Abstract
The neuropeptides substance P (SP), calcitonin gene-related peptide (CGRP) and α-melanocyte-stimulating hormone (α-MSH) are known to be able to regulate the production of cytokines in the skin. Since IL-8 plays an important role in cutaneous inflammation, the effects of SP, CGRP and α-MSH on the IL-8/IL-8 receptor (IL-8RA) systems of these cell types were studied. Cultures of human dermal fibroblasts and an immortalized keratinocyte cell line HaCaT were treated with 10−8 M SP, CGRP or α-MSH. The results demonstrated that these neuropeptides have different effects on the IL-8 and IL-8RA expressions of the cells. SP and CGRP upregulated the IL-8RA mRNA expression in HaCaT cells, but had no influence on their IL-8 production, whereas, α-MSH had no effect on either the IL-8 or the IL-8RA mRNA expression in HaCaT cells. In contrast, α-MSH resulted in a time-dependent induction of the IL-8 mRNA expression in dermal fibroblasts. This induction was already detectable after 6 h, and after 12 h there was a 5-fold change in comparison with the controls. The IL-8 content of the supernatant was also increased, with a maximum at 48 h after α-MSH treatment. The data established in the present study support the notion that neuropeptides can directly modulate the IL-8/IL-8RA system of keratinocytes and fibroblasts.
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Kiss, M., Kemény, L., Gyulai, R. et al. Effects of the Neuropeptides Substance P, Calcitonin Gene-Related Peptide and α-Melanocyte-Stimulating Hormone on the IL-8/IL-8 Receptor System in a Cultured Human Keratinocyte Cell Line and Dermal Fibroblasts. Inflammation 23, 557–567 (1999). https://doi.org/10.1023/A:1020294507767
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DOI: https://doi.org/10.1023/A:1020294507767