Abstract
Avian reovirus (ARV) is an important cause of disease in poultry. Although ARV is known to induce apoptosis in infected cells, the interaction between ARV and its target cells requires further elucidation. In this report, we show that the ARV isolate strain GX/2010/1 induces autophagy in both Vero and primary chicken embryonic fibroblast (CEF) cells based on the appearance of an increased number of double-membrane vesicles, the presence of GFP-microtubule-associated protein 1 light chain 3 (GFP-LC3) dot formation, and the elevated production of LC3II. We further demonstrate that the class I phosphoinositide 3-kinase (PI3K)/Akt/mTOR pathway contributes to autophagic induction by ARV infection. Moreover, treatment of ARV-infected cells with the autophagy inducer rapamycin increased viral yields, while inhibition of the autophagosomal pathway using chloroquine led to a decrease in virus production. Altogether, our studies strongly suggest that autophagy may play a critical role in determining viral yield during ARV infection.
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Acknowledgments
This work was supported by grants from the State Key Laboratory of Veterinary Biotechnology of China (SKLVBF201109), the China Agriculture Research System (CARS-41-K08), and the Program for Changjiang Scholars and Innovative Research Teams in University (IRT0978).
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S. Meng and K. Jiang contributed equally to this work.
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Meng, S., Jiang, K., Zhang, X. et al. Avian reovirus triggers autophagy in primary chicken fibroblast cells and Vero cells to promote virus production. Arch Virol 157, 661–668 (2012). https://doi.org/10.1007/s00705-012-1226-x
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DOI: https://doi.org/10.1007/s00705-012-1226-x