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Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 Å resolution

Nature volume 289, pages 366–373 (1981)Cite this article

Abstract

The haemagglutinin glycoprotein of influenza virus is a trimer comprising two structurally distinct regions: a triple-stranded coiled-coil of α-helices extends 76 Å from the membrane and a globular region of antiparallel β-sheet, which contains the receptor binding site and the variable antigenic determinants, is positioned on top of this stem. Each subunit has an unusual loop-like topology, starting at the membrane, extending 135 Å distally and folding back to enter the membrane.

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References

  1. Laver, W. G. & Kilbourne, E. D. Virology 30, 493–501 (1966).

    Article  CAS  Google Scholar 

  2. Brand, C. M. & Skehel, J. J. Nature new Biol. 238, 145–147 (1972).

    Article  CAS  Google Scholar 

  3. Wiley, D. C. & Skehel, J. J. Topics infect. Dis. 3, 135–138 (1978).

    Article  CAS  Google Scholar 

  4. Skehel, J. J. & Waterfield, M. D. Proc. natn. Acad. Sci. U.S.A. 72, 93–97 (1975).

    Article  CAS  ADS  Google Scholar 

  5. Porter, A. G. et al. Nature 282, 471–477 (1979).

    Article  CAS  ADS  Google Scholar 

  6. Ward, C. W. & Dopheide, T. A. in Structure and Variation in Influenza Virus (eds Laver, W. G. & Air, G. M.) 27–38 (Elsevier, Amsterdam, 1980).

    Google Scholar 

  7. Dopheide, T. A. & Ward, C. W. in Structure and Variation in Influenza Virus (eds Laver, W. G. & Air, G. M.) 21–26 (Elsevier, Amsterdam, 1980).

    Google Scholar 

  8. Sleigh, M. J., Both, G. W., Brownlee, G. G., Bender, V. J. & Moss, B. A. in Structure and Variation in Influenza Virus (eds Laver, W. G. & Air, G. M.) 69–78 (Elsevier, Amsterdam, 1980).

    Google Scholar 

  9. Gething, M.J., Skehel, J. & Waterfield, M. D. in Structure and Variation in Influenza Virus (eds Laver, W. G. & Air, G. M.) 1–10 (Elsevier, Amsterdam, 1980).

    Google Scholar 

  10. Min Jou, W. et al. in Structure and Variation in Influenza Virus (eds Laver, W. G. & Air, G. M.) 63–68 (Elsevier, Amsterdam, 1980).

    Google Scholar 

  11. Verhoeyen, M. et al. Nature 286, 771–776 (1980).

    Article  CAS  ADS  Google Scholar 

  12. Threlfall, G., Barber, C., Carey, N. & Emtage, S. in Structure and Variation in Influenza Virus (eds Laver, W. G. & Air, G. M.) 51–61 (Elsevier, Amsterdam, 1980).

    Google Scholar 

  13. Min Jou, W. et al. Cell 19, 683–696 (1980).

    Article  Google Scholar 

  14. Ward, C. W. & Dopheide, T. A. Biochem. J. (in the press).

  15. Springer, T. A. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. 73, 2481–2485 (1976).

    Article  CAS  ADS  Google Scholar 

  16. Coligan, J. E. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3390–3394 (1978).

    Article  CAS  ADS  Google Scholar 

  17. Tomita, M. & Marchesi, V. T. Proc. natn. Acad. Sci. U.S.A. 72, 2964–2968 (1975).

    Article  CAS  ADS  Google Scholar 

  18. Henderson, R. & Unwin, P. N. T. Nature 257, 28–32 (1975).

    Article  CAS  ADS  Google Scholar 

  19. Engelman, D. M., Henderson, R., McLachlan, A. D. & Wallace, B. A. Proc. Natn. Acad. Sci. U.S.A. 77, 2023–2027 (1980).

