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Kefir: A Multifaceted Fermented Dairy Product

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Abstract

Kefir is a fermented dairy beverage produced by the actions of the microflora encased in the “kefir grain” on the carbohydrates in the milk. Containing many bacterial species already known for their probiotic properties, it has long been popular in Eastern Europe for its purported health benefits, where it is routinely administered to patients in hospitals and recommended for infants and the infirm. It is beginning to gain a foothold in the USA as a healthy probiotic beverage, mostly as an artisanal beverage, home fermented from shared grains, but also recently as a commercial product commanding shelf space in retail establishments. This is similar to the status of yogurts in the 1970s when yogurt was the new healthy product. Scientific studies into these reported benefits are being conducted into these health benefits, many with promising results, though not all of the studies have been conclusive. Our review provides an overview of kefir’s structure, microbial profile, production, and probiotic properties. Our review also discusses alternative uses of kefir, kefir grains, and kefiran (the soluble polysaccharide produced by the organisms in kefir grains). Their utility in wound therapy, food additives, leavening agents, and other non-beverage uses is being studied with promising results.

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References

  1. James F (1975) Yogurt: its life and culture. Exped Fall 11(1):32–38

  2. Kabak B, Dobson A (2011) An introduction to the traditional fermented foods and beverages of Turkey. Crit Rev Food Sci 51(3):248–260

    Google Scholar 

  3. Ötles S, Çagindi Ö (2003) Kefir: a probiotic dairy-composition, nutritional and therapeutic aspects. Pak J Nutr 2(2):54–59

    Google Scholar 

  4. Farnworth ER (2006) Kefir: a complex probiotic. Food Sci Technol B 2:1–17

    Google Scholar 

  5. Marshall VM, Cole WM, Brooker B (1984) Observations on the structure of kefir grains and the distribution of the microflora. J Appl Bacteriol 57:491–497

    Google Scholar 

  6. Yamin H, Elmali M, Karadagoglu G, Cetinkaya A (2006) Observations of kefir grains and their structure from different geographical regions: Turkey and Germany. Ataturk Universitesi Vet Bil Derg 1(1–2):11–15

    Google Scholar 

  7. Garrote GL, Abraham AG, de Antoni GL (2001) Chemical and microbiological characteristization of kefir grains. J Dairy Res 68:639–652

    CAS  Google Scholar 

  8. Simova E, Beshkova D, Angelov A, Hristozova T, Frengova G, Spasov Z (2002) Lactic acid bacteria and yeasts in kefir grains and kefir made from them. J Ind Microbiol Biotechnol 28:1–6

    CAS  Google Scholar 

  9. Beshkova DM, Simova E, Simov Z, Frengova G, Spasov Z (2002) Pure cultures for making kefir. Food Microbiol 19:537–544

    Google Scholar 

  10. Witthuhn R, Schoeman T, Britz T (2005) Characterization of the microbiological population at different stages of kefir production and kefir grain mass cultivation. Int Dairy J 15:383–389

    CAS  Google Scholar 

  11. Dobson A, O’Sullivan O, Cotter PD, Ross P, Hill C (2011) High- throughput sequence-based analysis of the bacterial composition of kefir and an associated kefir grain. FEMS Microbiol Lett 320:56–62

    CAS  Google Scholar 

  12. Ertekin B, Guzel-Seydim ZB (2010) Effect of fat replacers on kefir quality. J Sci Food Agric 90:543–548

    CAS  Google Scholar 

  13. Cui X, Chen S, Wang Y, Han J (2013) Fermentation conditions of walnut milk beverage inoculated with kefir grains. LWT Food Sci Technol 50:349–352

    CAS  Google Scholar 

  14. Puerani C, Magalhães KT, Schwan RF (2012) New cocoa pulp-based kefir beverages: microbiological, chemical composition and sensory analysis. Food Res Int 48:634–640

    Google Scholar 

  15. Liu J, Lin C (2000) Production of kefir from soymilk with or without added glucose, lactose, or sucrose. J Food Microbiol Saf 65(4):716–719

    CAS  Google Scholar 

  16. Liu J, Chen M, Lin C (2005) Antimutagenic and antioxidant properties of milk-kefir and soymilk-kefir. J Agric Food Chem 53:2467–2474

