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Rapid Fluorescence Spectroscopic Characterization of Salivary DNA of Normal Subjects and OSCC Patients Using Ethidium Bromide

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Abstract

Recently, deoxyribonucleic acid (DNA) based biomarker(s) detection has been employed for cancer diagnosis. Earlier reports have suggested the presence of more DNA in the saliva of oral squamous cell carcinoma (OSCC) than normal by electrophoresis technique. Based on these, steady state and excited state kinetics of salivary DNA has been performed with 27 normal subjects and 67 OSCC patients saliva using ethidium bromide as a probe to look for the possibility in discrimination between them. On statistical analysis the sensitivity and specificity of 88.9 and 94.0 % has been achieved from the fluorescence emission spectra and 88.9 and 92.5 % with that of fluorescence excitation.

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Acknowledgments

The work was supported by DAE-BRNS Grant (Ref. No. 2009/34/38), Govt. of India. The author, M. Yuvaraj acknowledges DST-PURSE for fellowship. We acknowledge Dr. S. Sri Priya and Mrs. Arivazhagi of Government Arignar Anna Cancer Hospital, Regional Cancer Centre, Karapettai, Kanchipuram, for providing the samples and the histopathological reports.

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Authors and Affiliations

  1. Department of Medical Physics, Anna University, Chennai, 600 025, India

    Manoharan Yuvaraj, Prakasarao Aruna & Singaravelu Ganesan

  2. Department of Oral Medicine and Radiology, Meenakshi Ammal Dental College and Hospital, Chennai, 600 095, India

    Dornadula Koteeswaran

  3. Radiation Oncology, Dr. Rai Memorial Cancer Institute, Chennai, 600018, India

    Palraj Tamilkumar

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  1. Manoharan Yuvaraj
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  2. Prakasarao Aruna
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  3. Dornadula Koteeswaran
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  5. Singaravelu Ganesan
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Correspondence to Singaravelu Ganesan.

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Yuvaraj, M., Aruna, P., Koteeswaran, D. et al. Rapid Fluorescence Spectroscopic Characterization of Salivary DNA of Normal Subjects and OSCC Patients Using Ethidium Bromide. J Fluoresc 25, 79–85 (2015). https://doi.org/10.1007/s10895-014-1482-0

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  • DOI: https://doi.org/10.1007/s10895-014-1482-0

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