Skip to main content

Advertisement

Springer Nature Link
Log in

Correlation between immunohistochemical staining of CEACAM1 and clinicopathological findings in oral pre-neoplastic lesions and squamous cell carcinoma

  • Original Paper
  • Published:
Medical Molecular Morphology Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Squamous cell carcinoma of the oral cavity represents the sixth most common cancer worldwide and it is often preceded by pre-neoplastic lesions. Sometimes it is still difficult for pathologists to make objective differential diagnoses only on histological characteristics. Tumorigenesis is accompanied by altered expression of cell adhesion molecules, like carcinoembryonic antigen cell adhesion molecule (CEACAM)1. We wanted to investigative CEACAM1 in oral dysplastic lesions, carcinoma in situ (CIS) and oral squamous cell carcinoma (OSCC). We examined immunohistochemical CEACAM1 expression in 50 OSCC, 30 oral CIS and 40 pre-neoplastic lesions and assessed its correlation with clinical and pathological parameters. CEACAM1 was not expressed in normal mucosa, significantly expressed in CIS while it was negative in all the dysplastic lesions. In OSCC, high CEACAM1 expression was associated with tumor grade and inversely correlated with both overall and disease-specific 5-year survival. We showed that CEACAM1 expression is very dynamic: absent in dysplastic lesions, up-regulated in CIS and OSCC. We suggest that CEACAM1 could be a prognostic marker of OSCC and oral CIS. Our most important finding was that it could help pathologists diagnosing oral carcinoma in situ.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Canada)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D (2011) Global cancer statistics. CA Cancer J Clin 61:69–90

    Article  PubMed  Google Scholar 

  2. Shah JP, Lydiatt W (1995) Treatment of cancer of the head and neck. CA Cancer J Clin 45:352–368

    Article  CAS  PubMed  Google Scholar 

  3. Hunter KD, Parkinson EK, Harrison PR (2005) Profiling early head and neck cancer. Nat Rev Cancer 5:127–135

    Article  CAS  PubMed  Google Scholar 

  4. Cavallaro U, Christofori G (2001) Cell adhesion in tumor invasion and metastasis: loss of the glue is not enough. Biochim Biophys Acta 1552:39–45

    CAS  PubMed  Google Scholar 

  5. Thompson JA, Grunert F, Zimmermann W (1991) Carcinoembryonic gene family: molecular biology and clinical perspectives. J Clin Lab Anal 5:344–366

    Article  CAS  PubMed  Google Scholar 

  6. Scheffrahn I, Singer BB, Sigmundsson K, Lucka L, Obrink B (2005) Control of density-dependent, cell state-specific signal transduction by the cell adhesion molecule CEACAM1, and its influence on cell cycle regulation. Exp Cell Res 307:427–435

    Article  CAS  PubMed  Google Scholar 

  7. Sienel W, Dango S, Woelfle U, Morresi-Hauf A, Wagener C, Brümmer J, Mutschler W, Passlick B, Pantel K (2003) Elevated expression of carcinoembryonic antigen-related cell adhesion molecule 1 promotes progression of non-small cell lung cancer. Clin Cancer Res 9:2260–2266

    CAS  PubMed  Google Scholar 

  8. Oliveira-Ferrer L, Tilki D, Ziegeler G, Hauschild J, Loges S, Irmak S, Kilic E, Huland H, Friedrich M, Ergün S (2004) Dual role of carcinoembryonic antigen-related cell adhesion molecule 1 in angiogenesis and invasion of human urinary bladder cancer. Cancer Res 64:8932–8938

    Article  CAS  PubMed  Google Scholar 

  9. Wang F, Guan B, Yang J, Wang H, Zhou C (2017) CEACAM1 is overexpressed in oral tumors and related to tumorigenesis. Med Mol Morphol 50:42–51

    Article  CAS  PubMed  Google Scholar 

  10. Barnes H, Eveson John W, Reichart P, Sidransky D (2005) WHO: pathology and genetics, head and neck tumours: tumours of the oral cavity and oropharynx. IARC Press, Lyon

