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Identification of a novel member of the genus Laulavirus (family Phenuiviridae) from the entomopathogenic ascomycete fungus Cordyceps javanica

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Abstract

The virus family Phenuiviridae (order Hareavirales, comprising segmented negative-sense single stranded RNA viruses) has highly diverse members that are known to infect animals, plants, protozoans, and fungi. In this study, we identified a novel phenuivirus infecting a strain of the entomopathogenic fungus Cordyceps javanica isolated from a small brown plant hopper (Laodelphax striatellus), and this virus was tentatively named "Cordyceps javanica negative-strand RNA virus 1" (CjNRSV1). The CjNRSV1 genome consists of three negative-sense single stranded RNA segments (RNA1–3) with lengths of 7252, 2401, and 1117 nt, respectively. The 3′- and 5′-terminal regions of the RNA1, 2, and 3 segments have identical sequences, and the termini of the RNA segments are complementary to each other, reflecting a common characteristic of viruses in the order Hareavirales. RNA1 encodes a large protein (∼274 kDa) containing a conserved domain for the bunyavirus RNA-dependent RNA polymerase (RdRP) superfamily, with 57–80% identity to the RdRP encoded by phenuiviruses in the genus Laulavirus. RNA2 encodes a protein (∼79 kDa) showing sequence similarity (47–63% identity) to the movement protein (MP, a plant viral cell-to-cell movement protein)-like protein (MP-L) encoded by RNA2 of laulaviruses. RNA3 encodes a protein (∼28 kDa) with a conserved domain of the phenuivirid nucleocapsid protein superfamily. Phylogenetic analysis using the RdRPs of various phenuiviruses and other unclassified phenuiviruses showed CjNRSV1 to be grouped with established members of the genus Laulavirus. Our results suggest that CjNRSV1 is a novel fungus-infecting member of the genus Laulavirus in the family Phenuiviridae.

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Data availability

The complete nucleotide sequences of CjNRSV1 been deposited in the NCBI GenBank database under the accession numbers PP544309, PP544310, and PP544311.

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Acknowledgements

The authors thank Zhang Yan and Qi Yuhua for assistance with collection of insect samples.

Funding

This research was supported by National Natural Science Foundation of China (31970159, 32001867) and the Natural Science Foundation of Shandong Province of China (ZR2020QC129).

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Authors and Affiliations

  1. College of Plant Health and Medicine, Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, Qingdao Agricultural University, Qingdao, 266109, China

    Xinran Cao, Bo Liu, Ziqi Wang, Ida Bagus Andika & Shengqi Chi

  2. College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, 271018, China

    Xinran Cao

  3. Shouguang International Vegetable Sci-Tech Fair Management Service Center, Shouguang, 262700, China

    Xinran Cao

  4. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Xianyang, 712100, China

    Tianxing Pang & Liying Sun

  5. Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046, Japan

    Hideki Kondo

  6. Key Laboratory of Biotechnology in Plant Protection of MARA, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China

    Junmin Li & Ida Bagus Andika

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  1. Xinran Cao
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Correspondence to Ida Bagus Andika or Shengqi Chi.

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Cao, X., Liu, B., Wang, Z. et al. Identification of a novel member of the genus Laulavirus (family Phenuiviridae) from the entomopathogenic ascomycete fungus Cordyceps javanica. Arch Virol 169, 166 (2024). https://doi.org/10.1007/s00705-024-06069-5

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