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B7-H4 expression in various tumors determined using a novel developed monoclonal antibody

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Abstract

B7-H4, a new member of the B7 family, may participate in the negative regulation of cell-mediated immunity, while aberrant B7-H4 expression is detected in some tumors and it participates in the occurrence and development of the tumors. In this study, we developed one monoclonal antibody (mAb) whose clone No. was 4H8 against the extracellular domains of B7-H4 through immunization of Balb/c mice with the 3T3-mB7-H4 cells that expressed extrinsic B7-H4 stably. And we detected the expression characteristics of B7-H4 in various tumors using 4H8 mAb by enzyme-linked immunosorbent assay (ELISA), immunoprecipitation and immunohistochemistry (IHC) analysis. B7-H4 expression was significantly higher in the tumor tissues derived from uterus, breast, and colon than in their corresponding normal tissues. Further, the B7-H4 expression was related to the stage of the tumors. In contrast, B7-H4 expression did not differ significantly between the tumor tissues derived from the stomach and liver and the normal tissues. Different expression levels of B7-H4 in the tumors indicated that B7-H4 may be involved in tumor formation and development. Specific mAbs against B7-H4 will be useful in studying the role of B7-H4 in tumor pathogenesis and pathological process.

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Acknowledgments

This work was supported by a grant (No. 30972777) from the National Natural Science Foundation of China (to HPY). This paper is proofread by a native English professional with science background at Elixigen Corporation.

Conflict of interest

The authors declare that they have no conflict of interest related to the publication of this manuscript.

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Authors and Affiliations

  1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, 310003, Hangzhou, China

    Yun Qian, Ling Shen, Linfang Cheng, Zhigang Wu & Hangping Yao

  2. Institute of Immunology, Zhejiang University, 310058, Hangzhou, China

    Yun Qian

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  1. Yun Qian
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  2. Ling Shen
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  3. Linfang Cheng
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  4. Zhigang Wu
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  5. Hangping Yao
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Correspondence to Hangping Yao.

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Qian, Y., Shen, L., Cheng, L. et al. B7-H4 expression in various tumors determined using a novel developed monoclonal antibody. Clin Exp Med 11, 163–170 (2011). https://doi.org/10.1007/s10238-010-0125-2

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  • DOI: https://doi.org/10.1007/s10238-010-0125-2

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