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Sickness Behavior Score Is Associated with Neuroinflammation and Late Behavioral Changes in Polymicrobial Sepsis Animal Model

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Abstract

The use of reliable scores is a constant development in critical illness. According to Sepsis-3 consensus, the use of Sequential Organ Failure Assessment (SOFA) score of 2 or more is associated with a higher mortality of sepsis patients. In experimental research, due murine animal model limitations, the use of a score systems can be an alternative to assess sepsis severity. In this work, we suggest a sickness behavior score (SBS) that uses physiological variables to assess sepsis severity and mortality. Animals were evaluated daily by the presence of six indicators of sickness behavior: temperature alteration, preference of water/sucrose, liquid intake, food intake, body weight, and movimentation. Male adult Wistar rats were evaluated daily after sepsis induction by cecal ligation and puncture (CLP) or laparotomy only (sham) for determination of SBS. Oxidative stress, IL-6, and HPA axis markers (corticosterone and adrenal gland weight) were evaluated 24 h after CLP to determine the correlation with the acute SBS and neuroinflammation. Also, BDNF and four cognitive behavioral tests were correlated with the chronic SBS, i.e., sum of 8 days after surgery. In result, septic rats presented higher SBS than sham animals. Sepsis severity markers were associated with acute and chronic SBS. Also, SBS was negative correlated with the cognitive tests. In conclusion, SBS shows to be reliable score to predict sepsis severity and mortality. The use of score system provides the analysis of global sickness behavior, beyond evaluation of each parameter individually.

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Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.

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Authors and Affiliations

  1. Laboratory of Neurobiology of Inflammatory and Metabolic Processes (NEUROIMet), Graduate Program in Health Sciences, University of South Santa Catarina, Tubarão, SC, Brazil

    Mariana Pereira de Souza Goldim, Amanda Della Giustina, Khiany Mathias, Aloir de Oliveira Junior, Maria Eduarda Fileti, Raquel De Carli, Graciela Zarbato, Leandro Garbossa, Naiana da Rosa, Juliana Oliveira, Jucelia Fortunato & Fabricia Petronilho

  2. Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, University of Southern Santa Catarina, Criciúma, SC, Brazil

    Andriele Vieira, Jaqueline Generoso, Tatiana Barichello & Felipe Dal-Pizzol

  3. Experimental Neuroscience Laboratory (LaNEx), Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Palhoça, Santa Catarina, Brazil

    Bruna Hoffmann Oliveira, Nivaldo Ferreira, Juliete Palandi, Franciane Bobinski & Daniel Fernandes Martins

  4. Center of Excellence on Mood Disorders, Department of Psychiatry and Behavioral Sciences, McGovern Medical School, The University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA

    Tatiana Barichello & Joao Quevedo

  5. Translational Psychiatry Program, Department of Psychiatry and Behavioral Sciences, McGovern Medical School, The University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA

    Tatiana Barichello & Joao Quevedo

  6. Neuroscience Graduate Program, Graduate School of Biomedical Sciences, The University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA

    Joao Quevedo

  7. Laboratory of Translational Psychiatry, Graduate Program in Health Sciences, University of Southern Santa Catarina, Criciúma, SC, 88806-000, Brazil

    Joao Quevedo

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  1. Mariana Pereira de Souza Goldim
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Pereira de Souza Goldim, M., Della Giustina, A., Mathias, K. et al. Sickness Behavior Score Is Associated with Neuroinflammation and Late Behavioral Changes in Polymicrobial Sepsis Animal Model. Inflammation 43, 1019–1034 (2020). https://doi.org/10.1007/s10753-020-01187-z

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