Abstract
The sequence of a cDNA encoding the nonapeptide arginine vasopressin (A VP) and its carrier protein, neurophysin II (NpII) from bovine hypothalamus, proves that the 166-amino acid precursor molecule contains a signal peptide of 19 amino acids followed directly by A VP connected to NpII by a Gly-Lys-Arg sequence. The carboxy-terminal region of the precursor contains a naturally occurring glycopolypeptide of 39 amino acids which is separated from NpII by a single arginine residue.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Walter, R. Ann. N.Y. Acad. Sci. 248, 1–512 (1975).
Pickering, B. T. & Jones, C. W. in Hormonal Proteins and Peptides Vol 5 (ed. Li, C. H) 103–158 (Academic, New York, 1978).
Breslow, E. A. Rev. Biochem. 48, 251–274 (1979).
Brownstein, M. J., Russell, J. T. & Gainer, H. Science 207, 373–378 (1980).
Sokol, H. W., Zimmermann, E. A., Sawyer, W. H. & Robinson, A. G. Endocrinology 98, 1176–1188 (1976).
Zimmermann, E. A. & Defendini, R. in Neurohypophysis (eds Moses, A. M. & Share, L.) 22–29 (Karger, Basel, 1977).
Sachs, H. & Takabatake, Y. Endocrinology 75, 943–948 (1964).
Sachs, H., Fawcett, P., Takabatake, Y. & Portanova, R. Recent Prog. Horm. Res. 25, 447–491 (1969).
Valtin, H., Sawyer, W. H. & Sokol, H. W. Endocrinology 77, 701–706 (1965).
Friesen, H. G. & Astwood, E. B. Endocrinology 80, 278–287 (1967).
Burford, G. D., Jones, C. W. & Pickering, B. T. Biochem. J. 124, 809–813 (1971).
Pickering, B. T. et al. Ann. N.Y. Acad. Sci. 248, 15–35 (1975).
Sunde, D. A. & Sokol, H. W. Ann. N.Y. Acad. Sci. 248, 345–364 (1975).
Richter, D., Ivell, R. & Schmale, H. in Biosynthesis, Modification and Processing of Cellular and Viral Polyproteins (eds Koch, G. & Richter, D.) 5–13 (Academic, New York, 1980).
Brownstein, M. J. & Gainer, H. Proc. natn. Acad. Sci. U.S.A. 74, 4046–4049 (1977).
Russell, J. T., Brownstein, M. J. & Gainer, H. Proc. natn. Acad. Sci. U.S.A. 76, 6086–6090 (1979).
Lauber, M., Camier, M. & Cohen, P. FEBS Lett. 97, 343–347 (1979).
Brownstein, M. J., Robinson, A. G. & Gainer, H. Nature 269, 259–261 (1977).
Giudice, L. C. & Chaiken, J. M. Proc. natn. Acad. Sci. U.S.A. 76, 3800–3804 (1979).
Lin, C., Joseph-Bravo, P., Sherman, T., Chan, L. & McKelvy, J. F. Biochem. biophys. Res. Commun. 89, 943–950 (1979).
Schmale, H., Leipold, B. & Richter, D. FEBS Lett. 108, 311–316 (1979).
Schmale, H. & Richter, D. FEBS Lett. 121, 358–362 (1980).
Schmale, H. & Richter, D. Proc. natn. Acad. Sci. U.S.A. 78, 766–769 (1981).
Schmale, H. & Richter, D. Neuropeptides 2, 47–52 (1981).
Richter, D., Schmale, H., Ivell, R. & Kalthoff, H. in Hormonally Active Brain Peptides (ed. McKerns, K. W.) (Plenum, New York, in the press).
Land, H., Grez, M., Hausser, H., Lindenmaier, W. & Schütz, G. Nucleic Acids Res. 9, 2251–2266 (1981).
Richter, D., Schmale, H., Ivell, R. & Schmidt, C. in Biosynthesis, Modification and Processing of Cellular and Viral Polyproteins (eds Koch, G. & Richter, D.) 43–66 (Academic, New York, 1980).
Ricciardi, R. P., Miller, J. S. & Roberts, B. E. Proc. natn. Acad. Sci. U.S.A. 76, 4928–4931 (1979).
Leung, D. W., Lui, A. C. P., Meriless, H., McBride, B. C. & Smith, M. Nucleic Acids Res. 6, 17–25 (1979).
Sussenbach, J. S., Steenbergh, P. H., Rost, J. A., Van Leeuwen, W. J. & Van Embden, J. D. A. Nucleic Acids Res. 5, 1153–1163 (1978).
Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Osterburg, G., Glatting, K. H. & Sommer, R. Nucleic Acids Res. (in the press).
Chauvet, M. T., Chauvet, J. & Acher, R. Eur. J. Biochem. 69, 475–485 (1976).
Ivell, R., Schmale, H. & Richter, D. Biochem. biophys. Res. Commun. 102, 1230–1236 (1981).
Smyth, D. G. & Massey, D. E. Biochem. biophys. Res. Commun. 87, 1006–1010 (1979).
