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A Panel of Plasma miRNAs 199b-3p, 224-5p and Let-7d-3p as Non-Invasive Diagnostic Biomarkers for Endometriosis

  • Endometriosis: Original Article
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A Correction to this article was published on 01 February 2021

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Abstract

The objective of this study was to investigate whether the combination of miR-224-5p, miR-199-3p, and let-7d-3p is a suitable diagnostic panel for endometriosis. Twenty-five women with endometriosis (case) and twenty-five women without any sign of endometriosis (controls) were included. Peripheral blood specimens were collected from all these women who were a proper candidate for laparoscopy before surgery. Total RNA was isolated to synthesize complementary DNA. Expression of miR-199b-3p, miR-224-5p, and let-7d-3p was analyzed by RT-qPCR. To estimate the performance of the identified miRNAs for endometriosis diagnosis, we performed ROC curves analysis. There was an upregulation of miRNAs 199b-3p (P value < 0.001) and down-regulation of 224-5p (P value < 0.001) and miRNA let-7d-3p (P value < 0.05) in women with endometriosis compared to non-endometriosis women. The diagnostic accuracy of miRNAs 199b-3p, 224-5p, and let-7d-3p was measured by AUC which was 0.843 (sensitivity = 96% and specificity = 80%), 0.914 (sensitivity = 84% and specificity = 80%), and 0.696 (sensitivity = 80% and specificity = 56%) for miRNAs 199b-3p, 224-5p, and let-7d-3p, respectively. In combination, they showed the highest accuracy with the AUC 0.992 (sensitivity = 96% and specificity = 100%). In conclusion(s) the levels of miRNAs 199b-3p, 224-5p, and Let-7d-3p in plasma are potential diagnostic biomarkers for endometriosis patients.

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All data and material of the current study are accessible and comply with field standards.

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Acknowledgments

This study is part of the MSc thesis of the first author and was supported by Tehran University of Medical Sciences (Grant No: 36424) and is acknowledged by authors.

Funding

This study was financially supported by the Research Department of Tehran University of Medical Sciences (TUMS) (Grant No: 36424).

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Authors and Affiliations

  1. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Narges Zafari & Pantea Izadi

  2. Department of Gynecology and Obstetrics, Tehran University of Medical Sciences, Tehran, Iran

    Azam Manshadi Tarafdari

  3. Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Mehrdad Noruzinia & Afshin Bahramy

  4. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Mir Saead Yekaninejad

  5. Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Ali Mohebalian

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  1. Narges Zafari
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  3. Pantea Izadi
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  4. Mehrdad Noruzinia
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Correspondence to Pantea Izadi.

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The authors declare that that they have no conflict of interest in this study.

Ethics Approval

The questionnaire and methodology for this study was approved by the Human Research Ethics committee of the Tehran University of Medical science (Ethics approval number: IR.TUMS.MEDICINE.REC.1396.3898).

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This article was updated to correct an error in the title and in the sixth sentence of the abstract.

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Zafari, N., Tarafdari, A.M., Izadi, P. et al. A Panel of Plasma miRNAs 199b-3p, 224-5p and Let-7d-3p as Non-Invasive Diagnostic Biomarkers for Endometriosis. Reprod. Sci. 28, 991–999 (2021). https://doi.org/10.1007/s43032-020-00415-z

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  • DOI: https://doi.org/10.1007/s43032-020-00415-z

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