    CAS  Google Scholar 

  17. Bensmira M, Jiang B (2011) Organic acids formation during the production of a novel peanut-milk kefir beverage. Br J Dairy Sci 2(1):18–22

    CAS  Google Scholar 

  18. Farnworth ER (1999) Kefir: from folklore to regulatory approval. J Nutraceut Funct Med Foods 1(4):57–68

    Google Scholar 

  19. Margulis L (1995) From kefir to death. In: Brockman J, Matson K (eds) How things are: a science tool-kit for the mind. William Morrow, New York, pp 69–78

  20. Trenev N (1998) Probiotics: nature’s internal healers. Avery, Garden City Park, p 11

    Google Scholar 

  21. Pintado ME, DaSilva JL, Fernandes PB, Malcata CX, Hogg TA (1996) Microbiological and rheological studies on Portuguese kefir grains. Int J Food Sci Technol 31:15–36

    CAS  Google Scholar 

  22. Santos A, San Mauro M, Sanchez A, Torres J, Marquina D (2003) The antimicrobial properties of different strains of Lactobacillus spp. isolated from kefir. Syst Appl Microbiol 26:434–437

    CAS  Google Scholar 

  23. Rea M, Lennartsson T, Dillon P, Drinan F, Reville W, Heapes M, Cogan T (1996) Irish kefir-like grains: their structure, microbial composition and fermentation kinetics. J Appl Bacteriol 81:83–94

    Google Scholar 

  24. Thompson J, Johnson D, Murphy R, Collins M (1990) Characteristics of a milk fermentation from rural northern Ireland which resembles kefir. Ir Food Sci Technol 14:35–49

    Google Scholar 

  25. Irwin F (1949) The cookin’ woman. Oliver and Boyd, London, pp 210–211

    Google Scholar 

  26. Farnworth ER, Mainville I (2003) Kefir: a fermented milk product. In: Farnworth ER (ed) Handbook of fermented functional foods. CRC Press, Boca Raton, pp 78–111

  27. Kolakowski P, Ozimkiewicz M (2012) Restoration of kefir grains subjected to different treatments. Int J Dairy Technol 65(1):140–145

    CAS  Google Scholar 

  28. Angulo L, Lopez E, Lema C (1993) Microflora present in kefir grains of the Galician region (north-west of Spain). J Dairy Res 60:263–267

    CAS  Google Scholar 

  29. Irigoyen A, Arana I, Castiella M, Torre P, Ibáñez F (2005) Microbiological, physiochemical, and sensory characteristics of kefir during storage. Food Chem 90:613–620

    CAS  Google Scholar 

  30. Urdaneta E, Barrenetxe J, Aranguren P, Irigoyen A, Marzo F, Ibáñez FC (2007) Intestinal beneficial effects of Kefir-supplemented diet in rats. Nutr Res 27:653–658

    CAS  Google Scholar 

  31. Guzel-Seydim Z, Wyffels JT, Seydim AC, Greene AK (2005) Turkish kefir and kefir grains: microbial enumeration and electron microscobic observation. Int J Dairy Technol 58:25–29

    Google Scholar 

  32. Motaghi M, Moazami M, Farkhondeh A, Fooladi M, Goltapeh E (1997) Short Communication: kefir production in Iran. World J Microb Biotechnol 13:578–581

    Google Scholar 

  33. Ghasemlou M, Khodaiyan F, Oromiehie A, Yarmand M (2011) Development and characterization of a new biodegradable edible film made from kefiran, an exopolysaccharide obtained from kefir grains. Food Chem 127:1495–1502

    Google Scholar 

  34. Xie N, Zhou T, Li B (2012) Kefir yeasts enhance probiotic potentials of Lactobacillus paracasei H9: the positive effects of coaggregation between the two strains. Food Res Int 45:394–401

    CAS  Google Scholar 

  35. Huang Y, Wu F, Wang X, Sul Y, Yang L, Wang J (2013) Characterization of Lactobacillus plantarum Lp27 isolated from Tibetan kefir grains: a potential probiotic bacterium with cholesterol-lowering effects. J Dairy Sci 96:2816–2825

