Abstract
Notch signaling is closely related to cell proliferation, cell apoptosis, cell fate decisions, DNA damage repair, and so on. However, the exactly regulatory mechanism of Notch signaling pathway in liver regeneration (LR) remains unclear. To reveal the role of Notch signaling pathway in rat liver regeneration, Ingenuity Pathway Analysis (IPA) software and related pathway database were firstly used to construct the Notch signaling pathway in this study. Next, eight type cells with high purity were obtained by Percoll density centrifugation and immunomagnetic beads sorting. Then, the expression profiles of Notch signaling pathway-related genes in eight type cells were checked by using Rat Genome 230 2.0 Array, and the results showed that the expression of 42 genes were significantly regulated. H-cluster results showed that the hepatic stellate cells are attributed to one cluster; hepatocyte cell, oval cell, sinusoidal endothelial cell, and Kupffer cell are clustered together; and biliary epithelial cell, pit cell, and dendritic cell are one cluster. IPA software and Expression analysis systematic explorer analysis indicated that Notch signaling pathway-related genes were involved in cell proliferation, apoptosis, cell cycle, DNA damage repair, etc. In conclusion, Notch signaling pathway might regulate various physiological activities of LR through multiple pathways.
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Acknowledgments
This study was funded by National Natural Science Foundation Project of China [No. U1404312], Science and technology project of Henan Province [Nos. 172102310507, 182300410334], Foundation for young backbone teachers in Colleges and universities in Henan [Nos. 2017GGJS045], Doctoral Scientific Research Start-Up Foundation of HNU [No. qd13033], and Youth Teachers Foundation of HNU [No. 2014].
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X Yang designed the study; C Xu provided the data; C He, L Zhu, W Zhao, S Li, C Xia analyzed the data; X Yang, C He contributed to the writing of the manuscript.
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10528_2018_9869_MOESM1_ESM.xlsx
Rat Genome230 2.0 microarray detection dates of Notch signaling pathway-related genes in eight liver cell types during LR. The values ≥ 2 are marked with red ground and represent significantly up-regulated expression of genes; those ≤ 0.5 are marked with green ground and represent significantly down-regulated expression of genes. Supplementary material 1 (XLSX 73Kb)
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Yang, X., He, C., Zhu, L. et al. Comparative Analysis of Regulatory Role of Notch Signaling Pathway in 8 Types Liver Cell During Liver Regeneration. Biochem Genet 57, 1–19 (2019). https://doi.org/10.1007/s10528-018-9869-2
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DOI: https://doi.org/10.1007/s10528-018-9869-2