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Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage–ligation reaction

Nature volume 301, pages 578–583 (1983)Cite this article

Abstract

The intervening sequence (IVS) of the Tetrahymena ribosomal RNA precursor is excised as a linear RNA molecule which subsequently cyclizes itself in a protein-independent reaction. Cyclization involves cleavage of the linear IVS RNA 15 nucleotides from its 5′ end and formation of a phosphodiester bond between the new 5′ phosphate and the original 3′-hydroxyl terminus of the IVS. This recombination mechanism is analogous to that by which splicing of the precursor RNA is achieved. The circular molecules appear to have no direct function in RNA splicing, and we propose the cyclization serves to prevent unwanted RNA from driving the splicing reactions backwards.

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References

  1. Abelson, J. A. Rev. Biochem. 48, 1035–1069 (1979).

    Article  CAS  Google Scholar 

  2. Breathnach, R. & Chambon, P. A. Rev. Biochem. 50, 349–383 (1981).

    Article  CAS  Google Scholar 

  3. Cech, T. R., Zaug, A. J., Grabowski, P. J. & Brehm, S. L. The Cell Nucleus Vol. 10 (eds Busch, H. & Rothblum, L.) 171–204 (Academic, New York, 1982).

    Google Scholar 

  4. Peebles, C. L., Ogden, R. C., Knapp, G. & Abelson, J. Cell 18, 27–35 (1979).

    Article  CAS  PubMed  Google Scholar 

  5. Cech, T. R., Zaug, A. J. & Grabowski, P. J. Cell 27, 487–496 (1981).

    Article  CAS  PubMed  Google Scholar 

  6. Kruger, K. et al. Cell 31, 147–157 (1982).

    Article  CAS  PubMed  Google Scholar 

  7. Zaug, A. J. & Cech, T. R. Cell 19, 331–338 (1980).

    Article  CAS  PubMed  Google Scholar 

  8. Carin, M. et al. Nucleic Acids Res. 8, 5551–5566 (1980).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Zaug, A. J. & Cech, T. R. Nucleic Acids Res. 10, 2823–2838 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Grabowski, P. J., Zaug, A. J. & Cech, T. R. Cell 23, 467–476 (1981).

    Article  CAS  PubMed  Google Scholar 

  11. Brehm, S. L. & Cech, T. R. Biochemistry (in the press).

  12. Donis-Keller, H., Maxam, A. M. & Gilbert, W. Nucleic Acids Res. 4, 2527–2538 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Simoncsits, A., Brownlee, G. G., Brown, R. S., Rubin, J. R., & Guilley, H. Nature 269, 833–836 (1977).

    Article  CAS  ADS  PubMed  Google Scholar 

  14. Kan, N. C. & Gall, J. G. Nucleic Acids Res. 10, 2809–2822 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Silber, R., Malathi, V. G. & Hurwitz, H. J. Proc. natn. Acad. Sci. U.S.A. 69, 3009–3013 (1972).

    Article  CAS  ADS  Google Scholar 

  16. Bruce, A. G. & Uhlenbeck, O. C. Nucleic Acids Res. 5, 3665–3677 (1978).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Higgins, N. P. & Cozzarelli, N. R. Meth. Enzym. 68, 50–71 (1979).

    Article  CAS  PubMed  Google Scholar 

  18. Uhlenbeck, O. C. & Gumport, R. I. Enzymes 15, 31–58 (1982).

    Article  CAS  Google Scholar 

  19. Konarska, M., Filipowicz, W., Domdey, H. & Gross, H. L. Nature 293, 112–116 (1981).

    Article  CAS  PubMed  ADS  Google Scholar 

  20. Konarska, M., Filipowicz, W. & Gross, H. J. Proc. natn. Acad. Sci. U.S.A. 79, 1474–1478 (1982).

    Article  CAS  ADS  Google Scholar 

  21. Gellert, M. A. Rev. Biochem. 50, 879–910 (1981).

    Article  CAS  Google Scholar 

  22. Eisenberg, S., Scott, J. F. & Kornberg, A. Cold Spring Harb. Symp. quant. Biol. 43, 295–302 (1978).

    Article  Google Scholar 

  23. Sumida-Yasumoto, C. et al. Cold Spring Harb. Symp. quant. Biol. 43, 311–329 (1978).

    Article  Google Scholar 

  24. van der Ende, A., Langeveld, S. A., Teertstra, R., van Arkel, G. A. & Weisbeek, P. J. Nucleic Acids Res. 9, 2037–2053 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Tinoco, I. Jr et al. Nature new Biol. 246, 40–41 (1973).

    Article  CAS  PubMed  Google Scholar 

  26. Wild, M. A. & Sommer, R. Nature 283, 693–694 (1980).

    Article  CAS  ADS  PubMed  Google Scholar 

  27. Fox, G. & Woese, C. R. Nature 256, 505–507 (1975).

    Article  CAS  ADS  PubMed  Google Scholar 

  28. Noller, H. F. & Woese, C. R. Science 212, 403–410 (1981).

    Article  CAS  PubMed  ADS  Google Scholar 

  29. Wild, M. A. & Gall, J. G. Cell 16, 565–573 (1979).

    Article  CAS  PubMed  Google Scholar 

  30. Din, N. & Engberg, J. J. molec. Biol. 134, 555–574 (1979).

    Article  CAS  PubMed  Google Scholar 

  31. Wild, M. A. thesis, Yale Univ. (1980).

  32. Halbreich, A., Pajot, P., Foucher, M., Grandchamp, C. & Slonimski, P. Cell 19, 321–329 (1980).

    Article  CAS  PubMed  Google Scholar 

  33. Arnberg, A. C., VanOmmen, G.-J. B., Grivell, L. A., VanBruggen, E. F. J. & Borst, P. Cell 19, 313–319 (1980).

    Article  CAS  PubMed  Google Scholar 

  34. Knapp, G., Ogden, R. C., Peebles, C. L. & Abelson, J. Cell 18, 37–45 (1979).

    Article  CAS  PubMed  Google Scholar 

  35. Gross, H. J. et al. Nature 273, 203–208 (1978).

    Article  CAS  ADS  PubMed  Google Scholar 

  36. Branch, A. D. et al. Science 217, 1147–1149 (1982).

    Article  CAS  PubMed  ADS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, University of Colorado, Boulder, Colorado, 80309, USA

    Arthur J. Zaug, Paula J. Grabowski & Thomas R. Cech

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  1. Arthur J. Zaug
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  2. Paula J. Grabowski
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  3. Thomas R. Cech
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Zaug, A., Grabowski, P. & Cech, T. Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage–ligation reaction. Nature 301, 578–583 (1983). https://doi.org/10.1038/301578a0

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