    CAS  Google Scholar 

  36. Wang Y, Xu N, Xi A, Ahmed Z, Zhang B, Bai X (2009) Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microflora of rats fed on high-cholesterol diets. Appl Microbiol Biotechnol 84:341–347

    CAS  Google Scholar 

  37. Shiomi M, Sasaki K, Murofushi M, Aibara K (1982) Antitumor activity in mice of orally administered polysaccharide from kefir grain. Jpn J Med Sci Biol 35:75–80

    CAS  Google Scholar 

  38. Chen T, Wang S, Chen K, Liu J, Chen M (2009) Microbiological and chemical properties of kefir manufactured by entrapped microorganisms isolated from kefir grains. J Dairy Sci 92:3002–3013

    CAS  Google Scholar 

  39. Liu JR, Wang SY, Chen MJ, Yeuh PY, Lin CW (2006) The anti-allergenic properties of milk and soymilk kefir and their beneficial effects on the intestinal microflora. J Sci Food Agric 86:2527–2533

    CAS  Google Scholar 

  40. Leite A, Leite D, DelAguila E, Alvares T, Peixoto R, Miguel M, Silva J (2013) Microbiological and chemical characterization of Brazilian kefir during fermentation and storage processes. J Dairy Sci 96:4149–4159

    CAS  Google Scholar 

  41. Silva KR, Rodrigues SA, Filhom LX, Lima AS (2009) Antimicrobial activity of broth fermented with kefir grains. Appl Biochem Biotechnol 152:316–325

    CAS  Google Scholar 

  42. Rimada PS, Abraham AG (2001) Polysaccharide production by kefir grains during whey fermentation. J Dairy Res 68:653–661

    CAS  Google Scholar 

  43. Witthuhn RC, Cilliers A, Britz TJ (2005) Evaluation of different preservation techniques on the storage potential of kefir grains. J Dairy Res 72:125–128

    CAS  Google Scholar 

  44. Powell JE, Witthuhn RC, Todorov SD, Dicks LMT (2007) Characterization of bacteriocin ST8KF produced by a kefir isolate Lactobacillus plantarum ST8KF. Int Dairy J 17:190–198

    CAS  Google Scholar 

  45. Mayo B, Ammor MS, Delgado S, Alegria Á (2010) Fermented milk products. In: Tamang JP, Kailasapathy K (eds) Fermented foods and beverages of the world. CRC Press, Boca Raton, pp 278–280

  46. Marsh AJ, O’Sullivan O, Hill C, Ross RP, Cotter PD (2013) Sequencing-based analysis of the bacterial and fungal composition of kefir grains and milks from multiple sources. PLoS One 8(7):e69171. doi:10.1371/journal.pone0069371

    Google Scholar 

  47. Margulis L (1994) Sex, death and kefir. Sci Am 271(2):96

  48. Bottazzi V, Bianchi F (1980) A note on scanning electron microscopy of micro-organisms associated with the kefir granule. J Appl Bacteriol 48:265–268

    Google Scholar 

  49. Magalhães KT, Pereira GVDM, Campos CR, Dragone G, Schwan RF (2011) Brazilian Kefir: structure, microbial communities and chemical composition. Braz J Microbiol 42:693–702

    Google Scholar 

  50. Leite AMO, Miguel MOL, Peixiti RS, Rosado AS, Silva JT, Paschoalin VMF (2013) Microbiological, technological and therapeutic properties of kefir: a natural probiotic beverage. Braz J Microbiol 44(2):341–344

    Google Scholar 

  51. Wang SY, Chen KN, Lo YM, Chiang ML, Chen HC, Liu JR, Chen MJ (2012) Investigation of microorganisms involved in biosynthesis of the kefir grain. Food Microbiol 32:274–285

    Google Scholar 

  52. Tamine A, Muir D, Wszolek M (1999) Kefir, koumiss, and kishk. Dairy Ind Int 64(5):32–33

    Google Scholar 

  53. Bolla PA, de los Serradell A, de Urraza PJ, De Antoni GL (2011) Effect of freeze-drying on viability and in vitro probiotic properties of a mixture of lactic acid bacteria and yeasts isolated from kefir. J Dairy Res 78:15–22

