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Embryonal carcinoma stem cells lack a function required for virus replication

Nature volume 285, pages 110–112 (1980)Cite this article

Abstract

Embryonal carcinoma (EC) stem cells are thought to be equivalent to the ectoderm of the preimplantation mouse embryo because they share morphological, biochemical and antigenic properties1,2. These near diploid EC cells can be induced to differentiate into cell types of all three germ layers1,2. They also can take part in normal differentiation and contribute cells to many tissues in chimaeric mice derived from preimplantation mouse embryos injected with them3. The ability to restrict the growth of certain viruses is a distinguishing characteristic of EC cells4–8, but it is intriguing that this restriction disappears after differentiation. The ability of EC cells to block totally productive infection by the highly infectious and oncogenic Moloney strain of murine leukaemia virus (M-MuLV) is remarkable, as virtually all other murine cells are permissive to the growth of this virus. Teich et al.7 suggested that replication of M-MuLV in EC cells is blocked at some point after integration of pro viral DNA into the cell's genome. Here I enquire into the dominant or recessive nature of the restriction, that is, do EC cells show transdominant inhibition of virus replication analogous to that imposed by interferon or the mouse Fv-l locus, or is the block to MuLV replication due to the lack of something in the EC cell? This report shows that the restriction of M-MuLV in EC cells can be overcome by fusion with permissive cells. The resulting complemented growth of M-MuLV in EC/permissive cell heterokaryons indicates that the restriction over M-MuLV is a recessive trait of EC cells and, therefore, defines a hitherto unknown host function that is vital to virus growth but missing in EC cells.

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  1. Department of Cellular and Developmental Immunology, Research Institute of Scripps Clinic, 10666 N. Torrey Pines Road, La Jolla, California, 92037

    James W. Gautsch

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  1. James W. Gautsch
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Gautsch, J. Embryonal carcinoma stem cells lack a function required for virus replication. Nature 285, 110–112 (1980). https://doi.org/10.1038/285110a0

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