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Complete nucleotide sequence of an influenza virus haemagglutinin gene from cloned DNA

Nature volume 282, pages 471–477 (1979)Cite this article

Abstract

A synthetic fowl plague virus (FPV) haemagglutinin gene has been cloned in bacteria and the complete sequence of the RNA gene deduced. It is 1,742 nucleotides long and the mRNA codes for 563 amino acids in an uninterrupted sequence. The nature of some of the important domains in the haemagglutinin has been established, and their structure is discussed in relation to their function. Extensive amino acid sequence homologies exist between FPV and human influenza haemagglutinins.

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References

  1. Inglis, S. C., Carroll, A. R., Lamb, R. A. & Mahy, B. W. J. Virology 74, 489–503 (1976).

    Article  CAS  Google Scholar 

  2. Scholtissek, C. Curr. Topics Microbiol. Immun. 80, 139–169 (1978).

    CAS  Google Scholar 

  3. McGeoch, D., Fellner, P. & Newton, C. Proc. natn. acad. Sci. U.S.A. 73, 3045–3049 (1976).

    Article  ADS  CAS  Google Scholar 

  4. Scholtissek, C., Harms, E., Rhode, W., Orlich, M. & Rott, R. Virology 74, 332–344 (1976).

    Article  CAS  Google Scholar 

  5. Inglis, S. C., McGeoch, D. & Mahy, B. W. J. Virology 78, 522–536 (1977).

    Article  CAS  Google Scholar 

  6. Almond, J. W. & Barry, R. D. Virology 92, 407–415 (1979).

    Article  CAS  Google Scholar 

  7. Hirst, G. K. J. exp. Med. 75, 49–64 (1942).

    Article  CAS  Google Scholar 

  8. Klenk, H. D., Rott, R., Orlich, M. & Blödorn, J. Virology 68, 426–439 (1975).

    Article  CAS  Google Scholar 

  9. Lazarowitz, S. G. & Choppin, P. W. Virology 68, 440–454 (1975).

    Article  CAS  Google Scholar 

  10. Drzeniek, R., Seto, J. T. & Rott, R. Biochim. biophys. Acta 128, 547–558 (1966).

    Article  Google Scholar 

  11. Laver, W. G. & Kilbourne, E. D. Virology 30, 493–501 (1966).

    Article  CAS  Google Scholar 

  12. Laver, W. G. & Webster, R. G. Br. med. Bull. 35, 29–33 (1979).

    Article  CAS  Google Scholar 

  13. Ward, C. W. & Dopheide, T. A. Br. med. Bull. 35, 51–56 (1979).

    Article  CAS  Google Scholar 

  14. Waterfield, M. D., Espelie, K., Elder, K. & Skehel, J. J. Br. med. Bull. 35, 57–63 (1979).

    Article  CAS  Google Scholar 

  15. Scholtissek, C., Rohde, W., Von Hoyningen, V. & Rott, R. Virology 87, 13–20 (1978).

    Article  CAS  Google Scholar 

  16. Palese, P. & Schulman, J. L. J. Virol. 17, 876–884 (1976).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Nakajima, K., Desselberger, U. & Palese, P. Nature 274, 334–339 (1978).

    Article  ADS  CAS  Google Scholar 

  18. Emtage, J. S., Catlin, G. H. & Carey, N. H. Nucleic Acids Res. 6, 1221–1240 (1979).

    Article  CAS  Google Scholar 

  19. Boyer, H. W. et al. in Recombinant Molecules : Impact on Science and Society (eds Beers, R. F. & Bassett, E. G.) 9–20 (Raven, New York, 1977).

    Google Scholar 

  20. Scheller, R. H., Dickerson, R. E., Boyer, H. W., Riggs, A. D. & Itakura, K. Science 196, 177–180 (1977).

    Article  ADS  CAS  Google Scholar 

  21. Hallewell, R. A. & Emtage, J. S. Gene (in the press).

  22. Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  23. Gillespie, D. & Spiegelman, S. J. molec. Biol. 12, 829–842 (1965).

