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Translocation of a calcium-permeable cation channel induced by insulin-like growth factor-I

Nature Cell Biology volume 1, pages 165–170 (1999)Cite this article

Abstract

Calcium plays a critical part in the regulation of cell growth, and growth factors stimulate calcium entry into cells through calcium-permeable channels. However, the molecular nature and regulation of calcium-permeable channels are still unclear at present. Here we report the molecular characterization of a calcium-permeable cation channel that is regulated by insulin-like growth factor-I (IGF-I). This channel, which we name growth-factor-regulated channel (GRC), belongs to the TRP-channel family and localizes mainly to intracellular pools under basal conditions. Upon stimulation of cells by IGF-I, GRC translocates to the plasma membrane. Thus, IGF-I augments calcium entry through GRC by regulating trafficking of the channel.

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Figure 1: Structural characteristics and tissue expression patterns of GRC.
Figure 2: Characterization of GRC as an IGF-I-regulated, calcium-permeable, non-selective cation channel.
Figure 3: Translocation of GRC from intracellular compartments to the plasma membrane.
Figure 4: Translocation of endogenous GRC in MIN6 cells.

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Acknowledgements

This study was supported by Grants-in-Aid for Scientific Research on Priority Area from the Ministry of Education, Science, Sports and Culture of Japan. We thank J. Takeda and K. Takata for discussions.

Correspondence and requests for materials should be addressed to I.K. The cDNA sequence for mouse GRC has been deposited at GenBank under accession number AB021665.

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  1. Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, 371-8512, Japan

    Makoto Kanzaki, You-Qing Zhang*, Hirosato Mashima*, Lu Li*, Hiroshi Shibata* & Itaru Kojima

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  1. Makoto Kanzaki
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Correspondence to Itaru Kojima.

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Kanzaki, M., Zhang*, YQ., Mashima*, H. et al. Translocation of a calcium-permeable cation channel induced by insulin-like growth factor-I. Nat Cell Biol 1, 165–170 (1999). https://doi.org/10.1038/11086

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