Abstract
TCRγδ+ T cells play a critical role in protecting the intestinal mucosa against pathogenic infection. In the absence of infection, TCRγδ+ T cell activation must be continuously regulated by T regulatory cells (Treg) to prevent the development of colitis. However, the activation of intestinal TCRγδ+ T cells under normal conditions has not been clearly resolved. In order to determine TCRγδ+ T cell activation in vivo, we designed an NF-κB based reporter system. Using the recombinant lentiviral method, we delivered the NF-κB reporter to isolated TCRγδ+ T cells, which were then adoptively transferred into normal mice. Our data indicate that the NF-κB activation level in TCRγδ+ T cells is higher in the intestinal intraepithelial layer than in the lamina propria region. In addition, the surface expression level of lymphocyte activation marker CD69 in TCRγδ+ T cells is also higher in the intestinal intraepithelial layer and this activation was reduced by Sulfatrim treatment which removes of commensal bacteria. Collectively, our data indicate that the TCRγδ+ T cell population attached to the intestinal lumen is constitutively activated even by normal commensal bacteria.
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Abbreviations
- TCR:
-
t cell receptor
- APC:
-
antigen presenting cell
- Treg:
-
regulatory T cell
- IEL:
-
intraepithelial lymphocyte
- LPL:
-
lamina propria lymphocyte
- LTR:
-
long terminal repeat
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Jeong, S.P., Kang, JA. & Park, SG. Intestinal intraepithelial TCRγδ+ T cells are activated by normal commensal bacteria. J Microbiol. 50, 837–841 (2012). https://doi.org/10.1007/s12275-012-2468-8
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DOI: https://doi.org/10.1007/s12275-012-2468-8