    CAS  Google Scholar 

  54. Wszolek M, Kupiec-Teahan B, Guldager HS, Tamine A (2006) Production of kefir, koumiss, and other related products. In: Tamime AY (ed) Fermented milks. Blackwell Science, Oxford, pp 174–216

  55. Chen HC, Lin CW, Chen MJ (2006) The effects of freeze drying and rehydration on survival of microorganisms in kefir. Asian Aust J Anim Sci 19(1):126–130

    Google Scholar 

  56. Muyzer G, DeWaall E, Uitterlinden A (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700

    CAS  Google Scholar 

  57. Fasoli S, Marzotto M, Rizzotti L, Dellaglio F, Torriani S (2003) Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysis. Int J Food Microbiol 82:59–70

    CAS  Google Scholar 

  58. Masoud W, Cesar L, Jespersen L, Jakobsen M (2004) Yeast involved in fermentation of Coffea arabica in East Africa determined by genotyping and by direct denaturating gradient gel electrophoresis. Yeast 21:549–556

    CAS  Google Scholar 

  59. Theunissen J, Britz T, Torriani S, Witthuhn R (2005) Identification of probiotic microorganisms in South African products using PCR-based DGGE analysis. Int J Food Microbiol 98:11–21

    CAS  Google Scholar 

  60. Leite A, Mayo B, Rachid C, Peixoto RS, Silva JT, Paschoalin V, Delgado S (2012) Assessment of the microbial diversity of Brazilian kefir grains by PCR-DGGE and pyrosequencing analysis. Food Microbiol 31:215–221

    CAS  Google Scholar 

  61. Chagnaud P, Machinis K, Coutte L, Marecat A, Mercenier A (2001) Rapid PCR-based procedure to identify lactic acid bacteria: application to six common Lactobacillus species. J Microbiol Methods 44:139–148

    CAS  Google Scholar 

  62. Kullen M, Sanozky-Dawes R, Crowell D, Klaenhammer T (2000) Use of the DNA sequence of variable regions of the 16S rRNA. J Appl Microbiol 89:511–516

    CAS  Google Scholar 

  63. Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM, Munro K, Alatossava T (2000) Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Appl Environ Microbiol 66:297–303

    CAS  Google Scholar 

  64. Delfederico L, Hollmann A, Martinez M, Iglesias NG, DeAntoni G, Semorile L (2006) Molecular identification and typing of lactobacilli isolated from kefir grains. J Dairy Res 73:20–27

    CAS  Google Scholar 

  65. Golowczyc MA, Gugliada MJ, Hollmann A, Delfederico L, Garrote GL, Abraham AG, Semorile L, De Antoni G (2008) Characterization of homofermentative lactobacilli isolated from kefir grains: potential use as probiotic. J Dairy Res 75:211–217

    CAS  Google Scholar 

  66. Dobson A, O’Sullivan O, Cotter P, Ross P, Hill C (2011) High-throughput sequence-based analysis of the bacterial composition of kefir and an associated kefir grain. FEMS Microbiol Letters 320:56–62

    CAS  Google Scholar 

  67. Kök Tas T, Ekinci F, Guzel-Seydim Z (2011) Identification of microbial flora in kefir grains produced in Turkey using PCR. Int J Dairy Technol 65:126–131

    Google Scholar 

  68. Singh S, Goswami P, Singh R, Heller K (2009) Application of molecular identification tool for Lactobacillus, with a focus on discrimination between closely related species: a review. LWT Food Sci Technol 42:448–457

    CAS  Google Scholar 

  69. Song Y, Kato N, Liu C, Matsumiya Y, Kato H, Watanabe K (2000) Rapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group and species-specific primers derived from the 16S–23S rRNA intergenic spacer region and its flanking 23S rRNA. FEMS Microbiol Lett 187:167–173

    CAS  Google Scholar 

  70. Wang S, Chen H, Liu J, Lin Y, Chen M (2008) Identification of yeasts and evaluation of their distribution in Taiwanese kefir and viili starters. J Dairy Sci 91:3798–3805

