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Pichindé virus is trafficked through a dynamin 2 endocytic pathway that is dependent on cellular Rab5- and Rab7-mediated endosomes

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Abstract

Pichindé virus (PICV) is a New World arenavirus that has been shown to enter cells through a clathrin-dependent endocytic pathway. In this study, we determined that PICV is trafficked through the cellular dynamin 2 (dyn2) endocytic pathway. Additionally, the data suggest that PICV entry is pH-dependent and that the virus travels through Rab5-mediated early and Rab7-mediated late endosomes. In all, this study characterizes the endocytic pathway utilized by the arenavirus PICV.

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References

  1. Borrow P, Oldstone M (1994) Mechanism of lymphocytic choriomeningitis virus entry into cells. Virology 198:1–9

    Article  PubMed  CAS  Google Scholar 

  2. Cao W, Henry M, Borrow P, Yamada H, Elder J, Ravkov E, Nichol S, Compans R, Campbell K, Oldstone M (1998) Identification of alpha-dystoglygan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus. Science 282:2079–2081

    Article  PubMed  CAS  Google Scholar 

  3. Colpitts T, Moore A, Kolokoltsov A, Davey R (2007) Venezuelan equine encephalitis virus infection of mosquito cells requires acidification as well as mosquito homologs of the endocytic proteins Rab5 and Rab7. Virology 369:78–91

    Article  Google Scholar 

  4. Duan D, Li Q, Kao A, Yue Y, Pessin J, Engelhardt J (1999) Dynamin is required for recombinant adeno-associated virus type 2 infection. J Virol 73:10371–10376

    PubMed  CAS  Google Scholar 

  5. Glushakova S, Lukashevich I (1989) Early events in arenavirus replication are sensitive to lysosomotropic compounds. Arch Virol 104:157–161

    Article  PubMed  CAS  Google Scholar 

  6. Guiducci C, Ott G, Chan J, Damon E, Calacsan C, Matray T, Lee K, Coffman R, Barrat F (2006) Properties regulating the nature of the plasmacytoid dendritic cell response to Toll-like receptor 9 activation. J Exp Med 203:1999–2008

    Article  PubMed  CAS  Google Scholar 

  7. Jordens I, Marsman M, Kuijl C, Neefljes J (2005) Rab proteins, connecting transport and vesicle fusion. Traffic 6:1070–1077

    Article  PubMed  CAS  Google Scholar 

  8. Kolokoltsov A, Fleming E, Davey R (2006) Venezuelan equine encephalitis virus entry mechanism requires late endosome formation and resists cell membrane cholesterol depletion. Virology 347:333–342

    Article  PubMed  CAS  Google Scholar 

  9. Krishnan M, Sukumaran B, Pal U, Agaisse H, Murray J, Hodge T, Fikrig E (2007) Rab5 is required for the cellular entry of Dengue and West Nile viruses. J Virol 81:4881–4885

    Article  PubMed  CAS  Google Scholar 

  10. Marsh M, Helenius A (2006) Virus entry: open sesame. Cell 124:729–740

    Article  PubMed  CAS  Google Scholar 

  11. Martinez M, Cordo S, Candurra N (2007) Characterization of Junin arenavirus cell entry. J Gen Virol 88:1776–1784

    Article  PubMed  CAS  Google Scholar 

  12. Maxfield F, McGraw T (2004) Endocytic recycling. Nat Rev Mol Cell Biol 5:121–132

    Article  PubMed  CAS  Google Scholar 

  13. McCormick J (1999) Lassa fever. In: Saluzzo J, Dodet B (eds) Emergence and control of rodent-borne viral diseases (hantaviral and arenal diseases). Elsevier, Paris, pp 177–195

  14. McNiven M, Cao H, Pitts K, Yoon Y (2000) The dynamin family of mechanoenzymes: pinching in new places. Trends Biochem Sci 25:115–120

    Article  PubMed  CAS  Google Scholar 

  15. Pietiainen V, Marjomaki V, Upla P, Pelkmans L, Helenius A, Hyypia T (2004) Echovirus 1 endocytosis into caveosomes requires lipid rafts, dynamin II, and signaling events. Mol Biol Cell 15:4911–4925

    Article  PubMed  Google Scholar 

  16. Radoshitzky S, Abraham J, Spiropoulou C, Kuhn J, Nguyen D, Wenhui L, Nagel J, Schmidt P, Nunberg J, Andrews N, Farzan M, Choe H (2007) Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses. Nature 446:92–96

    Article  PubMed  CAS  Google Scholar 

  17. Sauvonnet N, Dujeancourt A, Dautry-Varsat A (2005) Cortactin and dynamin are required for the clathrin-independent endocytosis of γc cytokine receptor. J Cell Biol 168:155–163

    Article  PubMed  CAS  Google Scholar 

  18. Stein M, Dong J, Wandinger-Ness A (2003) Rab proteins and endocytic trafficking: potential targets for therapeutic intervention. Adv Drug Deliv Rev 55:1421–1437

    Article  PubMed  CAS  Google Scholar 

  19. Tesh R, Salas R, Fulhorst C, de Manzione N, Duno G, Weaver S, Utera A, Paredes H, Ellis B, Mills J, Bowen M, Ksiazek T (1999) Epidemiology of arenaviruses in the Americas. In: Saluzzo J, Dodet B (eds) Factors in the emergence and control of rodent-borne viral diseases (hantaviral and arenal diseases). Elsevier, Paris, pp 213–221

  20. Vela E, Bowick G, Herzog N, Aronson J (2008) Genistein treatment of cells inhibits arenavirus infection. Antiviral Res 77:153–156

    Article  PubMed  CAS  Google Scholar 

  21. Vela E, Zhang L, Colpitts T, Davey R, Aronson J (2007) Arenavirus entry occurs through a cholesterol-dependent, non-caveolar, clathrin-mediated endocytic mechanism. Virology 369:1–11

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We would like to thank Eugene Knutson for his assistance with confocal microscopy. This work was supported by the NIAID Training in Emerging and Reemerging Infectious Diseases T32 AI007536. Robert A. Davey is supported by grants from NIAID 1R01AI063513-01A2 and U54 AI057156.

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Authors and Affiliations

  1. Department of Pathology, Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, TX, USA

    Eric M. Vela, Lihong Zhang & Judith F. Aronson

  2. Department of Microbiology and Immunology, Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, TX, USA

    Tonya M. Colpitts & Robert A. Davey

  3. Battelle Biomedical Research Center (BBRC), Battelle Memorial Institute, 505 King Avenue, JM8-1-096, Columbus, OH, 43201-2693, USA

    Eric M. Vela

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  1. Eric M. Vela
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  2. Tonya M. Colpitts
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  3. Lihong Zhang
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  4. Robert A. Davey
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  5. Judith F. Aronson
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Correspondence to Eric M. Vela.

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Vela, E.M., Colpitts, T.M., Zhang, L. et al. Pichindé virus is trafficked through a dynamin 2 endocytic pathway that is dependent on cellular Rab5- and Rab7-mediated endosomes. Arch Virol 153, 1391–1396 (2008). https://doi.org/10.1007/s00705-008-0129-3

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