Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin–neurophysin II precursor

Nature volume 295, pages 299–303 (1982)Cite this article

Abstract

The sequence of a cDNA encoding the nonapeptide arginine vasopressin (A VP) and its carrier protein, neurophysin II (NpII) from bovine hypothalamus, proves that the 166-amino acid precursor molecule contains a signal peptide of 19 amino acids followed directly by A VP connected to NpII by a Gly-Lys-Arg sequence. The carboxy-terminal region of the precursor contains a naturally occurring glycopolypeptide of 39 amino acids which is separated from NpII by a single arginine residue.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Walter, R. Ann. N.Y. Acad. Sci. 248, 1–512 (1975).

    Article  ADS  Google Scholar 

  2. Pickering, B. T. & Jones, C. W. in Hormonal Proteins and Peptides Vol 5 (ed. Li, C. H) 103–158 (Academic, New York, 1978).

    Google Scholar 

  3. Breslow, E. A. Rev. Biochem. 48, 251–274 (1979).

    Article  CAS  Google Scholar 

  4. Brownstein, M. J., Russell, J. T. & Gainer, H. Science 207, 373–378 (1980).

    Article  ADS  CAS  Google Scholar 

  5. Sokol, H. W., Zimmermann, E. A., Sawyer, W. H. & Robinson, A. G. Endocrinology 98, 1176–1188 (1976).

    Article  CAS  Google Scholar 

  6. Zimmermann, E. A. & Defendini, R. in Neurohypophysis (eds Moses, A. M. & Share, L.) 22–29 (Karger, Basel, 1977).

    Google Scholar 

  7. Sachs, H. & Takabatake, Y. Endocrinology 75, 943–948 (1964).

    Article  CAS  Google Scholar 

  8. Sachs, H., Fawcett, P., Takabatake, Y. & Portanova, R. Recent Prog. Horm. Res. 25, 447–491 (1969).

    CAS  PubMed  Google Scholar 

  9. Valtin, H., Sawyer, W. H. & Sokol, H. W. Endocrinology 77, 701–706 (1965).

    Article  CAS  Google Scholar 

  10. Friesen, H. G. & Astwood, E. B. Endocrinology 80, 278–287 (1967).

    Article  CAS  Google Scholar 

  11. Burford, G. D., Jones, C. W. & Pickering, B. T. Biochem. J. 124, 809–813 (1971).

    Article  CAS  Google Scholar 

  12. Pickering, B. T. et al. Ann. N.Y. Acad. Sci. 248, 15–35 (1975).

    Article  ADS  CAS  Google Scholar 

  13. Sunde, D. A. & Sokol, H. W. Ann. N.Y. Acad. Sci. 248, 345–364 (1975).

    Article  ADS  CAS  Google Scholar 

  14. Richter, D., Ivell, R. & Schmale, H. in Biosynthesis, Modification and Processing of Cellular and Viral Polyproteins (eds Koch, G. & Richter, D.) 5–13 (Academic, New York, 1980).

    Book  Google Scholar 

  15. Brownstein, M. J. & Gainer, H. Proc. natn. Acad. Sci. U.S.A. 74, 4046–4049 (1977).

    Article  ADS  CAS  Google Scholar 

  16. Russell, J. T., Brownstein, M. J. & Gainer, H. Proc. natn. Acad. Sci. U.S.A. 76, 6086–6090 (1979).

    Article  ADS  CAS  Google Scholar 

  17. Lauber, M., Camier, M. & Cohen, P. FEBS Lett. 97, 343–347 (1979).

    Article  CAS  Google Scholar 

  18. Brownstein, M. J., Robinson, A. G. & Gainer, H. Nature 269, 259–261 (1977).

    Article  ADS  CAS  Google Scholar 

  19. Giudice, L. C. & Chaiken, J. M. Proc. natn. Acad. Sci. U.S.A. 76, 3800–3804 (1979).

    Article  ADS  CAS  Google Scholar 

  20. Lin, C., Joseph-Bravo, P., Sherman, T., Chan, L. & McKelvy, J. F. Biochem. biophys. Res. Commun. 89, 943–950 (1979).

    Article  CAS  Google Scholar 

  21. Schmale, H., Leipold, B. & Richter, D. FEBS Lett. 108, 311–316 (1979).

    Article  CAS  Google Scholar 

  22. Schmale, H. & Richter, D. FEBS Lett. 121, 358–362 (1980).

    Article  CAS  Google Scholar 

  23. Schmale, H. & Richter, D. Proc. natn. Acad. Sci. U.S.A. 78, 766–769 (1981).

    Article  ADS  CAS  Google Scholar 

  24. Schmale, H. & Richter, D. Neuropeptides 2, 47–52 (1981).

    Article  CAS  Google Scholar 

  25. Richter, D., Schmale, H., Ivell, R. & Kalthoff, H. in Hormonally Active Brain Peptides (ed. McKerns, K. W.) (Plenum, New York, in the press).