    CAS  Google Scholar 

  71. Magalhães K, Pereira M, Nicolau A, Dragone G, Domingues L, Teixeira J, Silva J, Schwan R (2010) Production of fermented cheese whey-based beverage using kefir grains as starter culture: evaluation of morphological and microbial variations. Bioresour Technol 101:8843–8850

    Google Scholar 

  72. Marshall V (1987) Fermented milks and their future trends I. Microbial aspects. J Dairy Res 54:559–574

    CAS  Google Scholar 

  73. Nambou K, Gao C, Zhou F, Guo B, Ai L, Wu ZJ (2014) A novel approach of direct formulation of defined starter cultures for different kefir-like beverage production. Int Dairy J 34(2):237–246

    CAS  Google Scholar 

  74. Sarkar S (2008) Biotechnological innovations in kefir production: a review. Br Food J 110(3):283–295

    Google Scholar 

  75. Guzel-Sedam ZB, Kok-Tas T, Greene AK, Seydim AC (2011) Review: Functional properties of kefir. Crit Rev Food Sci 51(3):261–268

    Google Scholar 

  76. Ahmed Z, Wang Y, Ahmad A, Khan ST, Nisa M, Ahmad H, Afreen A (2013) Kefir and health: a contemporary perspective. Crit Rev Food Sci 53(5):422–434

    Google Scholar 

  77. Fuller R (1992) Probiotics: the scientific basis. Chapman and Hall, London, p 1

    Google Scholar 

  78. FAO/WHO (2001) Report of a joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Available at: http://www.who.int/foodsafety/publications/fs_management/en/probiotics.pdf. (Accessed 14 July 2014)

  79. Salminen S, Isolauri E, Salminen E (1996) Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges. Antonie von Leewenhoek 70:347–358

    CAS  Google Scholar 

  80. Ötles S, Çagindi Ö, Akçiçek ES, Cagindi O, Akcicek E (2003) Probiotics and health. Asian Pac J Cancer Prev 4:369–372

    Google Scholar 

  81. Metchnikoff É (1908) The prolongation of life: optimistic studies. In: Mitchell PC (ed) Arno press, literature of death and dying, 1977th edn. The Knickerbocker Press, New York

    Google Scholar 

  82. Sezer G, Güven A (2009) Investigation of bacteriocin production capability of lactic acid bacteria isolated from foods. Kafkas Univ Vet Fak Derg 15(1):45–50

    Google Scholar 

  83. Bolla PA, Carasi P, de los Serradell A, De Antoni GL (2013) Kefir-isolated Lactococcus lactis subsp. lactis inhibits the cytotoxic effects of Clostridium difficile in vitro. J Dairy Res 80:96–102

    CAS  Google Scholar 

  84. Kakisu E, Abraham AG, Farinati CT, Ibarra C (2013) Lactobacillus plantarum isolated from kefir protects vero cells from cytotoxicity by type II shiga toxin from Escherichia coli O157:H7. J Dairy Res 80:64–71

    CAS  Google Scholar 

  85. Kakisu E, Irigoyen A, Torre P, DeAntoni G (2011) Physicochemical, microbial and sensory profiles of fermented milk containing probiotic strains isolated from kefir. J Dairy Res 78:456–463

    CAS  Google Scholar 

  86. Liu Z, Shen T, Zhang P, Ma Y, Qin H (2011) Lactobacillus plantarum surface layer adhesive protein protects internal epithelial cells against tight junction injury induced by enteropathogenic Escherichia coli. Mol Biol Rep 38:3471–3480

    CAS  Google Scholar 

  87. Lin WH, Wu CR, Fang TJ, Guo SYH, Lee MS, Yang HL (2011) Anti Helicobacter pylori activity of fermented milk with lactic acid bacteria. J Sci Food Agric 91:1424–1431

    CAS  Google Scholar 

  88. Oh Y, Osato M, Han X, Bennett G, Hong W (2002) Folk yoghurt kills Helicobacter pylori. J Appl Microbiol 93:1083–1088

    CAS  Google Scholar 

  89. Zubillaga M, Weill MR, Postaire E, Goldman C, Caro R, Boccio J (2001) Effect of probiotics and functional foods and their uses in different diseases. Nutr Res 21:569–579

