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Genome-wide identification of glutathione peroxidase (GPX) gene family and their response to abiotic stress in cucumber

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Abstract

Plant glutathione peroxidases (GPXs) are non-heme thiol peroxidases that play vital roles in maintaining H2O2 homeostasis and regulating plant response to abiotic stress. Here, we performed a comparative genomic analysis of the GPX gene family in cucumber (Cucumis sativus). As a result, a total of 6 CsGPX genes were identified, which were unevenly located in four out of the seven chromosomes in cucumber genome. Based on the phylogenetic analysis, the GPX genes of cucumber, Arabidopsis and rice could be classified into five groups. Analysis of the distribution of conserved domains of GPX proteins showed that all these proteins contain three highly conserved motifs, as well as other conserved sequences and residues. Gene structure analysis revealed a conserved exon–intron organization pattern of these genes. Through analyzing the promoter regions of CsGPX genes, many hormone-, stress-, and development-responsive cis-elements were identified. Moreover, we also investigated their expression patterns in different tissues and developmental stages as well as in response to abiotic stress and x acid (ABA) treatments. The qRT-PCR results showed that the transcripts of CsGPX genes varied largely under abiotic stress and ABA treatments at different time points. These results demonstrate that cucumber GPX gene family may function in tissue development and plant stress responses.

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Acknowledgements

This work was funded by the Key Project of Youth Science Foundation of Jiangxi Province (20171ACB21025), and the National Natural Science Foundation of China (31460522 and 31660578).

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  1. Yong Zhou and Lifang Hu contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, College of Science, Jiangxi Agricultural University, Nanchang Economic and Technological Development District, Nanchang, 330045, Jiangxi, China

    Yong Zhou, Lunwei Jiang & Shiqiang Liu

  2. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, College of Agronomy, Jiangxi Agricultural University, Nanchang Economic and Technological Development District, Nanchang, 330045, Jiangxi, China

    Lifang Hu

  3. Shanghai Agrobiological Gene Center, Shanghai, 201106, China

    Shuifeng Ye

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  1. Yong Zhou
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Correspondence to Shiqiang Liu.

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Zhou, Y., Hu, L., Ye, S. et al. Genome-wide identification of glutathione peroxidase (GPX) gene family and their response to abiotic stress in cucumber. 3 Biotech 8, 159 (2018). https://doi.org/10.1007/s13205-018-1185-3

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