  26. Land, H., Grez, M., Hausser, H., Lindenmaier, W. & Schütz, G. Nucleic Acids Res. 9, 2251–2266 (1981).

    Article  CAS  Google Scholar 

  27. Richter, D., Schmale, H., Ivell, R. & Schmidt, C. in Biosynthesis, Modification and Processing of Cellular and Viral Polyproteins (eds Koch, G. & Richter, D.) 43–66 (Academic, New York, 1980).

    Book  Google Scholar 

  28. Ricciardi, R. P., Miller, J. S. & Roberts, B. E. Proc. natn. Acad. Sci. U.S.A. 76, 4928–4931 (1979).

    Article  ADS  Google Scholar 

  29. Leung, D. W., Lui, A. C. P., Meriless, H., McBride, B. C. & Smith, M. Nucleic Acids Res. 6, 17–25 (1979).

    Article  CAS  Google Scholar 

  30. Sussenbach, J. S., Steenbergh, P. H., Rost, J. A., Van Leeuwen, W. J. & Van Embden, J. D. A. Nucleic Acids Res. 5, 1153–1163 (1978).

    Article  CAS  Google Scholar 

  31. Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  32. Osterburg, G., Glatting, K. H. & Sommer, R. Nucleic Acids Res. (in the press).

  33. Chauvet, M. T., Chauvet, J. & Acher, R. Eur. J. Biochem. 69, 475–485 (1976).

    Article  CAS  Google Scholar 

  34. Ivell, R., Schmale, H. & Richter, D. Biochem. biophys. Res. Commun. 102, 1230–1236 (1981).

    Article  CAS  Google Scholar 

  35. Smyth, D. G. & Massey, D. E. Biochem. biophys. Res. Commun. 87, 1006–1010 (1979).

    Article  CAS  Google Scholar 

  36. Lingappa, V. R. & Blobel, G. Recent Prog. Horm. Res. 36, 451–475 (1980).

    CAS  PubMed  Google Scholar 

  37. Acher, R. Angew. Chem. 91, 905–919 (1979).

    Article  CAS  Google Scholar 

  38. Nicolas, P. et al. Proc. natn. Acad. Sci. U.S.A. 77, 2587–2591 (1980).

    Article  ADS  CAS  Google Scholar 

  39. Schreier, P. H. & Cortese, R. J. molec. Biol. 129, 169–172 (1979).

    Article  CAS  Google Scholar 

  40. Efstratiadis, A., Kafatos, F. C. & Maniatis, T. Cell 10, 571–585 (1977).

    Article  CAS  Google Scholar 

  41. Ullrich, A. et al. Science 196, 1313–1318 (1977).

    Article  ADS  CAS  Google Scholar 

  42. Seeburg, P. H., Shine, J., Martial, J. A., Baxter, J. D. & Goodman, H. M. Nature 270, 486–494 (1977).

    Article  ADS  CAS  Google Scholar 

  43. Nakanishi, S. et al. Nature 278, 423–427 (1979).

    Article  ADS  CAS  Google Scholar 

  44. Szybalski, W. Meth. Enzym. 12B, 330–360 (1968).

    Article  CAS  Google Scholar 

  45. Swartz, M. N., Trautner, T. A. & Kornberg, A. J. biol. Chem. 237, 1961–1967 (1962).

    CAS  PubMed  Google Scholar 

  46. Stern, A. S., Jones, B. N., Shirely, J. E., Stein, S. & Udenfriend, S. Proc. natn. Acad. Sci. U.S.A. 78, 1962–1966 (1981).

    Article  ADS  CAS  Google Scholar 

  47. Hobart, P., Crawford, R., Shen, L. P., Pictet, R. & Rutter, W. J. Nature 288, 137–141 (1980).

    Article  ADS  CAS  Google Scholar 

  48. Jacobs, J. W. et al. Science 213, 457–459 (1981).

    Article  ADS  CAS  Google Scholar 

  49. Suchanek, G. & Kreil, G. Proc. natn. Acad. Sci. U.S.A. 74, 975–978 (1977).

    Article  ADS  CAS  Google Scholar 

  50. Amara, S. G., David, D. N., Rosenfeld, M. G., Roos, B. A. & Evans, R. M. Proc. natn. Acad. Sci. U.S.A. 77, 4444–4448 (1980).

