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Expression and redistribution of β-catenin in the cardiac myocytes of left ventricle of spontaneously hypertensive rat

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Abstract

Beta-catenin is not only an adhering junction protein, but also the central player of the canonical Wnt signalling pathway. In order to investigate the roles of β-catenin in the mechanism of myocardial hypertrophy, we determined the expression and distribution of β-catenin in the cardiomyocytes of spontaneously hypertensive heart failure (SHHF) rats and age-matched Wistar-Kyoto (WKY) rats. We identified the reducing of β-catenin expression in the membrane protein fraction but increasing in the nuclear protein in the 6 and 12 month-old SHHF rats as compared with the age-matched WKY rats by Western blotting. Immunolabeling of β-catenin colocalized with cadherin at the intercalated disc sites and showed nuclear accumulation in myocytes of SHHF rats. We also revealed that the association between glycogen synthase kinase-3β and β-catenin had weakened in the 6 month-old SHHF rats as compared with the age-matched WKY rats by immunoprecipitation. These findings suggested that nuclear translocation of β-catenin might play important roles in regulating signal transduction in the decompensated hypertrophy stage.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (30871046) and the Guangdong Natural Science Foundation (8151008901000162).

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Authors and Affiliations

  1. Department of Pathology, Sun Yat-sen University the Fifth Affiliated Hospital, 52 Meihua E. Road, Zhuhai, 519000, Guangdong, People’s Republic of China

    Qiaoli Zheng, Zeqing Xu & Xian Ping Yi

  2. Department of Pathology, Jiangmen Central Hospital, 23 Haipang N.Street, Jiangmen, 529030, Guangdong, People’s Republic of China

    Ping Chen

  3. Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, 601 Elmwood Avenue, Box 626, Room G5264F, Rochester, NY, 14642, USA

    Faqian Li

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  1. Qiaoli Zheng
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  2. Ping Chen
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Correspondence to Xian Ping Yi.

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Zheng, Q., Chen, P., Xu, Z. et al. Expression and redistribution of β-catenin in the cardiac myocytes of left ventricle of spontaneously hypertensive rat. J Mol Hist 44, 565–573 (2013). https://doi.org/10.1007/s10735-013-9507-6

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  • DOI: https://doi.org/10.1007/s10735-013-9507-6

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