    Google Scholar 

  11. Kuespert K, Pils S, Hauck CR (2006) CEACAMs: their role in physiology and pathophysiology. Curr Opin Cell Biol 18:565–571

    Article  CAS  PubMed  Google Scholar 

  12. Beauchemin N, Arabzadeh A (2013) Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) in cancer progression and metastasis. Cancer Metastasis Rev 32:643–671

    Article  CAS  PubMed  Google Scholar 

  13. Fiori V, Magnani M, Cianfriglia M (2012) The expression and modulation of CEACAM1 and tumor cell transformation. Ann Ist Super Sanità 48:161–171

    Article  CAS  PubMed  Google Scholar 

  14. Shinozuka K, Uzawa K, Fushimi K, Yamano Y, Shiiba M, Bukawa H, Yokoe H, Tanzawa H (2009) Downregulation of carcinoembryonic antigen-related cell adhesion molecule 1 in oral squamous cell carcinoma: correlation with tumor progression and poor prognosis. Oncology 76:387–397

    Article  CAS  PubMed  Google Scholar 

  15. Zhou CJ, Qu X, Yang YM, Wang FF, Dong ZQ, Wang CQ, Zhang XY, Liu GX, Wei FC, Sun SZ (2009) CEACAM1 distribution and its effects on angiogenesis and lymphangiogenesis in oral carcinoma. Oral Oncol 45:883–886

    Article  CAS  PubMed  Google Scholar 

  16. Wang N, Feng Y, Wang Q, Liu S, Xiang L, Sun M, Zhang X, Liu G, Qu X, Wei F (2014) Neutrophils infiltration in the tongue squamous cell carcinoma and its correlation with CEACAM1 expression on tumor cells. PLoS One 27:e89991

    Article  Google Scholar 

  17. Bamberger AM, Kappes H, Methner C, Rieck G, Brümmer J, Wagener C, Löning T, Milde-Langosch K (2002) Expression of the adhesion molecule CEACAM1 (CD66a, BGP, C-CAM) in breast cancer is associated with the expression of the tumor-suppressor genes Rb, Rb2, and p27. Virchows Arch 440:139–144

    Article  CAS  PubMed  Google Scholar 

  18. Nittka S, Günther J, Ebisch C, Erbersdobler A, Neumaier M (2004) The human tumor suppressor CEACAM1 modulates apoptosis and is implicated in early colorectal tumorigenesis. Oncogene 23:9306–9313

    Article  CAS  PubMed  Google Scholar 

  19. Albarran-Somoza B, Franco-Topete R, Delgado-Rizo V, Cerda-Camacho F, Acosta-Jimenez L, Lopez-Botet M, Daneri-Navarro A (2006) CEACAM1 in cervical cancer and precursor lesions: association with human papillomavirus infection. J Histochem Cytochem 54:1393–1399

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Busch C, Hanssen TA, Wagener C, Obrink B (2002) Down-regulation of CEACAM1 in human prostate cancer: correlation with loss of cell polarity, increased proliferation rate, and Gleason grade 3 to 4 transition. Human Pathol 33:290–298

    Article  CAS  Google Scholar 

  21. Cruz PV, Wakai T, Shirai Y, Yokoyama N, Hatakeyama K (2005) Loss of carcinoembryonic antigen-related cell adhesion molecule 1 expression is an adverse prognostic factor in hepatocellular carcinoma. Cancer 104:354–360

    Article  CAS  PubMed  Google Scholar 

  22. Gale N, Zidar N, Poljak M, Cardesa A (2014) Current views and perspectives on classification of squamous intraepithelial lesions of the head and neck. Head Neck Pathol 8:16–23

    Article  PubMed  PubMed Central  Google Scholar 

  23. Zhang HK, Liu HG (2012) Is severe dysplasia the same lesion as carcinoma in situ? 10-Year follow-up of laryngeal precancerous lesions. Acta Otolaryngol 132:325–328