    Article  ADS  CAS  Google Scholar 

  51. Shibasaki, T., Ling, N. & Guillemin, R. Biochem. biophys. Res. Commun. 96, 1393–1399 (1980).

    Article  CAS  Google Scholar 

  52. Seidah, N. G., Rochemont, J., Hamelin, J., Benjannet, S. & Chrétien, M. Biochem. biophys. Res. Commun. 102, 710–716 (1981).

    Article  CAS  Google Scholar 

  53. Pless, D. D. & Lennarz, W. J. Proc. natn. Acad. Sci. U.S.A. 74, 134–138 (1977).

    Article  ADS  CAS  Google Scholar 

  54. Seidah, N. G., Benjannet, S. & Chrétien, M. Biochem. biophys. Res. Commun. 100, 901–907 (1981).

    Article  CAS  Google Scholar 

  55. Holwerda, D. A. Eur. J. Biochem. 28, 340–346 (1972).

    Article  CAS  Google Scholar 

  56. Hunt, L. T., Ledley, F. D. & Dayhoff, M. O. in Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3 (ed. Dayhoff, M. O.) 145–164 (National Biomedical Research Foundation, Washington DC, 1979).

    Google Scholar 

  57. Capra, J. D., Kehoe, J. M.., Kotelchuck, D., Walter, R. & Breslow, E. Proc. natn. Acad. Sci. U.S.A. 69, 431–434 (1972).

    Article  ADS  CAS  Google Scholar 

  58. Legros, J. J., Louis, F., Grötschel-Stewart, U. & Tranchimont, P. Ann. N.Y. Acad. Sci. 248, 157–171 (1975).

    Article  ADS  CAS  Google Scholar 

  59. Henninghausen, L. & Sippel, A. E. (in preparation).

  60. Robinson, A. G. Ann. N.Y. Acad. Sci. 248, 246–256 (1975).

    Article  ADS  CAS  Google Scholar 

  61. Pearson, D. B., Goodman, R. & Sachs, H. Science 187, 1081–1082 (1975).

    Article  ADS  CAS  Google Scholar 

  62. Gillespie, D. & Vogelstein, B. Proc. natn. Acad. Sci. U.S.A. 76, 615–619 (1979).

    Article  ADS  Google Scholar 

  63. Roewekamp, W. & Firtel, R. A. Devl Biol. 79, 409–418 (1980).

    Article  Google Scholar 

  64. Hubatek, J. & Glover, S. W. J. molec. Biol. 50, 111–127 (1970).

    Article  Google Scholar 

  65. Dagert, M. & Ehrilch, S. D. Gene 6, 23–28 (1979).

    Article  CAS  Google Scholar 

  66. Klein, R. D., Selsing, E. & Wells, R. D. Plasmid 3, 88–91 (1980).

    Article  CAS  Google Scholar 

  67. Kafatos, F. C., Jones, C. W. & Efstratiadis, A. Nucleic Acids Res. 7, 1542–1552 (1979).

    Article  Google Scholar 

  68. Maizels, N. Cell 9, 431–438 (1976).

    Article  CAS  Google Scholar 

  69. Dobner, P. R., Kawasaki, E. S., Yu, L. Y. & Bancroft, F. C. Proc. natn. Acad. Sci. U.S.A. 78, 2230–2234 (1981).

    Article  ADS  CAS  Google Scholar 

  70. Lindenmaier, W. et al. Nucleic Acids Res. 5, 1221–1232 (1979).

    Article  Google Scholar 

  71. Sippel, A. E. et al. Nucleic Acids Res. 5, 3275–3294 (1978).

    Article  CAS  Google Scholar 

  72. McReynolds, L. et al. Nature 273, 723–728 (1978).

    Article  ADS  CAS  Google Scholar 

  73. Catterall, J. F., Stein, J. P., Kristo, P., Means, A. R. & O'Malley, B. W. J. Cell Biol. 87, 480–487 (1980).

    Article  CAS  Google Scholar 

  74. Jung, A., Sippel, A. E., Grez, M. & Schütz, G. Proc. natn. Acad. Sci. U.S.A. 77, 5759–5763 (1980).

    Article  ADS  CAS  Google Scholar 

  75. Sutcliffe, J. G. Nucleic Acids Res. 5, 2721–2728 (1978).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Zell- und Tumorbiologie, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-6900, Heidelberg, FRG

    Hartmut Land & Günther Schütz

  2. Institut für Physiologische Chemie, Abteilung Zellbiochemie, Universität Hamburg, Martinistrasse 52, D-2000, Hamburg, 20, FRG

    Hartwig Schmale & Dietmar Richter

Authors
  1. Hartmut Land
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Günther Schütz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hartwig Schmale
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dietmar Richter
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Land, H., Schütz, G., Schmale, H. et al. Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin–neurophysin II precursor. Nature 295, 299–303 (1982). https://doi.org/10.1038/295299a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/295299a0

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing