Abstract
AT present thick (∼ 50µ) frozen sections of fixed tissues are commonly utilized for cytochemistry and electron microscopy1–3 despite the fact that freezing and thawing are known to cause disruption of fine structure and loss of enzymatic activity even in fixed tissues3,4. These disadvantages have been tolerated since use of sections not exceeding 50µ is necessary to ensure even penetration of incubation media1,2, and no alternative sectioning method has been available.
Similar content being viewed by others
Article PDF
References
Novikoff, A. B., Essner, E., Goldfischer, S., and Heus, M., in The Interpretation of Ultrastructure (Academic Press, New York and London, 1962).
Holt, S. J., and Hicks, R. M., J. Biophys. Biochem. Cytol., 11, 31 (1961).
Sabatini, D. D., Bensch, K., and Barrnett, R. F., J. Cell Biol., 17, 19 (1963).
Novikoff, A. B., Burnett, F., and Glickman, M., J. Histochem. Cytochem., 4, 416 (1956).
McIlwain, H., and Buddin, H. L., Biochem. J., 53, 412 (1953).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SMITH, R., FARQUHAR, M. Preparation of Thick Sections for Cytochemistry and Electron Microscopy by a Non-freezing Technique. Nature 200, 691 (1963). https://doi.org/10.1038/200691a0
Issue Date:
DOI: https://doi.org/10.1038/200691a0
This article is cited by
-
Cytochemical localization of β-NADPase and TMPase in B cells of pancreas
International journal of pancreatology (1989)
-
Polyethylene glycol embedded tissue sections for immunoelectronmicroscopy
Histochemistry (1981)
-
Quantitative estimate of pinocytosis in experimental acute hypertension
Acta Neuropathologica (1979)
-
A cytochemical and ultrastructural study of the ?S.I.F.? cells in cat sympathetic ganglia
Histochemistry (1979)
-
Acetylcholinesterase activity in the normal and retino-deprived optic tectum of the quail
Histochemistry (1978)
