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Genetic characterization of canine influenza A virus (H3N2) in Thailand

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Abstract

In January 2012, several clinical cases of dogs with flu-like symptoms, including coughing, sneezing, nasal discharge, and fever, were reported in a small-animal hospital located in Bangkok, Thailand. One influenza A virus was identified and characterized as an avian-like influenza virus H3N2. The virus was named A/canine/Thailand/CU-DC5299/12. A phylogenetic analysis indicated that the canine virus belonged to an avian Eurasian lineage and was genetically related to the canine influenza viruses H3N2 from China and Korea. This canine virus displays a unique genetic signature with two amino acid insertions in the NA protein, which is similar to the canine influenza viruses from eastern China (Zhejiang and Jiangsu). This study constitutes the first report of H3N2 canine influenza virus infection in a small-animal hospital in Thailand.

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Acknowledgments

This study has been funded in whole with federal funds from the National Institute of Allergy and Infectious Disease, National Institute of Health, Department of Health and Human Services, under Contract No. HHSN266200700007c. The study contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. The authors would like to thank Chulalongkorn University for its financial support in the Center of Excellence for Emerging and Re-emerging Infectious Diseases in Animals. The authors would like to thank also the National Research University of CHE and the Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University (HR1155A and RES560530129) for the grant of support to AA. The authors would like to thank Ms. Petra Hirsch for reviewing the manuscript.

Conflict of interest

The authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Center of Excellence for Emerging and Re-emerging Infectious Diseases in Animals, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Napawan Bunpapong, Nutthawan Nonthabenjawan, Supassama Chaiwong, Ratanaporn Tangwangvivat, Supanat Boonyapisitsopa, Waleemas Jairak, Ranida Tuanudom, Duangduean Prakairungnamthip, Sanipa Suradhat, Roongroje Thanawongnuwech & Alongkorn Amonsin

  2. Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Nutthawan Nonthabenjawan, Supassama Chaiwong, Ratanaporn Tangwangvivat, Waleemas Jairak & Alongkorn Amonsin

  3. Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Duangduean Prakairungnamthip & Sanipa Suradhat

  4. Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Roongroje Thanawongnuwech

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  1. Napawan Bunpapong
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Corresponding author

Correspondence to Alongkorn Amonsin.

Electronic supplementary material

Below is the link to the electronic supplementary material.

11262_2013_978_MOESM1_ESM.pptx

Phylogenetic analysis of the six internal genes of influenza A viruses. The Thai canine influenza H3N2 virus is presented as a circle. Supplementary material 1 (PPTX 256 kb)

11262_2013_978_MOESM2_ESM.pptx

The deduced amino acid sequences of the HA1 protein of a Thai canine influenza H3N2 viruses compared with canine, feline and avian H3N2 viruses. Supplementary material 2 (PPTX 620 kb)

11262_2013_978_MOESM3_ESM.pptx

The deduced amino acid sequences at the NA protein of a Thai canine influenza H3N2 viruses compared with canine, feline and avian H3N2 viruses. Supplementary material 3 (PPTX 731 kb)

11262_2013_978_MOESM4_ESM.docx

Nucleotide and amino acid identities (%) of the Thai canine H3N2 virus (CU-DC5299) compared with reference viruses. Supplementary material 4 (DOCX 100 kb)

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Bunpapong, N., Nonthabenjawan, N., Chaiwong, S. et al. Genetic characterization of canine influenza A virus (H3N2) in Thailand. Virus Genes 48, 56–63 (2014). https://doi.org/10.1007/s11262-013-0978-z

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