    Article  PubMed  Google Scholar 

  24. Toshiyuki I (2011) Oral premalignant lesions: from the pathological viewpoint. Int J Clin Oncol 6:15–26

    Google Scholar 

  25. Kobayashi T, Maruyama S, Cheng J, Ida-Yonemochi H, Yagi M, Takagi R, Saku T (2010) Histopathological varieties of oral carcinoma in situ: diagnosis aided by immunohistochemistry dealing with the second basal cell layer as the proliferating center of oral mucosal epithelia. Pathol Int 60:156–166

    Article  PubMed  Google Scholar 

  26. Mikami T, Cheng J, Maruyama S, Kobayashi T, Funayama A, Yamazaki M, Adeola HA, Wu L, Shingaki S, Saito C, Saku T (2011) Emergence of keratin 17 vs. loss of keratin 13: their reciprocal immunohistochemical profiles in oral carcinoma in situ. Oral Oncol 47:497–503

    Article  CAS  PubMed  Google Scholar 

  27. Funayama A, Cheng J, Maruyama S, Yamazaki M, Kobayashi T, Syafriadi M, Kundu S, Shingaki S, Saito C, Saku T (2011) Enhanced expressions of podoplanin in oral carcinomas in situ and squamous cell carcinomas. Pathobiology 78:171–180

    Article  CAS  PubMed  Google Scholar 

  28. Nozawa H, Chiu C, Hanahan D (2006) Infiltrating neutrophils mediate the initial angiogenic switch in a mouse model of multistage carcinogenesis. Proc Natl Acad Sci USA 103:12493–12498

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Coussens LM, Werb Z (2002) Inflammation and cancer. Nature 420:860–867

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Chen Z, Chen L, Baker K, Olszak T, Zeissig S, Huang YH, Kuo TT, Mandelboim O, Beauchemin N, Lanier LL (2011) CEACAM1 dampens antitumor immunity by down-regulating NKG2D ligand expression on tumor cells. J Exp Med 208:2633–2640

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Gebauer F, Wicklein D, Horst J, Sundermann P, Maar H, Streichert T, Tachezy M, Izbicki JR, Bockhorn M, Schumacher U (2014) Carcinoembryonic antigen-related cell adhesion molecules (CEACAM) 1, 5 and 6 as biomarkers in pancreatic cancer. PLoS One 19:e113023

    Article  Google Scholar 

Download references

Author information

Author notes

  1. Oriana Simonetti and Guendalina Lucarini equally contributed to this work.

Authors and Affiliations

  1. Department of Dermatology, Polytechnic University of Marche, Torrette, Ancona, Italy

    Oriana Simonetti & Anna Maria Offidani

  2. Department of Clinic and Molecular Sciences, Histology, Polytechnic University of Marche, Via Tronto 10/a, Torrette, 60020, Ancona, Italy

    Guendalina Lucarini & Roberto Di Primio

  3. Department of Biomedical Sciences and Public Health, Section of Pathologic Anatomy and Histopathology, Polytechnic University of Marche, Torrette, Ancona, Italy

    Corrado Rubini & Antonio Zizzi

  4. Dental Clinic Private Practice, Pesaro, Italy

    Simone Domenico Aspriello

Authors
  1. Oriana Simonetti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guendalina Lucarini
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Corrado Rubini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Antonio Zizzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Simone Domenico Aspriello
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Roberto Di Primio
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Anna Maria Offidani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guendalina Lucarini.

Ethics declarations

Conflict of interest

The authors declared no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Simonetti, O., Lucarini, G., Rubini, C. et al. Correlation between immunohistochemical staining of CEACAM1 and clinicopathological findings in oral pre-neoplastic lesions and squamous cell carcinoma. Med Mol Morphol 51, 41–47 (2018). https://doi.org/10.1007/s00795-017-0169-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00795-017-0169-4

Keywords

Search

Navigation