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The increased expression of DEC1 gene is related to HIF-1α protein in gastric cancer cell lines

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Abstract

Overexpression of differentiated embryo chondrocyte 1 (DEC1) has been reported to contribute to the cellular differentiation, proliferation, and apoptosis of various cancers. Our previous studies have shown that DEC1 was highly expressed in gastric cancer (GCa) tissues. However, there is no report about the expression of DEC1 in GCa cell lines until now. In this study, We evaluated the mRNA and protein expression of DEC1 and hypoxia-inducible factor 1α (HIF-1α) under normoxic and hypoxic conditions in six GCa cell lines: BGC-823, MGC80-3, MKN1, AGS, FU97 and SGC-7901. An HIF-1α protein inhibitor was used to analyze the association of DEC1 and HIF-1α expression. Under normoxia, the mRNA expression of both HIF-1α and DEC1 was moderate, whereas the protein expression of DEC1 was higher than that of HIF-1α. Hypoxia induced the mRNA expression of DEC1 and the protein expression of HIF-1α and DEC1 in a time-dependent manner but had no effect on the mRNA expression of HIF-1α. Furthermore, inhibition of HIF-1α protein expression resulted in a significant decrease in both the mRNA and protein expression of DEC1. Taken together, DEC1 expression is correlated with HIF-1α protein in GCa cell line, blockage of HIF-1α protein led to reduced DEC1 expression. The efficacy of inhibiting HIF-1α and DEC1 expression should be tested in clinical trials as possible treatment for GCa.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NSFC, No. 81000869), the “Spring City Scholars” construction project of Jinan City (Q2-06), the key projects of science and technology of Jinan City (No. 200807027).

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Authors and Affiliations

  1. Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, People’s Republic of China

    Yan Zheng, Min Wang & Yanfei Jia

  2. Shandong Province Key Lab of Tumor Target Molecule, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, People’s Republic of China

    Yan Zheng, Min Wang, Yanfei Jia & Yunshan Wang

  3. Department of Laboratory Medicine, Qianfoshan Hospital, Shandong University, Jinan, 250014, People’s Republic of China

    Xiaohong Shi

  4. Department of Laboratory, Weihai Municipal Hospital, Weihai, 264200, People’s Republic of China

    Binbin Li

  5. Qilu Hospital, Shandong University, Jinan, 250012, People’s Republic of China

    Yi Zhang

  6. Department of Respiratory, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People’s Republic of China

    Qinghua Liu

  7. Medical Research & Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, People’s Republic of China

    Yunshan Wang

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  1. Yan Zheng
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Correspondence to Qinghua Liu or Yunshan Wang.

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Yan Zheng and Xiaohong Shi contributed equally to this paper.

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Zheng, Y., Shi, X., Wang, M. et al. The increased expression of DEC1 gene is related to HIF-1α protein in gastric cancer cell lines. Mol Biol Rep 39, 4229–4236 (2012). https://doi.org/10.1007/s11033-011-1209-0

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