Lingappa, V. R. & Blobel, G. Recent Prog. Horm. Res. 36, 451–475 (1980).
Acher, R. Angew. Chem. 91, 905–919 (1979).
Nicolas, P. et al. Proc. natn. Acad. Sci. U.S.A. 77, 2587–2591 (1980).
Schreier, P. H. & Cortese, R. J. molec. Biol. 129, 169–172 (1979).
Efstratiadis, A., Kafatos, F. C. & Maniatis, T. Cell 10, 571–585 (1977).
Ullrich, A. et al. Science 196, 1313–1318 (1977).
Seeburg, P. H., Shine, J., Martial, J. A., Baxter, J. D. & Goodman, H. M. Nature 270, 486–494 (1977).
Nakanishi, S. et al. Nature 278, 423–427 (1979).
Szybalski, W. Meth. Enzym. 12B, 330–360 (1968).
Swartz, M. N., Trautner, T. A. & Kornberg, A. J. biol. Chem. 237, 1961–1967 (1962).
Stern, A. S., Jones, B. N., Shirely, J. E., Stein, S. & Udenfriend, S. Proc. natn. Acad. Sci. U.S.A. 78, 1962–1966 (1981).
Hobart, P., Crawford, R., Shen, L. P., Pictet, R. & Rutter, W. J. Nature 288, 137–141 (1980).
Jacobs, J. W. et al. Science 213, 457–459 (1981).
Suchanek, G. & Kreil, G. Proc. natn. Acad. Sci. U.S.A. 74, 975–978 (1977).
Amara, S. G., David, D. N., Rosenfeld, M. G., Roos, B. A. & Evans, R. M. Proc. natn. Acad. Sci. U.S.A. 77, 4444–4448 (1980).
Shibasaki, T., Ling, N. & Guillemin, R. Biochem. biophys. Res. Commun. 96, 1393–1399 (1980).
Seidah, N. G., Rochemont, J., Hamelin, J., Benjannet, S. & Chrétien, M. Biochem. biophys. Res. Commun. 102, 710–716 (1981).
Pless, D. D. & Lennarz, W. J. Proc. natn. Acad. Sci. U.S.A. 74, 134–138 (1977).
Seidah, N. G., Benjannet, S. & Chrétien, M. Biochem. biophys. Res. Commun. 100, 901–907 (1981).
Holwerda, D. A. Eur. J. Biochem. 28, 340–346 (1972).
Hunt, L. T., Ledley, F. D. & Dayhoff, M. O. in Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3 (ed. Dayhoff, M. O.) 145–164 (National Biomedical Research Foundation, Washington DC, 1979).
Capra, J. D., Kehoe, J. M.., Kotelchuck, D., Walter, R. & Breslow, E. Proc. natn. Acad. Sci. U.S.A. 69, 431–434 (1972).
Legros, J. J., Louis, F., Grötschel-Stewart, U. & Tranchimont, P. Ann. N.Y. Acad. Sci. 248, 157–171 (1975).
Henninghausen, L. & Sippel, A. E. (in preparation).
Robinson, A. G. Ann. N.Y. Acad. Sci. 248, 246–256 (1975).
Pearson, D. B., Goodman, R. & Sachs, H. Science 187, 1081–1082 (1975).
Gillespie, D. & Vogelstein, B. Proc. natn. Acad. Sci. U.S.A. 76, 615–619 (1979).
Roewekamp, W. & Firtel, R. A. Devl Biol. 79, 409–418 (1980).
Hubatek, J. & Glover, S. W. J. molec. Biol. 50, 111–127 (1970).
Dagert, M. & Ehrilch, S. D. Gene 6, 23–28 (1979).
Klein, R. D., Selsing, E. & Wells, R. D. Plasmid 3, 88–91 (1980).
Kafatos, F. C., Jones, C. W. & Efstratiadis, A. Nucleic Acids Res. 7, 1542–1552 (1979).
Maizels, N. Cell 9, 431–438 (1976).
Dobner, P. R., Kawasaki, E. S., Yu, L. Y. & Bancroft, F. C. Proc. natn. Acad. Sci. U.S.A. 78, 2230–2234 (1981).
Lindenmaier, W. et al. Nucleic Acids Res. 5, 1221–1232 (1979).
Sippel, A. E. et al. Nucleic Acids Res. 5, 3275–3294 (1978).
McReynolds, L. et al. Nature 273, 723–728 (1978).
Catterall, J. F., Stein, J. P., Kristo, P., Means, A. R. & O'Malley, B. W. J. Cell Biol. 87, 480–487 (1980).
Jung, A., Sippel, A. E., Grez, M. & Schütz, G. Proc. natn. Acad. Sci. U.S.A. 77, 5759–5763 (1980).
Sutcliffe, J. G. Nucleic Acids Res. 5, 2721–2728 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Land, H., Schütz, G., Schmale, H. et al. Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin–neurophysin II precursor. Nature 295, 299–303 (1982). https://doi.org/10.1038/295299a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/295299a0