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Expression study of microRNA cluster on chromosome 19 (C19MC) in tumor tissue and serum of breast cancer patient

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Abstract

Background

Breast cancer (BC) is the most prevalent cancer among females worldwide. Numerous studies suggest that specific RNAs play a crucial role in carcinogenesis. The primate-specific microRNA gene cluster located on the 19q27.3 region of chromosome 19 (C19MC) could potentially regulate tumor cell proliferation, migration, and invasion.

Objective

The objective of this study was to compare the expression of miRNAs from the C19MC cluster in breast cancer tumor and non-tumor samples, as well as in the serum of individuals affected by BC and healthy individuals.

Methods

Peripheral blood was collected from 100 BC patients and 100 healthy individuals, and breast cancer samples including tumor and margin tissues were obtained. After RNA extraction, Real-time PCR was employed to investigate the expression of C19MC, specifically mir-515–1, mir-515–2, mir-516-A1, mir-516-A2, mir-516-B1, mir-516-B2, mir-517-A, mir-517-B, mir-517-C, and mir-518-A1, in the serum and tissue of BC patients and tumor margins. Statistical analyses and ROC curves were generated using GraphPad Prism software (v8.04), with a significance level set at p < 0.05.

Results

Our findings demonstrate a strong correlation between high expression of all C19MC miRNAs mentioned, except for mir-517-B, mir-517-C and mir- 518 in BC. These miRNAs show potential as notable non-invasive tumor markers.

Conclusion

The data obtained from our study support the overall impact of C19MC miRNAs in BC detection and emphasize the potential role of several C19MC members in this process.

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Data availability

All data analyzed during this study are included in this published article. Further raw data from the current study are available from the corresponding author upon request.

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Acknowledgements

We thank all patients and individuals for participating in this project.

Funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

    Saif Adil Ridha Altalebi, Mehdi Haghi & Mohammad Ali Hosseinpour Feizi

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  1. Saif Adil Ridha Altalebi
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  2. Mehdi Haghi
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  3. Mohammad Ali Hosseinpour Feizi
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Contributions

SARA Experimented and wrote the manuscript with support from MH and MAHF. MH supervised the work and contributed to the design and implementation of the research, the analysis of the results, and the writing of the manuscript. MAHF Helped supervise the project. All authors discussed the results and contributed and reviewed the final manuscript.

Corresponding author

Correspondence to Mehdi Haghi.

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Competing interest

The authors confirm that there are no known conflicts of interest associated with this publication, and there has been no significant financial support for this work that could have influencedits outcome.

Ethical approval

This study was approved by the Department of Biology committee at the University of Tabriz (14000907–198-7).

Consent to participate

All stages of sample collection have been done with the consent of patients and healthy people, and written consent forms have been obtained.

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Altalebi, S.A.R., Haghi, M. & Hosseinpour Feizi, M.A. Expression study of microRNA cluster on chromosome 19 (C19MC) in tumor tissue and serum of breast cancer patient. Mol Biol Rep 50, 9825–9831 (2023). https://doi.org/10.1007/s11033-023-08801-x

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