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The sequences of an expressed rat α-tubulin gene and a pseudogene with an inserted repetitive element

Nature volume 300, pages 330–335 (1982)Cite this article

Abstract

The rat genome contains two segments closely related to a rat α-tubulin mRNA. Both have been cloned and complete nucleotide sequences are presented. Analysis of the structure and sequence of one of these establishes it as a functional α-tubulin gene. The second segment is a processed α-tubulin pseudogene. Comparison of this pseudogene to the mRNA and gene coding for α-tubulin strongly suggests that a mature mRNA was involved in its origin. Features of the pseudogene and a dispersed repetitive element inserted within it possibly reflect a common RNA-mediated process of insertion.

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References

  1. Cleveland, D. W. et al. Cell 20, 95–105 (1980).

    Article  CAS  Google Scholar 

  2. Ponstingl, H., Krauhs, E., Little, M. & Kempf, T. Proc. natn. Acad. Sci. U.S.A. 78, 2757–2761 (1981).

    Article  ADS  CAS  Google Scholar 

  3. Ginzburg, I., Behar, L., Givol, D. & Littauer, U. Z. Nucleic Acids Res. 9, 2691–2697 (1981).

    Article  CAS  Google Scholar 

  4. Lemischka, I. R., Farmer, S., Racaniello, V. R. & Sharp, P. A. J. molec. Biol. 150, 101–120 (1981).

    Article  Google Scholar 

  5. Wilde, C. D., Chow, L. T., Wefald, F. C. & Cowan, N. J. Proc. natn. Acad. Sci. U.S.A. 79, 96–100 (1982).

    Article  ADS  CAS  Google Scholar 

  6. Cowan, N. J., Wilde, C. D., Chow, L. T. & Wefald, F. C. Proc. natn. Acad. Sci. U.S.A. 78, 4877–4881 (1981).

    Article  ADS  CAS  Google Scholar 

  7. Wilde, C. D., Crowther, C. E. & Cowan, N. J. J. molec. Giol. 155, 533–538 (1982).

    Article  CAS  Google Scholar 

  8. Hollis, G. F., Hieter, P. A., McBride, D. W., Swan, D. & Leder, P. Nature 296, 321–325 (1982).

    Article  ADS  CAS  Google Scholar 

  9. Van Arsdell, S. W. Cell 26, 11–17 (1981).

    Article  CAS  Google Scholar 

  10. Schmid, C. W. & Jelinek, W. R. Science 216, 1065–1070 (1982).

    Article  ADS  CAS  Google Scholar 

  11. Kalfayan, L. & Wensink, P. C. Cell 24, 97–106 (1981).

    Article  CAS  Google Scholar 

  12. Sanchez, F., Natzle, J. E., Cleveland, D. W., Kirschner, M. W. & McCarthy, B. J. Cell 22, 845–854 (1980).

    Article  CAS  Google Scholar 

  13. Alexandraki, D. & Ruderman, J. Molec. Cell. Biol. 1, 1125–1137 (1981).

    Article  CAS  Google Scholar 

  14. Mischke, D. & Pardue, M. L. J. molec. Biol. 156, 449–466 (1982).

    Article  CAS  Google Scholar 

  15. Benton, W. D. & Davis, R. W. Science 196, 180–181 (1977).

    Article  ADS  CAS  Google Scholar 

  16. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–580 (1980).

    Article  CAS  Google Scholar 

  17. Sharp, P. A. Cell 23, 643–646 (1981).

    Article  CAS  Google Scholar 

  18. Goldberg, M. thesis, Stanford Univ. (1979).

  19. Manley, J. L., Fire, A., Cano, A., Sharp, P. A. & Gefter, M. Proc. natn. Acad. Sci. U.S.A. 77, 3855–2859 (1980).

    Article  ADS  CAS  Google Scholar 

  20. Galli, G., Hofstetter, H. & Birnstiel, M. L. Nature 294, 626–631 (1981).

    Article  ADS  CAS  Google Scholar 

  21. Barta, A., Richards, R., Baxter, J. D. & Shine, J. Proc. natn. Acad. Sci. U.S.A. 78, 4867–4871 (1981).

    Article  ADS  CAS  Google Scholar 

  22. Zakut, R. et al. Nature 298, 857–859 (1982).

    Article  ADS  CAS  Google Scholar 

  23. Chambon, P. & Breathnach, R. A. Rev. Biochem. 50, 349–383 (1981).

    Article  Google Scholar 

  24. Proudfoot, N. J. & Browlee, G. G. Nature 263, 211–214 (1976).

    Article  ADS  CAS  Google Scholar 

  25. Ben-Ze'ev, Farmer, S. R. & Penman, S. Cell 17, 319–325 (1979).

    Article  CAS  Google Scholar 

  26. Cleveland, D. W., Lopata, M. A., Sherline, P. & Kirschner, M. W. Cell 25, 537–546 (1981).

    Article  CAS  Google Scholar 

  27. Perler, F. et al. Cell 20, 555–566 (1980).

    Article  CAS  Google Scholar 

  28. Wilson, A. C., Carlson, S. S. & White, T. J. A. Rev. Biochem. 46, 573–639 (1977).

    Article  CAS  Google Scholar 

  29. Nishioka, Y., Leder, A. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 77, 2806–2809 (1980).

    Article  ADS  CAS  Google Scholar 

  30. Wilde, C. D., Crowther, C. E., Cripe, T. P., Gwo-Shu Lee, M. & Cowan, N. J. Nature 297, 83–84 (1982).

    Article  ADS  CAS  Google Scholar 

  31. Wilde, C. D., Crowther, C. E. & Cowan, N. J. Science (in the press).

  32. Sutcliffe, J. G., Milner, R. J., Bloom, F. E. & Lerner, R. A. Proc. natn. Acad. Sci. U.S.A. 79, 4942–4946 (1982).

    Article  ADS  CAS  Google Scholar 

  33. Elder, J. T., Pan, J., Duncan, C. H. & Weissman, S. H. Nucleic Acids Res. 9, 1171–1189 (1981).

    Article  CAS  Google Scholar 

  34. Grimaldi, G. & Singer, M. Proc. natn. Acad. Sci. U.S.A. 79, 1497–1500 (1982).

    Article  ADS  CAS  Google Scholar 

  35. Calos, M. & Miller, J. H. Cell 20, 579–595 (1980).

    Article  CAS  Google Scholar 

  36. Stringer, J. R. Nature 296, 363–364 (1982).

    Article  ADS  CAS  Google Scholar 

  37. Steffen, D. & Weinberg, R. A. Cell 15, 1003–1010 (1978).

    Article  CAS  Google Scholar 

  38. Southern, E. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  39. Vogelstein, B. & Gillespie, D. Proc. natn. Acad. Sci. U.S.A. 76, 615–619 (1979).

    Article  ADS  CAS  Google Scholar 

  40. Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

    Article  CAS  Google Scholar 

  41. Davis, R. W., Simon, M. & Davidson, N. Meth. Enzym. 21, 413–428 (1976).

    Article  Google Scholar 

  42. Li, W. Y., Reddy, R., Henning, P. & Busch, H. J. biol. Chem. 257, 5136–5142 (1982).

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Ihor Lemischka & Phillip A. Sharp

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  1. Ihor Lemischka
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  2. Phillip A. Sharp
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Lemischka, I., Sharp, P. The sequences of an expressed rat α-tubulin gene and a pseudogene with an inserted repetitive element. Nature 300, 330–335 (1982). https://doi.org/10.1038/300330a0

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