, E. F., Parker, R. C., Blake, G. & Maniatis, T. Cell 15, 1157–1174 (1978).

    Article  CAS  Google Scholar 

  23. Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).

    Article  ADS  CAS  Google Scholar 

  24. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  25. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  Google Scholar 

  26. Mount, S. M. Nucleic Acids Res. 10, 459–472 (1982).

    Article  CAS  Google Scholar 

  27. Goldberg, M. thesis, Stanford Univ. (1979).

  28. Breathnach, R. & Chambon, P. A. Rev. Biochem. 50, 349–383 (1981).

    Article  CAS  Google Scholar 

  29. Benoist, C., O'Hare, K., Breathnach, R. & Chambon, P. Nucleic Acids Res. 8, 127–142 (1980).

    Article  CAS  Google Scholar 

  30. Gruss, P., Dhar, R. & Khoury, G. Proc. natn. Acad. Sci. U.S.A. 78, 943–947 (1981).

    Article  ADS  CAS  Google Scholar 

  31. Fromm, M. & Berg, P. J. molec. appl. Genet. 1, 457–481 (1982).

    CAS  Google Scholar 

  32. Levinson, B., Khoury, G., Woude, G. V. & Gruss, P. Nature 295, 568–572 (1982).

    Article  ADS  CAS  Google Scholar 

  33. Benoist, C. & Chambon, P. Nature 290, 304–310 (1981).

    Article  ADS  CAS  Google Scholar 

  34. Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 852–862 (1975).

    Article  CAS  Google Scholar 

  35. Steiner, D. F., Quinn, P. S., Chan, S. J., Marsh, J. & Tager, H. S. Ann. N.Y. Acad. Sci 343, 1–16 (1980).

    Article  ADS  CAS  Google Scholar 

  36. Noda, M. et al. Nature 295, 202–206 (1982).

    Article  ADS  CAS  Google Scholar 

  37. Nakanishi, S. et al. Nature 278, 423–427 (1979).

    Article  ADS  CAS  Google Scholar 

  38. Numa, S. & Nakanishi, S. Trends Biochem. Sci. 6, 274–277 (1981).

    Article  CAS  Google Scholar 

  39. Weinstock, R., Sweet, R., Weiss, M., Cedar, H. & Axel, R. Proc. natn. Acad. Sci. U.S.A. 75, 1299–1303 (1978).

    Article  ADS  CAS  Google Scholar 

  40. Okayama, H. & Berg, P. Molec. cell. Biol. 2, 161–170 (1982).

    Article  CAS  Google Scholar 

  41. Ito, H., Ike, Y., Ikuta, S. & Itakura, K. Nucleic Acids Res. 10, 1755–1769 (1982).

    Article  CAS  Google Scholar 

  42. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979).

    Article  CAS  Google Scholar 

  43. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  44. Hanahan, D. & Meselson, M. Gene 10, 63–67 (1980).

    Article  CAS  Google Scholar 

  45. Frischauf, A. M., Garoff, H. & Lehrach, H. Nucleic Acids Res. 8, 5541–5549 (1980).

    Article  CAS  Google Scholar 

  46. Breathnach, R., Benoist, C., O'Hare, K., Gannon, F. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).

    Article  ADS  CAS  Google Scholar 

  47. Dayhoff, M. O., Schwartz, R. M. & Orcutt, B. C. in Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3, 345–352 (National Biomedical Research Foundation, Silver Spring, Maryland, 1978).

    Google Scholar 

  48. Furutani, Y. et al. Nature 301, 537–540 (1983).

    Article  ADS  CAS  Google Scholar 

  49. Comb, M., Seeburg, P. H., Adelman, J., Eiden, L. & Herbert, E. Nature 295, 663–666 (1982).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Chemistry, Kyoto University Faculty of Medicine, Kyoto, 606, Japan

    Saburo Horikawa, Toshiyuki Takai, Mitsuyoshi Toyosato, Hideo Takahashi, Masaharu Noda, Hitoshi Kakidani, Tai Kubo & Shosaku Numa

  2. Pharmaceutical Institute, Keio University School of Medicine, Tokyo, 160, Japan

    Tadaaki Hirose & Seiichi Inayama

  3. Department of Biology, Kyushu University Faculty of Science, Fukuoka, 812, Japan

    Hidenori Hayashida & Takashi Miyata

Authors
  1. Saburo Horikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Toshiyuki Takai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mitsuyoshi Toyosato
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hideo Takahashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Masaharu Noda
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hitoshi Kakidani
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Tai Kubo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Tadaaki Hirose
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Seiichi Inayama
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Hidenori Hayashida
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Takashi Miyata
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Shosaku Numa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Horikawa, S., Takai, T., Toyosato, M. et al. Isolation and structural organization of the human preproenkephalin B gene. Nature 306, 611–614 (1983). https://doi.org/10.1038/306611a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/306611a0

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing