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Expression of sapovirus virus-like particles in mammalian cells

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Summary.

Sapovirus (SaV) is an etiological agent of acute gastroenteritis in human and swine. SaV can be divided into five genogroups, GI to GV. Virus-like particles (VLPs) morphologically similar to native SaV have been expressed for GI, GII, GIII and GV strains in insect cells, although only low expression levels were observed for GII strains. In this study, we report the successful expression of SaV GII VLPs using cultured mammalian COS-7 and 293T cells. Our results demonstrated that this mammalian expression system was able to express and form SaV VLPs.

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References

  • KO Chang SV Sosnovtsev G Belliot Y Kim LJ Saif KY Green (2004) ArticleTitleBile acids are essential for porcine enteric calicivirus replication in association with down-regulation of signal transducer and activator of transcription 1. Proc Natl Acad Sci USA 101 8733–8738 Occurrence Handle1:CAS:528:DC%2BD2cXltFKksbg%3D Occurrence Handle15161971

    CAS  PubMed  Google Scholar 

  • R Chen JD Neill JS Noel AM Hutson RI Glass MK Estes BV Prasad (2004) ArticleTitleInter- and intragenus structural variations in caliciviruses and their functional implications. J Virol 78 6469–6479 Occurrence Handle1:CAS:528:DC%2BD2cXkvVCjsLc%3D Occurrence Handle15163740

    CAS  PubMed  Google Scholar 

  • IN Clarke PR Lambden (2000) ArticleTitleOrganization and expression of calicivirus genes. J Infect Dis 181 IssueID[Suppl] 2 309–316

    Google Scholar 

  • T Farkas WM Zhong Y Jing PW Huang SM Espinosa N Martinez AL Morrow GM Ruiz-Palacios LK Pickering X Jiang (2004) ArticleTitleGenetic diversity among sapoviruses. Arch Virol 149 1309–1323 Occurrence Handle10.1007/s00705-004-0296-9 Occurrence Handle1:CAS:528:DC%2BD2cXltVKms70%3D Occurrence Handle15221533

    Article  CAS  PubMed  Google Scholar 

  • WT Flynn LJ Saif (1988) ArticleTitleSerial propagation of porcine enteric calicivirus-like virus in primary porcine kidney cell cultures. J Clin Microbiol 26 206–212 Occurrence Handle1:STN:280:BieC3sbntFw%3D Occurrence Handle2830305

    CAS  PubMed  Google Scholar 

  • TR Fuerst EG Niles FW Studier B Moss (1986) ArticleTitleEukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci USA 83 8122–8126 Occurrence Handle1:CAS:528:DyaL2sXhtlak Occurrence Handle3095828

    CAS  PubMed  Google Scholar 

  • KY Green T Ando MS Balayan T Berke IN Clarke MK Estes DO Matson S Nakata JD Neill MJ Studdert HJ Thiel (2000) ArticleTitleTaxonomy of the caliciviruses. J Infect Dis 181 IssueIDSuppl 2 S322–S330 Occurrence Handle10804145

    PubMed  Google Scholar 

  • M Guo Y Qian KO Chang LJ Saif (2001) ArticleTitleExpression and self-assembly in baculovirus of porcine enteric calicivirus capsids into virus-like particles and their use in an enzyme-linked immunosorbent assay for antibody detection in swine. J Clin Microbiol 39 1487–1493 Occurrence Handle10.1128/JCM.39.4.1487-1493.2001 Occurrence Handle1:STN:280:DC%2BD3Mvnt1yjug%3D%3D Occurrence Handle11283075

    Article  CAS  PubMed  Google Scholar 

  • GS Hansman K Katayama N Maneekarn S Peerakome P Khamrin S Tonusin S Okitsu O Nishio N Takeda H Ushijima (2004) ArticleTitleGenetic diversity of norovirus and sapovirus in hospitalized infants with sporadic cases of acute gastroenteritis in Chiang Mai, Thailand. J Clin Microbiol 42 1305–1307 Occurrence Handle10.1128/JCM.42.3.1305-1307.2004 Occurrence Handle15004104

    Article  PubMed  Google Scholar 

  • GS Hansman K Katayama T Oka K Natori N Takeda (2005) ArticleTitleMutational study of sapovirus expression in insect cells. Virol J 2 13 Occurrence Handle10.1186/1743-422X-2-13 Occurrence Handle15727685

    Article  PubMed  Google Scholar 

  • GS Hansman K Natori T Oka S Ogawa K Tanaka N Nagata H Ushijima N Takeda K Katayama (2005) ArticleTitleCross-reactivity among sapovirus recombinant capsid proteins. Arch Virol 150 21–36 Occurrence Handle1:CAS:528:DC%2BD2MXksVKisg%3D%3D Occurrence Handle15449145

    CAS  PubMed  Google Scholar 

  • GS Hansman K Natori H Ushijima K Katayama N Takeda (2005) ArticleTitleCharacterization of polyclonal antibodies raised against sapovirus genogroup five virus-like particles. Arch Virol 150 1433–1437 Occurrence Handle1:CAS:528:DC%2BD2MXltl2msbs%3D Occurrence Handle15770355

    CAS  PubMed  Google Scholar 

  • X Jiang W Zhong M Kaplan LK Pickering DO Matson (1999) ArticleTitleExpression and characterization of Sapporo-like human calicivirus capsid proteins in baculovirus. J Virol Methods 78 81–91 Occurrence Handle10.1016/S0166-0934(98)00169-4 Occurrence Handle1:CAS:528:DyaK1MXhsVWmtLk%3D Occurrence Handle10204699

    Article  CAS  PubMed  Google Scholar 

  • K Katayama T Miyoshi K Uchino T Oka T Tanaka N Takeda GS Hansman (2004) ArticleTitleNovel recombinant sapovirus. Emerg Infect Dis 10 1874–1876 Occurrence Handle1:CAS:528:DC%2BD2cXpsFSqtLw%3D Occurrence Handle15504283

    CAS  PubMed  Google Scholar 

  • MA Mayo (2002) ArticleTitleA summary of taxonomic changes recently approved by ICTV. Arch Virol 147 1655–1663 Occurrence Handle1:CAS:528:DC%2BD38XovVamu7s%3D Occurrence Handle12181683

    CAS  PubMed  Google Scholar 

  • K Numata ME Hardy S Nakata S Chiba MK Estes (1997) ArticleTitleMolecular characterization of morphologically typical human calicivirus Sapporo. Arch Virol 142 1537–1552 Occurrence Handle10.1007/s007050050178 Occurrence Handle1:CAS:528:DyaK2sXlslOrtbc%3D Occurrence Handle9672617

    Article  CAS  PubMed  Google Scholar 

  • T Oka K Katayama S Ogawa GS Hansman T Kageyama H Ushijima T Miyamura N Takeda (2005) ArticleTitleProteolytic processing of sapovirus ORF1 polyprotein. J Virol 79 7283–7290 Occurrence Handle10.1128/JVI.79.12.7283-7290.2005 Occurrence Handle1:CAS:528:DC%2BD2MXkvFCnurw%3D Occurrence Handle15919882

    Article  CAS  PubMed  Google Scholar 

  • S Robinson IN Clarke IB Vipond EO Caul PR Lambden (2002) ArticleTitleEpidemiology of human Sapporo-like caliciviruses in the South West of England: molecular characterisation of a genetically distinct isolate. J Med Virol 67 282–288 Occurrence Handle10.1002/jmv.2219 Occurrence Handle1:CAS:528:DC%2BD38XktlGmsrc%3D Occurrence Handle11992591

    Article  CAS  PubMed  Google Scholar 

  • I Schuffenecker T Ando D Thouvenot B Lina M Aymard (2001) ArticleTitleGenetic classification of “Sapporo-like viruses”. Arch Virol 146 2115–2132 Occurrence Handle10.1007/s007050170024 Occurrence Handle1:CAS:528:DC%2BD38XovFWh Occurrence Handle11765915

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan

    T. Oka, G. S. Hansman, K. Katayama, S. Ogawa, T. Miyamura & N. Takeda

  2. Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan

    N. Nagata

Authors
  1. T. Oka
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  2. G. S. Hansman
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  3. K. Katayama
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  4. S. Ogawa
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  5. N. Nagata
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  6. T. Miyamura
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  7. N. Takeda
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Oka, T., Hansman, G., Katayama, K. et al. Expression of sapovirus virus-like particles in mammalian cells. Arch Virol 151, 399–404 (2006). https://doi.org/10.1007/s00705-005-0613-y

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  • DOI: https://doi.org/10.1007/s00705-005-0613-y

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