    CAS  Google Scholar 

  90. Quirós A, Hernández-Ledesma M, Ramos M, Amigo L, Reclo I (2005) Angiotensin-converting enzyme inhibitory activity of peptides derived from caprine kefir. J Dairy Sci 88:3480–3487

    Google Scholar 

  91. Nakamura Y, Yamamoto N, Sakai K, Takano T (1995) Antihypertensive effect of sour milk and peptides isolated from it that are inhibitors to angiotensin I-converting enzyme. J Dairy Sci 78:1253–1257

    CAS  Google Scholar 

  92. Gilliland G, Nelson C, Maxwell C (1985) Assimilation of cholesterol by Lactobacillus acidophilus. Appl Enviro Microbiol 49(2):377–381

    CAS  Google Scholar 

  93. Vujičić I, Vulić M, Könyves T (1992) Assimilation of cholesterol in milk by kefir cultures. Biotechnol Lett 14(9):847–850

    Google Scholar 

  94. Klaver FA, van der Meer R (1993) The assumed assimilation of cholesterol by Lactobacilli and Bifidobacterium is due to their bile salt-deconjugating activity. Appl Environ Microbiol 59(4):1120–1124

    CAS  Google Scholar 

  95. Tahri K, Grill JP, Schneider F (1996) Bifidobacteria strain behavior towards cholesterol: coprecipitation with bile salts and assimilation. Curr Microbiol 33(3):187–193

    CAS  Google Scholar 

  96. Madani G, Mirlohi M, Yahay M, Hassanzadeh A (2013) How much in vitro cholesterol reducing activity of Lactobacilli predicts their in vivo cholesterol function? Int J Prev Med 4(4):404–413

    Google Scholar 

  97. St-Onge M, Farnworth E, Savard T, Chabot D, Mafu A, Jones P (2002) Kefir consumption does not alter plasma lipid levels or cholesterol fractional synthesis rates relative to milk in hyperlipidemic men: a randomized controlled trial. BMC Complement Altern Med 2:1

    Google Scholar 

  98. Liu JR, Wang SY, Chen MJ, Chen HL, Yueh PY, Lin CW (2006) Hypocholesterolaemic effects of milk-kefir and soyamilk-kefir in cholesterol-fed hamsters. Br J Nutr 95:939–946

    CAS  Google Scholar 

  99. Zheng Y, Lu Y, Wang J, Yang L, Pan C, Huang Y (2013) Probiotic properties of Lactobacillus strains isolated from Tibetan kefir. PLoS One 8(7):e698680

    Google Scholar 

  100. Maeda H, Zhu X, Suzuki S, Suzuki K, Kitamura S (2004) Structural characterization and biological activities of an exopolysaccharide kefiran produced by Lactobacillus kefiranofaciens WT-2B. J Agric Food Chem 52:5533–5538

    CAS  Google Scholar 

  101. Maeda H, Zhu Z, Omura K, Suzuki S, Kitamura S (2004) Effects of an exopolysaccharide (kefiran) on lipids, blood pressure, blood glucose, and constipation. BioFactors 22:197–200

    CAS  Google Scholar 

  102. Vinderola CG, Duarte J, Thangavel D, Perdigón G, Farnworth E, Matar C (2005) Immunomodulating capacity of kefir. J Dairy Res 72:195–202

    CAS  Google Scholar 

  103. Vinderola G, Perdigon G, Duarte J, Thangavel D, Farnworth E, Matar C (2006) Effects of kefir fractions on innate immunity. Immunobiology 21:149–156

    Google Scholar 

  104. Romanin D, Serradell M, Maciel DG, Lausada N, Garrote GL, Rumbo M (2010) Down-regulation of intestinal epithelial innate response by probiotic yeasts isolated from kefir. Int J Food Microbiol 140:102–108

    CAS  Google Scholar 

  105. Chen Y, Hsiao P, Hong W, Dai T, Chen M (2012) Lactobacillus kefiranofaciens M1 isolated from milk kefir grains ameliorates experimental colitis in vitro and in vivo. J Dairy Sci 95:63–74

    CAS  Google Scholar 

  106. Lee MY, Ahn KS, Kwon OK, Kim MJ, Kim MK, Lee IY, Oh SR, Lee HK (2007) Anti-inflammatory and anti-allergenic effects of kefir in a mouse asthma model. Immunobiology 212:647–654

