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Effects of transplanted bone marrow mesenchymal stem cells on the irradiated intestine of mice

  • Original Paper
  • Published:
Journal of Biomedical Science

Abstract

We investigated the potency of exogenous bone marrow mesenchymal stem cells (MSCs) to engraft into irradiated intestine, as well as these cells’ effects on radiation-induced enteric injury. MSCs from β-Gal-transgenic mice were transplanted into C57BL/6J recipient mice that received abdominal irradiation (13 Gy). At different time points, recipient intestines were examined for the engraftment of donor-derived cells by immunofluorescence analysis. Additionally, the expression status of chemokines induced by radiation injury was analyzed in the irradiated intestine. Next, MSCs were transduced with an adenoviral vector encoding a certain chemokine receptor gene in order to promote the engraftment rate via chemotaxis. The intestinal permeability and histomorphological alterations were measured to evaluate the therapeutic effect of MSC transplantation. The results demonstrated that infused MSCs possessed the potency to engraft into irradiated enteric mucosa, but the engraftment rate was too low to produce a therapeutic effect. The expression of stromal cell-derived factor-1 (SDF-1) was up-regulated in irradiated intestine. MSCs genetically modified by CXCR4 (the receptor for SDF-1) engrafted into irradiated intestine at a significantly elevated level and ameliorated the intestinal permeability and histopathological damage.

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Acknowledgements

This study was supported by the Natural Science Foundation of Jiangsu Province (No. BK2007154).

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Authors and Affiliations

  1. Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, East Road 305, 210002, Nanjing, People’s Republic of China

    Jian Zhang, Jian-Feng Gong, Wei Zhang, Wei-Ming Zhu & Jie-Shou Li

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  1. Jian Zhang
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Correspondence to Wei-Ming Zhu.

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Zhang, J., Gong, JF., Zhang, W. et al. Effects of transplanted bone marrow mesenchymal stem cells on the irradiated intestine of mice. J Biomed Sci 15, 585–594 (2008). https://doi.org/10.1007/s11373-008-9256-9

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