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Prevalence and genetic characterization of avian polyomavirus and psittacine beak and feather disease virus isolated from budgerigars in Mainland China

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Abstract

Budgerigar fledgling disease (BFD) and psittacine beak and feather disease (PBFD) are caused by avian polyomavirus (APV) and psittacine beak and feather disease virus (PBFDV), respectively. These diseases frequently infect psittacine birds and result in similar clinical manifestations. In this study, we observed the prevalence of PBFDV infection and a dual infection of APV and PBFDV in a budgerigar (Melopsittacus undulatus) in Mainland China for the first time. One PBFDV isolate and two APV isolates were harvested using chicken embryos. Genetic characterization and phylogenetic analysis of the complete genome of the two APV isolates revealed nucleotide similarity ranging from 99.0% to 99.6% to other sequences in GenBank, and a 14-bp insertion was observed in the genome of one APV isolate. The results of complete genome analysis of the PBFDV isolate showed nucleotide similarity ranging from 83.0% to 95.0% with other PBFDV sequences in GenBank. Genetic characterization and phylogenetic analysis of the APV and PBFDV strains isolated in this study indicated that the isolates from China were closely related to their Japanese counterparts. The results of this study will help to identify molecular determinants and will aid further research on the prevention and control of APV and PBFD infection.

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Authors and Affiliations

  1. Laboratory of Animal Epidemiological Surveillance, China Animal Health and Epidemiology Center, Qingdao, 266032, China

    Qingye Zhuang, Jiming Chen, Jie Chen, Shuo Liu, Guangyu Hou, Jinping Li, Baoxu Huang & Wenming Jiang

  2. Department of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, Pakistan

    Muhammad Hassan Mushtaq

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  1. Qingye Zhuang
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  2. Jiming Chen
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  3. Muhammad Hassan Mushtaq
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  4. Jie Chen
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  5. Shuo Liu
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Correspondence to Wenming Jiang.

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Zhuang, Q., Chen, J., Mushtaq, M.H. et al. Prevalence and genetic characterization of avian polyomavirus and psittacine beak and feather disease virus isolated from budgerigars in Mainland China. Arch Virol 157, 53–61 (2012). https://doi.org/10.1007/s00705-011-1138-1

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  • DOI: https://doi.org/10.1007/s00705-011-1138-1

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