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Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes

Nature volume 285, pages 367–373 (1980)Cite this article

Abstract

Specific initiation of transcription of cloned conalbumin and ovalbumin genes has been obtained in a cell-free system in the presence of purified calf thymus RNA polymerase B. Comparison with the adenovirus-2 early (E1 A) and major late genes reveals marked variations in transcriptional efficiencies. Specific fragmentation of the conalbumin gene DNA indicates that a short DNA segment 5′ to the initiation site is involved in specific transcription.

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Author notes

  1. O. Brison

    Present address: Department of Biochemistry, Stanford University, Stanford, California, 94305

Authors and Affiliations

  1. Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS et Unité 184 de Biologie Moléculaire et de Génie Génétique de I'NSERM, Faculté de Médecine, Strasbourg, France

    B. Wasylyk, C. Kédinger, J. Corden, O. Brison & P. Chambon

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  1. B. Wasylyk
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  2. C. Kédinger
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  3. J. Corden
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  4. O. Brison
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  5. P. Chambon
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Wasylyk, B., Kédinger, C., Corden, J. et al. Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes. Nature 285, 367–373 (1980). https://doi.org/10.1038/285367a0

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