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Characterization of non-solvent- and thermal-induced phase separation applied polycaprolactone/demineralized bone matrix scaffold for bone tissue engineering

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Abstract

Objective

Polycaprolactone (PCL) is a widely applied biomaterial in bone tissue engineering (BTE) due to its superior mechanical properties and biodegradability. However, the high hydrophobicity and low cell adhesion properties of PCL show limited cell interactions. Herein, we prepared the porous PCL/DBP composites with improved cell adhesion through the addition of demineralized bone powder (DBP). Three-dimensional scaffolds were fabricated by mixing various concentrations of DBP with PCL and applying non-solvent-induced phase separation (NIPS) and thermal-induced phase separation (TIPS) (N-TIPS) and solvent casting and particulate leaching (SCPL) to impart porosity.

Methods

A characteristic evaluation was performed through X-ray diffraction (XRD), morphological analysis, physicochemical analysis, bioactivity test, and mechanical test. Upon culture with mouse bone marrow stem cells (mBMSCs), proliferation and osteogenic differentiation of mBMSC were evaluated using quantitative dsDNA analysis and alkaline phosphatase (ALP) activity, respectively.

Results

The addition of DBP improved the physicochemical and mechanical properties of the scaffold and formed a large amount of hydroxyapatite (HAp). Also, cell proliferation and differentiation were increased by enhancing cell adhesion.

Conclusion

The porous PCL/DBP scaffolds could provide a favorable microenvironment for cell adhesion and be a promising biomaterial model for bone tissue engineering.

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Funding

This research was supported by the bilateral cooperation Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2019K2A9A1A06098563).

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Authors and Affiliations

  1. Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk, 54896, Korea

    Soo in Kim, Na Eun Kim, Sunjae Park, Joo Hee Choi, Younghun Lee, Gayeong Jeon, Jeong Eun Song & Gilson Khang

  2. Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk, 54896, Korea

    Gilson Khang

  3. Department of Orthopaedic & Traumatology, Airlangga University, Jl. Airlangga No.4-6, Airlangga, Kec. Gubeng, Kota SBY, Jawa Timur, 60115, Indonesia

    Gilson Khang

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  1. Soo in Kim
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Contributions

S.I.K. and N.E.K. are equally contributed as first author. S.I.K., N.E.K., S.J.P., and J.H.C. designed and conceived the study. S.J.P. helped write the manuscript. Y.G.L. and G.Y.J. fabricated and characterized the material. S.I.K. and N.E.K. performed the in vitro study. J.E.S. and G.K. gave feedback and opinion on the study. G.K. contributed in reagents and materials and supervised overall study.

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Correspondence to Gilson Khang.

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Kim, S.i., Kim, N.E., Park, S. et al. Characterization of non-solvent- and thermal-induced phase separation applied polycaprolactone/demineralized bone matrix scaffold for bone tissue engineering. In vitro models 1, 197–207 (2022). https://doi.org/10.1007/s44164-022-00018-9

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