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A ThCAP gene from Tamarix hispida confers cold tolerance in transgenic Populus (P. davidiana × P. bolleana)

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Abstract

The ThCAP gene, which encodes a cold acclimation protein, was isolated from a Tamarix hispida NaCl-stress root cDNA library; its expression patterns were then assayed by qRT-PCR in different T. hispida tissues treated with low temperature (4°C), salt (400 mM NaCl), drought (20% PEG6000) and exogenous abscisic acid (100 μM). Induction of ThCAP gene was not only responsive to different stress conditions but was also organ specific. When transgenic Populus (P. davidiana × P. bolleana) plants were generated, expressing ThCAP under regulation of the cauliflower mosaic virus CaMV 35S promoter, they had a greater resistance to low temperature than non-transgenic seedlings, suggesting that ThCAP might play an important role in cold tolerance.

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Acknowledgements

This study was supported by National Natural Science Foundation (Grant No. 30571509), Heilongjiang province scientific and technological project (Grant No. GB06B303 and WB07N02).

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Authors and Affiliations

  1. Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 150040, Harbin, China

    Xiao-Hong Guo, Jing Jiang, Shi-Jie Lin, Bai-Chen Wang, Yu-Cheng Wang, Gui-Feng Liu & Chuan-Ping Yang

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  1. Xiao-Hong Guo
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  2. Jing Jiang
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  3. Shi-Jie Lin
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  4. Bai-Chen Wang
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  5. Yu-Cheng Wang
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  7. Chuan-Ping Yang
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Correspondence to Jing Jiang.

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Guo, XH., Jiang, J., Lin, SJ. et al. A ThCAP gene from Tamarix hispida confers cold tolerance in transgenic Populus (P. davidiana × P. bolleana). Biotechnol Lett 31, 1079–1087 (2009). https://doi.org/10.1007/s10529-009-9959-7

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  • DOI: https://doi.org/10.1007/s10529-009-9959-7

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