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Structure and Regulation of the V-ATPases

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The V-ATPases are ATP-dependent proton pumps present in both intracellular compartments and the plasma membrane. They function in such processes as membrane traffic, protein degradation, renal acidification, bone resorption and tumor metastasis. The V-ATPases are composed of a peripheral V1 domain responsible for ATP hydrolysis and an integral V0 domain that carries out proton transport. Our recent work has focused on structural analysis of the V-ATPase complex using both cysteine-mediated cross-linking and electron microscopy. For cross-linking studies, unique cysteine residues were introduced into structurally defined sites within the B and C subunits and used as points of attachment for the photoactivated cross-linking reagent MBP. Disulfide mediated cross-linking has also been used to define helical contact surfaces between subunits within the integral V0 domain. With respect to regulation of V-ATPase activity, we have investigated the role that intracellular environment, luminal pH and a unique domain of the catalytic A subunit play in controlling reversible dissociation in vivo.

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Authors and Affiliations

  1. Department of Physiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, Massachusetts, 02111

    Takao Inoue, Yanru Wang, Kevin Jefferies, Jie Qi, Ayana Hinton & Michael Forgac

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  1. Takao Inoue
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  2. Yanru Wang
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Correspondence to Michael Forgac.

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Inoue, T., Wang, Y., Jefferies, K. et al. Structure and Regulation of the V-ATPases. J Bioenerg Biomembr 37, 393–398 (2005). https://doi.org/10.1007/s10863-005-9478-8

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