Abstract
The conserved band-interband pattern is thought to reflect the looped-domain organization of insect polytene chromosomes. Previously, we have shown that the chromodomain protein Chriz and the zinc-finger protein Z4 are essentially required for the maintenance of polytene chromosome structure. Here we show that both proteins form a complex that recruits the JIL-1 kinase to polytene chromosomes, enabling local H3S10 phosphorylation of interband nucleosomal histones. Interband targeting domains were identified at the N-terminal regions of Chriz and Z4, and our data suggest partial cooperation of the complex with the BEAF boundary element protein in polytene and diploid cells. Reducing the core component Chriz by RNAi results in destabilization of the complex and a strong reduction of interband-specific histone H3S10 phosphorylation.
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Acknowledgements
We thank Kristen M Johansen for her generous gift of JIL-1 antibodies, Ulrich K Laemmli for providing us with BEAF antibodies, and Andrey Gortchakov and Michael Cieslak for providing us with constructs. We are grateful to all our colleagues for providing their data to the Modencode data source in Flybase. MG was funded by a fellowship of the International PhD Program in Molecular Cell Biology that was provided by the MDC Berlin.
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[Gan M, Moebus S, Eggert H and Saumweber H 2011 The Chriz-Z4 complex recruits JIL-1 to polytene chromosomes, a requirement for interband-specific phosphorylation of H3S10. J. Biosci. 36 425–438] DOI 10.1007/s12038-011-9089-y
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Gan, M., Moebus, S., Eggert, H. et al. The Chriz–Z4 complex recruits JIL-1 to polytene chromosomes,a requirement for interband-specific phosphorylation of H3S10. J Biosci 36, 425–438 (2011). https://doi.org/10.1007/s12038-011-9089-y
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DOI: https://doi.org/10.1007/s12038-011-9089-y