    Article  CAS  ADS  Google Scholar 

  20. Air, G. M. Virology 97, 468–472 (1979).

    Article  CAS  Google Scholar 

  21. McCauley, J. et al. FEBS. Lett 108, 422–426 (1979).

    Article  CAS  Google Scholar 

  22. Milstein, C., Brownlee, G. G., Harrison, T. M. & Mathews, M. B. Nature new Biol. 239, 117–120 (1972).

    Article  CAS  Google Scholar 

  23. Blobel, G. & Dobberstem, B. J. Cell Biol. 67, 835–851 (1975).

    Article  CAS  Google Scholar 

  24. Hirst, G. K. J. exp. Med. 75, 49–64 (1942).

    Article  CAS  Google Scholar 

  25. Lazarowitz, S. G. & Choppin, P. W. Virology 68, 440–454 (1975).

    Article  CAS  Google Scholar 

  26. Klenk. H. D., Rott, R., Orhch, M. & Blodorn, J. Virology 68, 426–439 (1975).

    Article  CAS  Google Scholar 

  27. Huang, R. T. C., Wahn, K., Klenk, H. D. & Rott, R. Virology 104, 294–302 (1980).

    Article  CAS  Google Scholar 

  28. Wiley, D. C., Wilson, I. A. & Skehel, J. J. Nature 289, 373–378 (1981).

    Article  CAS  ADS  Google Scholar 

  29. Wiley, D. C. & Skehel, J. J. J. molec. Biol. 112, 343–347 (1977).

    Article  CAS  Google Scholar 

  30. Monaco, H. L. thesis, Harvard Uinv. (1978).

  31. Harrison, S. C. J. appl. Crystallogr. l, 84–89 (1968).

    Article  Google Scholar 

  32. Wiley, D. C., Skehel, J. J. & Waterfield, M. D. Virology 79, 446–448 (1977).

    Article  CAS  Google Scholar 

  33. Bricogne, G. Acta crystallogr. A32, 832–847 (1976).

    Article  Google Scholar 

  34. Dopheide, T. A. & Ward, C. W. J. gen. Virol. (in the press).

  35. Waterfield, M. D., Scrace, G. & Skehel, J. J. Nature 289, 422–424 (1981).

    Article  CAS  ADS  Google Scholar 

  36. Harrison, S. C., Olson, A. J., Schutt, C. E., Winkler, F. K. & Bricogne, G. Nature 276, 368–373 (1978).

    Article  CAS  ADS  Google Scholar 

  37. Abad-Zapatero, C. et al. Nature 286, 33–39 (1980).

    Article  CAS  ADS  Google Scholar 

  38. Richardson, J. Adv. Protein Chem. (in the press).

  39. Wickner, W. A. Rev. Biochem. 48, 23–45 (1979).

    Article  CAS  Google Scholar 

  40. Air, G. M. in Structure and Variation in Influenza Virus (eds Laver, W. G. & Air, G. M.) 135–146 (Elsevier, Amsterdam, 1980).

    Google Scholar 

  41. Pauling, L. & Corey, R. B. Nature 171, 59–61 (1953).

    Article  CAS  ADS  Google Scholar 

  42. Cohen, C. & Holmes, K. C. J. molec. Biol. 6, 423–432 (1963).

    Article  CAS  Google Scholar 

  43. McLachlan, A. D. & Stewart, M. J. molec. Biol. 98, 293–304 (1975).

    Article  CAS  Google Scholar 

  44. Ward, C. W. & Dopheide, T. A. Aust. J. biol. Sci. 33, 449–455 (1980).

    Article  Google Scholar 

  45. Deisenhofer, J., Coleman, P. M., Epp, O. & Huber, R. Hoppe-Seyler's Z. physiol. Chem. 357, 1421–1434 (1976).

    Article  CAS  Google Scholar 

  46. Marquart, M., Deisenhofer, J., Huber, R. & Palm, W. in Proc. 7th Aharon Kelzir-Katchalsky Conf Ginossar (ed. Balasan, N.) (in the press).

  47. Laver, W. G., Air, G. M., Dopheide, T. A. & Ward, C. W. Nature 283, 454–457 (1980).

    Article  CAS  ADS  Google Scholar 

  48. Wright, C. S. J. molec. Biol. 141, 267–291 (1980).

    Article  CAS  Google Scholar 

  49. Gething, M. J., White, J. M. & Waterfield, M. D. Proc. natn. Acad. Sci. U.S.A. 75, 2737–2740 (1978).

    Article  CAS  ADS  Google Scholar 

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Author notes

  1. J. J. Skehel: National Institute for Medical Research, Mill Hill, London NW7 1AA, UK

Authors and Affiliations

  1. Gibbs Laboratory, Harvard University, 12 Oxford Street, Cambridge, Massachusetts, 02138

    I. A. Wilson, J. J. Skehel & D. C. Wiley

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  3. D. C. Wiley
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Wilson, I., Skehel, J. & Wiley, D. Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 Å resolution. Nature 289, 366–373 (1981). https://doi.org/10.1038/289366a0

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