    CAS  Google Scholar 

  107. de Moreno de LeBlanc A, Matar C, Farnworth E, Perdigón G (2006) Study of immune cells involved in the antitumor effect of kefir in a murine breast cancer model. J Dairy Sci 90:1920–1928

    Google Scholar 

  108. Maalouf K, Baydoun E, Rizk S (2011) Kefir induces cell-cycle arrest and apoptosis in HTLV-1-negative malignant T-lymphocytes. Cancer Manag Res 3:39–47. doi:10.2147/CMR.S15109

    CAS  Google Scholar 

  109. Gao J, Gu F, Ruan H, Chen Q, He J, He G (2012) Induction of apoptosis of gastric cancer cells SGC7901 In Vitro by a cell-free fraction of Tibetan kefir. Int Dairy J 30:14–18

    Google Scholar 

  110. Can C, Topuz E, Derin D, Durna Z, Aydinar A (2009) Effect of kefir on the quality of life of patients being treated for colorectal cancer. Oncol Nurs Forum 36(6):E335–E342

    Google Scholar 

  111. Topuz E, Derin D, Can G, Kürklü E, Çinar S, Aykan F, Çevikbaş A, Dişçi R, Durna Z, Şakar B, Saglam S, Tanyeri H, Deniz G, Gürer Ü, Taş F, Guney N, Aydinar A (2008) Effect of oral administration of kefir on serum proinflammatory cytokines on 5-FU induced oral mucositis in patients with colorectal cancer. Invest New Drugs 26:567–572

    CAS  Google Scholar 

  112. Alm L (1982) Effect of fermentation on lactose, glucose, and galactose content in milk and suitability of fermented milk products for lactose intolerant individuals. J Dairy Sci 65:346–352

    CAS  Google Scholar 

  113. Hertzler SR, Clancy SM (2003) Kefir improves lactose digestion and tolerance in adults with lactose maldigestion. J Am Diet Assoc 103:582–587

    Google Scholar 

  114. Alm L (1982) Effect of fermentation on L(+) and D(−) lactic acid in milk. J Dairy Sci 65:515–530

    CAS  Google Scholar 

  115. De Vrese M, Keller B, Barth CA (1992) Enhancement of intestinal hydrolysis of lactose by microbial β-galactosidase (EC 3.2.1.23) of kefir. Br J Nutr 67:67–75

    Google Scholar 

  116. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) (2012) Scientific opinion on the substantiation of health claims related to a combination of Lactobacillus acidophilus BCCM/LMG P-18806, Lactobacillus delbrueckii BCCM/LMG P-18805 and Streptococcus thermophilus BCCM/LMG P-18807 and reduction of gastro-intestinal. EFSA J 10(8):2855. Available online: www.efsa.europa.eu/efsajournal

  117. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA (2012) Scientific opinion on the substantiation of health claims related to non-characterized microorganisms (ID 2936, 2937,2938, 2941, 2944,2965, 2968, 2969, 3035, 3047, 3056, 3059, further assessment) pursuant to article 13(1) of regulation (EC) no. 1924/2006. EFSA J 10(8):2854

    Google Scholar 

  118. Chen K, Kuo C, Shiu J, Chen M (2011) Process optimization for a novel kefir candy with high probiotic viability. J Food Process Eng 34:427–443

    Google Scholar 

  119. Serafini F, Turroni F, Ruas-Madiedo P, Lugli GA, Milani C, Margolles A, Ventura M (2014) Kefir fermented milk and kefiran promote growth of Bifidobacterium bifidum PRL2010 and modulate its gene expression. Int J Food Microbiol 178:50–59

    CAS  Google Scholar 

  120. Patel S, Majumder A, Goyal A (2012) Potentials of exopolysaccharides from lactic acid bacteria. Indian J Microbiol 52(1):3–12

    CAS  Google Scholar 

  121. Rodrigues KL, Caputo LRG, Carvalho JCT, Evangelista J, Schneedorf JM (2005) Antimicrobial and healing activity of kefir andf kefiran extract. Int J Antimicrobial Agents 25:404–408

