Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Haemolytic Efficiency of Rabbit IgG Anti-Forssman Antibody and its Augmentation by Anti-Rabbit IgG

Nature volume 216, pages 1295–1296 (1967)Cite this article

Abstract

Complement dependent lysis of an erythrocyte can be achieved in optimal conditions by reaction of a suitable anti-IgG with a single molecule of IgG antibody on the surface of an erythrocyte. Optimal conditions require special precautions to avoid antibodies which prevent C′ activation. For lysis of a sheep erythrocyte without augmentation, about 800 molecules of an IgG antibody were needed.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Borsos, T., and Rapp, H. J., J. Immunol., 95, 559 (1965).

    CAS  PubMed  Google Scholar 

  2. Humphrey, J. H., and Dourmashkin, R. R., in Ciba Foundation Symposiumon Complement (edit. by Wolstenholme, G. E. W., and Knight, J.), 175 (Churchill, London, 1965).

    Book  Google Scholar 

  3. Borsos, T., and Rapp, H. J., Science, 150, 505 (1965).

    Article  ADS  CAS  Google Scholar 

  4. Humphrey, J. H., and Dourmashkin, R. R. (in the press).

  5. Jerne, N. K., Nordin, A. A., and Henry, C., in Cell Bound Antibodies (edit. by Amos, B., and Koprowski, H.), 109 (Wistar Institute Press, 1963).

    Google Scholar 

  6. Ingraham, J. S., and Bussard, A., J. Exper. Med., 119, 667 (1964).

    Article  CAS  Google Scholar 

  7. S̆terzl, J., and R̆íha, I., Nature, 208, 858 (1965).

    Article  ADS  Google Scholar 

  8. Dresser, D. W., and Wortis, H., Nature, 208, 859 (1965).

    Article  ADS  CAS  Google Scholar 

  9. Mayer, M. M., Exper. Immunochem. (edit. by Kabat, E. A., and Mayer, M. M.), 149 (Thomas, Springfield, Illinois, 1961).

    Google Scholar 

  10. Benacerraf, B., Ovary, Z., Bloch, K. J., and Franklin, E. C., J. Exper. Med., 117, 937 (1963).

    Article  CAS  Google Scholar 

  11. Oettgen, H. F., Binaghi, R. A., and Benacerraf, B., Proc. Soc. Exper. Biol. NY, 118, 336(1965).

    Article  CAS  Google Scholar 

  12. Nakamura, H., and Katsura, T., Japan J. Exper. Med., 34, 167 (1964).

    CAS  Google Scholar 

  13. Lavergne, M., Raynaud, M., and Iscaki, S., Ann. Inst. Pasteur, 110, supp. 3, 155 (1966).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute for Medical Research, London, NW7

    J. H. HUMPHREY

Authors
  1. J. H. HUMPHREY
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

HUMPHREY, J. Haemolytic Efficiency of Rabbit IgG Anti-Forssman Antibody and its Augmentation by Anti-Rabbit IgG. Nature 216, 1295–1296 (1967). https://doi.org/10.1038/2161295a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/2161295a0

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing