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Recombinant HCMV UL128 expression and functional identification of PBMC-attracting activity in vitro

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An Erratum to this article was published on 12 December 2012

Abstract

Human cytomegalovirus (HCMV) has evolved several immune evasion strategies. One strategy is controlling the movement of peripheral blood mononuclear cells (PBMCs) by encoding homologues of chemokines. Our aim was to determine whether HCMV open reading frame (ORF) UL128 could encode a protein that attracts PBMCs like a β-chemokine. The recombinant UL128 protein was synthesized by construction of a stably transfected CHO-UL128 cell line, and a chemotaxis assay showed that UL128 was able to attract PBMCs with a potency equal to that of MIP-1α in vitro. We hypothesize that UL128 protein may act as a β-chemokine homologue in viral pathogenesis.

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References

  1. Akter P, Cunningham C, McSharry BP, Dolan A, Addison C, Dargan DJ, Hassan-Walker AF, Emery VC, Griffiths PD, Wilkinson GW, Davison AJ (2003) Two novel spliced genes in human cytomegalovirus. J Gen Virol 84:1117–1122

    Article  PubMed  CAS  Google Scholar 

  2. Baldanti F, Paolucci S, Campanini G, Sarasini A, Percivalle E, Revello MG, Gerna G (2006) Human cytomegalovirus UL131A, UL130 and UL128 genes are highly conserved among field isolates. Arch Virol 151:1225–1233

    Article  PubMed  CAS  Google Scholar 

  3. Hahn G, Revello MG, Patrone M, Percivalle E, Campanini G, Sarasini A, Wagner M, Gallina A, Milanesi G, Koszinowski U, Baldanti F, Gerna G (2004) Human cytomegalovirus UL131-128 genes are indispensable for virus growth in endothelial cells and virus transfer to leukocytes. J Virol 78:10023–10033

    Article  PubMed  CAS  Google Scholar 

  4. Mantovani A (1999) The chemokine system: redundancy for robust outputs. Immunol Today 20:254–257

    Article  PubMed  CAS  Google Scholar 

  5. Murphy PM (2001) Viral exploitation and subversion of the immune system through chemokine mimicry. Nat Immunol 2:116–122

    Article  PubMed  CAS  Google Scholar 

  6. Patrone M, Secchi M, Bonaparte E, Milanesi G, Gallina A (2007) Cytomegalovirus UL131-128 products promote gB conformational transition and gB-gH interaction during entry into endothelial cells. J Virol 81:11479–11488

    Article  PubMed  CAS  Google Scholar 

  7. Penfold ME, Dairaghi DJ, Duke GM, Saederup N, Mocarski ES, Kemble GW, Schall TJ (1999) Cytomegalovirus encodes a potent alpha chemokine. Proc Natl Acad Sci USA 96:9839–9844

    Article  PubMed  CAS  Google Scholar 

  8. Reckless J, Grainger DJ (1999) Identification of oligopeptide sequences which inhibit migration induced by a wide range of chemokines. Biochem J 340:803–811

    Article  PubMed  CAS  Google Scholar 

  9. Revello MG, Gerna G (2010) Human cytomegalovirus tropism for endothelial/epithelial cells: scientific background and clinical implications. Rev Med Virol 20:136–155

    Article  PubMed  CAS  Google Scholar 

  10. Rossi D, Zlotnik A (2000) The biology of chemokines and their receptors. Annu Rev Immunol 18:217–242

    Article  PubMed  CAS  Google Scholar 

  11. Ryckman BJ, Chase MC, Johnson DC (2008) HCMV gH/gL/UL128-131 interferes with virus entry into epithelial cells: evidence for cell type-specific receptors. Proc Natl Acad Sci USA 105:14118–14123

    Article  PubMed  CAS  Google Scholar 

  12. Ryckman BJ, Jarvis MA, Drummond DD, Nelson JA, Johnson DC (2006) Human cytomegalovirus entry into epithelial and endothelial cells depends on genes UL128 to UL150 and occurs by endocytosis and low-pH fusion. J Virol 80:710–722

    Article  PubMed  CAS  Google Scholar 

  13. Ryckman BJ, Rainish BL, Chase MC, Borton JA, Nelson JA, Jarvis MA, Johnson DC (2008) Characterization of the human cytomegalovirus gH/gL/UL128-131 complex that mediates entry into epithelial and endothelial cells. J Virol 82:60–70

    Article  PubMed  CAS  Google Scholar 

  14. Straschewski S, Patrone M, Walther P, Gallina A, Mertens T, Frascaroli G (2011) Protein pUL128 of human cytomegalovirus is necessary for monocyte infection and blocking of migration. J Virol 85:5150–5158

    Article  PubMed  CAS  Google Scholar 

  15. Tiffany HL, Lautens LL, Gao JL, Pease J, Locati M, Combadiere C, Modi W, Bonner TI, Murphy PM (1997) Identification of CCR8: a human monocyte and thymus receptor for the CC chemokine I-309. J Exp Med 186:165–170

    Article  PubMed  CAS  Google Scholar 

  16. Vischer HF, Leurs R, Smit MJ (2006) HCMV-encoded G-protein-coupled receptors as constitutively active modulators of cellular signaling networks. Trends Pharmacol Sci 27:56–63

    Article  PubMed  CAS  Google Scholar 

  17. Wang D, Shenk T (2005) Human cytomegalovirus virion protein complex required for epithelial and endothelial cell tropism. Proc Natl Acad Sci USA 102:18153–18158

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by research grants from the National Natural Science Foundation of China (No. 81071337), the Natural Science Foundation of Zhejiang Province (No. Z2110006) and the General Project of Education Department of Zhejiang Province (No. Y201017974). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Zhejiang Key Laboratory for Diagnosis and Therapy of neonatal Diseases, Key Laboratory of Reproductive Genetics (Zhejiang University) Ministry of Education, Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China

    Gao Hui-hui, Tao Ran, Zheng Qi, Xu Jun & Shang Shi-qiang

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  1. Gao Hui-hui
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  2. Tao Ran
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  3. Zheng Qi
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Correspondence to Shang Shi-qiang.

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Hui-hui, G., Ran, T., Qi, Z. et al. Recombinant HCMV UL128 expression and functional identification of PBMC-attracting activity in vitro . Arch Virol 158, 173–177 (2013). https://doi.org/10.1007/s00705-012-1378-8

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