    CAS  Google Scholar 

  122. Rodrigues KL, Carvalho CT, Schneedorf JM (2005) Anti-inflammatory properties of kefir and its polysaccharide extract. Inflammopharmacology 13(5–6):485–492

    CAS  Google Scholar 

  123. Huseini H, Rahimzadeh G, Fazeli M, Mehrazma M, Salehi M (2012) Evaluation of wound healing activities of kefir products. Burns 38:719–723

    Google Scholar 

  124. Marshall VM, Rawson H (1999) Effects of exopolysaccharide-producing strains of thermophilic lactic acid bacteria on the texture of stirred yoghurt. Int J Food Sci Technol 34(2):137–143

    CAS  Google Scholar 

  125. Piermaria J, de la Cana IM, Abraham A (2008) Gelling properties of kefiran, a food grade polysaccharide obtained from kefir grains. Food Hydrocoll 22:1520–1527

    CAS  Google Scholar 

  126. Zajšek K, Goršek A, Kolar M (2013) Cultivating conditions effects on kefiran production by the mixed culture of lactic acid bacteria imbedded within kefir grains. Food Chem 139:970–977

    Google Scholar 

  127. Kourkoutas Y, Kandylis P, Panas P, Dooley J, Nigam P, Koutinas A (2006) Evaluation of freeze-dried kefir coculture as starter in feta-type cheese production. Appl Environ Microbiol 72(9):6124–6135

    CAS  Google Scholar 

  128. Goncu A, Alpkent Z (2005) Sensory and chemical properties of white pickled cheese produced using kefir, yoghurt, or a commercial cheese culture as a starter. Int Dairy J 15:771–776

    CAS  Google Scholar 

  129. Katechak IE, Panas P, Rapti K, Kandilogiannakis L, Koutinas A (2008) Production of hard-type cheese using free or immobilized freeze-dried kefir cells as a starter culture. J Agric Food Chem 56:5316–5323

    Google Scholar 

  130. Motedayen AA, Khodaiyan F, Saleh EA (2013) Development and characterization of composite films made of kefiran and starch. Food Chem 136:1231–1238

    CAS  Google Scholar 

  131. Piermaria JA, Pinotti A, Garcia MA, Abraham AG (2009) Films based on kefiran, an exopolysaccharide obtained from kefir grain: development and characterization. Food Hydrocoll 23:684–690

    CAS  Google Scholar 

  132. Piermaria J, Bosch A, Pinotti A, Yantorno O, Garcia MA, Abraham AG (2011) Kefiran films plasticized with sugars and polyols: water vapor barrier and mechanical properties in relation to their microstructure analyzed by ATR/FT-IR spectroscopy. Food Hydrocoll 25:1261–1269

    CAS  Google Scholar 

  133. Plessas S, Pherson L, Bekatorou A, Nigam P, Koutinas A (2005) Bread making using kefir grains as baker’s yeast. Food Chem 93:585–589

    CAS  Google Scholar 

  134. Plessas S, Trantallidi M, Bekatorou A, Kanellaki M, Nigam P, Koutinas AA (2007) Immobilization of kefir and Lactobacillus casei on brewery spent grains for use in sourdough wheat bread making. Food Chem 105:187–194

    CAS  Google Scholar 

  135. Mantzourani I, Plessas S, Saxami G, Alexopoulos A, Galanis A, Bezirtzoglou E (2014) Study of kefir grains application in sourdough bread regarding rope spoilage caused by Bacillus spp. Food Chem 143:17–21

    CAS  Google Scholar 

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Acknowledgments

Gülhan Ünlü thanks (1) J. William Fulbright Scholarship Board; (2) Institute of International Education—Council for International Exchange of Scholars (IIE-CIES); and (3) Fulbright Commission for Educational Exchange between the USA and Turkey for their generous support of her work as a Fulbright Scholar (2012–2013) at Middle East Technical University, Ankara, Turkey.

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The authors declare that they have no conflict of interest.

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Nielsen, B., Gürakan, G.C. & Ünlü, G. Kefir: A Multifaceted Fermented Dairy Product. Probiotics & Antimicro. Prot. 6, 123–135 (2014). https://doi.org/10.1007/s12602-014-9168-0

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