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An improvement in acute wound healing in mice by the combined application of photobiomodulation and curcumin-loaded iron particles

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Abstract

Here, we examined the combined effect of pulse wave photobiomodulation (PBM) with curcumin-loaded superparamagnetic iron oxide (Fe3O4) nanoparticles (curcumin), in an experimental mouse model of acute skin wound. Thirty male adult mice were randomly allocated into 5 groups. Group 1 was served as the control group. Group 2 was a placebo and received distilled water, as a carrier of curcumin. Group 3 received laser (890 nm, 80 Hz, 0.2 J/cm2). Group 4 received curcumin by taking four injections around the wound. Group 5 received laser + curcumin. One full-thickness excisional round wound was made on the back of all the mice. On days 0, 4, 7, and 14, bacterial flora, wound surface area, and tensile strength were examined and microbiological examinations were performed. In case of wound closure, the two-way ANOVA shows that wound surface area of entire groups decreased progressively. However, the decrease in laser + curcumin and laser groups, and especially data from laser + curcumin group were statistically more significant, in comparison with the other groups (F statistics = 2.28, sig = 0.019). In terms of microbiology, the two-way ANOVA showed that laser, and laser + curcumin groups have statistically a lower bacterial count than the curcumin, control, and carrier groups (F statistics = 35, sig = 0 = 000). Finally, the one-way ANOVA showed that laser + curcumin, curcumin, and curcumin significantly increased wound strength, compared to the control and carrier groups. Furthermore, laser + curcumin significantly increased wound strength, compared to the control, laser, and curcumin groups (LSD test, p = 0.003, p = 0.002, and p = 0.005, respectively). In conclusion, curcumin nanoparticles, pulse wave laser, and pulse wave laser + curcumin nanoparticles accelerate wound healing, through a significant increase in wound closure rate, as well as wound strength, and a significant decrease in Staphylococcus aureus counts. Furthermore, the statistical analysis of our data suggests that the combined treatment of pulse wave laser + curcumin nanoparticles enhances the wound closure rate, and wound strength, compared to the laser and curcumin nanoparticles alone.

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Acknowledgments

The authors declare no financial support for this paper. Supported in part by NIH grant DK105692.

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Authors and Affiliations

  1. Department of Biology and Anatomical sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Ali Moradi, Mohammad-Amin Abdollahifar & Abdollah Amini

  2. School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Yashar Kheirollahkhani, Payam Fatahi & Mohammad Bagheri

  3. Department of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Parvaneh Naserzadeh

  4. Radiation Biology Research Center, Iran University of Medical Sciences, Tehran, Iran

    Khadijah Ashtari

  5. Department of Medical Nanotechnology, Faculty of Advanced Technology in Medicine, Iran University of Medical Sciences, Tehran, Iran

    Khadijah Ashtari

  6. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

    Khadijah Ashtari

  7. Department of Statistics, University of Qom, Qom, Iran

    Seyed Kamran Ghoreishi

  8. Price Institute of Surgical Research, University of Louisville, and Noveratech LLC of Louisville, Louisville, KY, 40202, USA

    Sufan Chien

  9. Best Journal Center, P.O. Box 16315-365, Tehran, Iran

    Fatemehalsadat Rezaei

  10. Department of Biology and Anatomical Sciences, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

    Mohammadjavad Fridoni

  11. Department of Microbiology, School of medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Sudabeh Taheri

  12. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 1985717443, Iran

    Mohammad Bayat

  13. Price Institute of Surgical Research, University of Louisville, and Noveratech LLC of Louisville, Louisville, KY, 40202, USA

    Mohammad Bayat

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  1. Ali Moradi
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Correspondence to Sufan Chien or Mohammad Bayat.

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All methods were accepted by the Medical Ethics Committee of Shahid Behehti University of Medical Sciences (IRB approval number: IR.SBMU.MSP/REC.1395.9124).

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Moradi, A., Kheirollahkhani, Y., Fatahi, P. et al. An improvement in acute wound healing in mice by the combined application of photobiomodulation and curcumin-loaded iron particles. Lasers Med Sci 34, 779–791 (2019). https://doi.org/10.1007/s10103-018-2664-9

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