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Quercetin Glycosides Induced Neuroprotection by Changes in the Gene Expression in a Cellular Model of Parkinson’s Disease

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Abstract

Quercetin glycosides, rutin and isoquercitrin, are potent antioxidants that have been found to possess neuroprotective effect in diseases like Parkinson’s and Alzheimer’s disease. In the present study, we have examined the gene expression changes with rutin and isoquercitrin pretreatment on 6-hydroxydopamine (6-OHDA)-treated toxicity in rat pheochromocytoma (PC12) cells. PC12 cells were pretreated with rutin or isoquercitrin and subsequently exposed to 6-OHDA. Rutin-pretreated PC12 attenuated the Park2, Park5, Park7, Casp3, and Casp7 genes which were expressed significantly in the 6-OHDA-treated PC12 cells. Rutin upregulated the TH gene which is important in dopamine biosynthesis, but isoquercitrin pretreatment did not affect the expression of this gene. Both rutin and isoquercitrin pretreatments upregulated the ion transport and antiapoptotic genes (NSF and Opa1). The qPCR array data were further validated by qRT-PCR using four primers, Park5, Park7, Casp3, and TH. This finding suggests that changes in the expression levels of transcripts encoded by genes that participate in ubiquitin pathway and dopamine biosynthesis may be involved in Parkinson’s disease.

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Authors and Affiliations

  1. Department of Pathology, Faculty of Medicine, International Medical University, Kuala Lumpur, Malaysia

    Kasthuri Bai Magalingam, Ammu Radhakrishnan & Premdass Ramdas

  2. Discipline of Physiology and Pharmacology, School of Veterinary and Biomedical Sciences, Faculty of Medicine, Health and Molecular Sciences, James Cook University, Townsville, 4811, Australia

    Nagaraja Haleagrahara

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  1. Kasthuri Bai Magalingam
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  2. Ammu Radhakrishnan
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  4. Nagaraja Haleagrahara
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Magalingam, K.B., Radhakrishnan, A., Ramdas, P. et al. Quercetin Glycosides Induced Neuroprotection by Changes in the Gene Expression in a Cellular Model of Parkinson’s Disease. J Mol Neurosci 55, 609–617 (2015). https://doi.org/10.1007/s12031-014-0400-x

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