    Article  CAS  Google Scholar 

  24. Vogelstein, B. & Gillespie, D. Proc. natn. Acad. Sci. U.S.A. 76, 615–619 (1979).

    Article  ADS  CAS  Google Scholar 

  25. Sanger, F. & Coulson, A. R. FEBS Lett. 87, 107–110 (1978).

    Article  CAS  Google Scholar 

  26. McReynolds, L. et al. Nature 273, 723–728 (1978).

    Article  ADS  CAS  Google Scholar 

  27. Roychoudhury, R., Jay, E. & Wu, R. Nucleic Acids Res. 3, 863–877 (1976).

    Article  CAS  Google Scholar 

  28. Skehel, J. J. & Hay, A. J. Nucleic Acids Res. 5, 1207–1219 (1978).

    Article  CAS  Google Scholar 

  29. Seeburg, P. H., Shine, J., Martial, J. A., Baxter, J. D. & Goodman, H. M. Nature 270, 486–494 (1977).

    Article  ADS  CAS  Google Scholar 

  30. Plotch, S. J., Bouloy, M. & Krug, R. M. Proc. natn. Acad. Sci. U.S.A. 76, 1618–1622 (1979).

    Article  ADS  CAS  Google Scholar 

  31. Skehel, J. J. & Hay, A. J. J. gen. Virol. 39, 1–8 (1978).

    Article  CAS  Google Scholar 

  32. Heindell, H. C., Liu, A., Paddock, G. V., Studnicka, G. M. & Salser, W. A. Cell 15, 43–54 (1978).

    Article  CAS  Google Scholar 

  33. Porter, A. G. et al. Nature 276, 298–301 (1978).

    Article  ADS  CAS  Google Scholar 

  34. McGeoch, D. J. & Turnbull, N. T. Nucleic Acids Res. 5, 4007–4024 (1978).

    Article  CAS  Google Scholar 

  35. Steitz, J. A. Nature 224, 957–964 (1969).

    Article  ADS  CAS  Google Scholar 

  36. Arrand, J. R. & Hindley, J. Nature new Biol. 244, 10–13 (1973).

    Article  CAS  Google Scholar 

  37. Squires, C. J. molec. Biol. 103, 351–381 (1976).

    Article  CAS  Google Scholar 

  38. Shine, J. & Dalgarno, L. Nature 254, 34–38 (1975).

    Article  ADS  CAS  Google Scholar 

  39. Steitz, J. A. J. molec. Biol. 73, 1–16 (1973).

    Article  CAS  Google Scholar 

  40. Emtage, J. S. et al. Nature (in the press).

  41. Post, L. E., Strycharz, G. D., Nomura, M., Lewis, H. & Dennis, P. P. Proc. natn. Acad. Sci. U.S.A. 76, 1697–1701 (1979).

    Article  ADS  CAS  Google Scholar 

  42. Nakanishi, S. Nature 278, 423–427 (1979).

    Article  ADS  CAS  Google Scholar 

  43. Subak-Sharpe, J. H. Br. med. Bull. 23, 161–168 (1967).

    Article  CAS  Google Scholar 

  44. Air, G. Virology 97, 468–472 (1979).

    Article  CAS  Google Scholar 

  45. Strauss, A. W., Bennett, C. D., Donohue, A. M., Rodkey, J. A. & Alberts, A. W. J. biol. Chem. 252, 6846–6855 (1977).

    CAS  PubMed  Google Scholar 

  46. Blobel, G. & Dobberstein, B. J. cell Biol. 67, 835–851 (1975).

    Article  CAS  Google Scholar 

  47. Rott, R., Klenk, H. D. & Scholtissek, C. in The Influenza Virus Hemagglutinin (eds Laver, W. G., Bachmayer, H. & Weil, R.) 69–81 (Springer, New York, 1978).

    Book  Google Scholar 

  48. Bosch, F. X., Orlich, M., Klenk, H. D. & Rott, R. Virology 95, 197–207 (1979).

    Article  CAS  Google Scholar 

  49. Klenk, H. D., Schwarz, R. T., Schmidt, M. F. G. & Wollert, W. in The Influenza Virus Hemagglutinin (eds Laver, W. G., Bachmayer, H. & Weil, R.) 83–99 (Springer, New York, 1978).

    Book  Google Scholar 

  50. Jackson, D. C., Dopheide, T. A., Russell, R. J., White, D. O. & Ward, C. W. Virology 93, 458–465 (1979).

    Article  CAS  Google Scholar 

  51. Gerhard, W. in The Influenza Virus Hemagglutinin (eds Laver, W. G., Bachmayer, H. & Weil, R.) 15–24 (Springer, New York, 1978).

    Book  Google Scholar 

  52. Porter, A. & Emtage, J. S. Biochem. Soc. Bull. 1, 72 (1979); Nucleotide Group Mtng, Cambridge (1979).

    Google Scholar 

  53. Robertson, J. S. Nucleic Acids Res. 6, 3745–3757 (1979).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Searle Research Laboratories, Lane End Road, High Wycombe, Bucks, UK

    A. G. Porter, C. Barber, N. H. Carey, R. A. Hallewell, G. Threlfall & J. S. Emtage

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  1. A. G. Porter
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  2. C. Barber
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  3. N. H. Carey
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  4. R. A. Hallewell
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  5. G. Threlfall
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  6. J. S. Emtage
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Porter, A., Barber, C., Carey, N. et al. Complete nucleotide sequence of an influenza virus haemagglutinin gene from cloned DNA. Nature 282, 471–477 (1979). https://doi.org/10.1038/282471a0

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